Chitin Nerve Conduits with Three-Dimensional Spheroids of Mesenchymal Stem Cells from SD Rats Promote Peripheral Nerve Regeneration

Peripheral nerve injury (PNI) is an unresolved medical problem with limited therapeutic effects. Epineurium neurorrhaphy is an important method for treating PNI in clinical application, but it is accompanied by inevitable complications such as the misconnection of nerve fibers and neuroma formation....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ci Li, Meng Zhang, Song-Yang Liu, Feng-Shi Zhang, Teng Wan, Zhen-Tao Ding, Pei-Xun Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	peripheral nerve injury chitin peripheral nerve conduits bone marrow mesenchymal stem cells spheroid Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/51501b47e3bc4fc4b3eb8882fc21bffa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:51501b47e3bc4fc4b3eb8882fc21bffa
record_format	dspace
spelling	oai:doaj.org-article:51501b47e3bc4fc4b3eb8882fc21bffa2021-11-25T18:48:45ZChitin Nerve Conduits with Three-Dimensional Spheroids of Mesenchymal Stem Cells from SD Rats Promote Peripheral Nerve Regeneration10.3390/polym132239572073-4360https://doaj.org/article/51501b47e3bc4fc4b3eb8882fc21bffa2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/3957https://doaj.org/toc/2073-4360Peripheral nerve injury (PNI) is an unresolved medical problem with limited therapeutic effects. Epineurium neurorrhaphy is an important method for treating PNI in clinical application, but it is accompanied by inevitable complications such as the misconnection of nerve fibers and neuroma formation. Conduits small gap tubulization has been proved to be an effective suture method to replace the epineurium neurorrhaphy. In this study, we demonstrated a method for constructing peripheral nerve conduits based on the principle of chitosan acetylation. In addition, the micromorphology, mechanical properties and biocompatibility of the chitin nerve conduits formed by chitosan acetylation were further tested. The results showed chitin was a high-quality biological material for constructing nerve conduits. Previous reports have demonstrated that mesenchymal stem cells culture as spheroids can improve the therapeutic potential. In the present study, we used a hanging drop protocol to prepare bone marrow mesenchymal stem cell (BMSCs) spheroids. Meanwhile, spherical stem cells could express higher stemness-related genes. In the PNI rat model with small gap tubulization, BMSCs spheres exhibited a higher ability to improve sciatic nerve regeneration than BMSCs suspension. Chitin nerve conduits with BMSCs spheroids provide a promising therapy option for peripheral nerve regeneration.Ci LiMeng ZhangSong-Yang LiuFeng-Shi ZhangTeng WanZhen-Tao DingPei-Xun ZhangMDPI AGarticleperipheral nerve injurychitinperipheral nerve conduitsbone marrow mesenchymal stem cellsspheroidOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3957, p 3957 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	peripheral nerve injury chitin peripheral nerve conduits bone marrow mesenchymal stem cells spheroid Organic chemistry QD241-441
spellingShingle	peripheral nerve injury chitin peripheral nerve conduits bone marrow mesenchymal stem cells spheroid Organic chemistry QD241-441 Ci Li Meng Zhang Song-Yang Liu Feng-Shi Zhang Teng Wan Zhen-Tao Ding Pei-Xun Zhang Chitin Nerve Conduits with Three-Dimensional Spheroids of Mesenchymal Stem Cells from SD Rats Promote Peripheral Nerve Regeneration
description	Peripheral nerve injury (PNI) is an unresolved medical problem with limited therapeutic effects. Epineurium neurorrhaphy is an important method for treating PNI in clinical application, but it is accompanied by inevitable complications such as the misconnection of nerve fibers and neuroma formation. Conduits small gap tubulization has been proved to be an effective suture method to replace the epineurium neurorrhaphy. In this study, we demonstrated a method for constructing peripheral nerve conduits based on the principle of chitosan acetylation. In addition, the micromorphology, mechanical properties and biocompatibility of the chitin nerve conduits formed by chitosan acetylation were further tested. The results showed chitin was a high-quality biological material for constructing nerve conduits. Previous reports have demonstrated that mesenchymal stem cells culture as spheroids can improve the therapeutic potential. In the present study, we used a hanging drop protocol to prepare bone marrow mesenchymal stem cell (BMSCs) spheroids. Meanwhile, spherical stem cells could express higher stemness-related genes. In the PNI rat model with small gap tubulization, BMSCs spheres exhibited a higher ability to improve sciatic nerve regeneration than BMSCs suspension. Chitin nerve conduits with BMSCs spheroids provide a promising therapy option for peripheral nerve regeneration.
format	article
author	Ci Li Meng Zhang Song-Yang Liu Feng-Shi Zhang Teng Wan Zhen-Tao Ding Pei-Xun Zhang
author_facet	Ci Li Meng Zhang Song-Yang Liu Feng-Shi Zhang Teng Wan Zhen-Tao Ding Pei-Xun Zhang
author_sort	Ci Li
title	Chitin Nerve Conduits with Three-Dimensional Spheroids of Mesenchymal Stem Cells from SD Rats Promote Peripheral Nerve Regeneration
title_short	Chitin Nerve Conduits with Three-Dimensional Spheroids of Mesenchymal Stem Cells from SD Rats Promote Peripheral Nerve Regeneration
title_full	Chitin Nerve Conduits with Three-Dimensional Spheroids of Mesenchymal Stem Cells from SD Rats Promote Peripheral Nerve Regeneration
title_fullStr	Chitin Nerve Conduits with Three-Dimensional Spheroids of Mesenchymal Stem Cells from SD Rats Promote Peripheral Nerve Regeneration
title_full_unstemmed	Chitin Nerve Conduits with Three-Dimensional Spheroids of Mesenchymal Stem Cells from SD Rats Promote Peripheral Nerve Regeneration
title_sort	chitin nerve conduits with three-dimensional spheroids of mesenchymal stem cells from sd rats promote peripheral nerve regeneration
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/51501b47e3bc4fc4b3eb8882fc21bffa
work_keys_str_mv	AT cili chitinnerveconduitswiththreedimensionalspheroidsofmesenchymalstemcellsfromsdratspromoteperipheralnerveregeneration AT mengzhang chitinnerveconduitswiththreedimensionalspheroidsofmesenchymalstemcellsfromsdratspromoteperipheralnerveregeneration AT songyangliu chitinnerveconduitswiththreedimensionalspheroidsofmesenchymalstemcellsfromsdratspromoteperipheralnerveregeneration AT fengshizhang chitinnerveconduitswiththreedimensionalspheroidsofmesenchymalstemcellsfromsdratspromoteperipheralnerveregeneration AT tengwan chitinnerveconduitswiththreedimensionalspheroidsofmesenchymalstemcellsfromsdratspromoteperipheralnerveregeneration AT zhentaoding chitinnerveconduitswiththreedimensionalspheroidsofmesenchymalstemcellsfromsdratspromoteperipheralnerveregeneration AT peixunzhang chitinnerveconduitswiththreedimensionalspheroidsofmesenchymalstemcellsfromsdratspromoteperipheralnerveregeneration
_version_	1718410646552313856

Chitin Nerve Conduits with Three-Dimensional Spheroids of Mesenchymal Stem Cells from SD Rats Promote Peripheral Nerve Regeneration

Ejemplares similares