Lower p66Shc promoter methylation in subjects with chronic renal failure.

<h4>Objective</h4>To determine the correlation between DNA methylation of p66Shc promoter and some markers of inflammatory and oxidative stress in chronic renal failure (CRF) patients compared with healthy subjects.<h4>Methods</h4>An observational cross-sectional study was co...

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Autores principales:	Radhia Hamdi, Amana Saadallah-Kallel, Slima Ferchichi-Trimeche, Raja Mokdad-Gargouri, Abdelhedi Miled, Bachir Benarba
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/515287d6cfa64f9cbf2f5aae7f2f5373
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id	oai:doaj.org-article:515287d6cfa64f9cbf2f5aae7f2f5373
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spelling	oai:doaj.org-article:515287d6cfa64f9cbf2f5aae7f2f53732021-12-02T20:08:13ZLower p66Shc promoter methylation in subjects with chronic renal failure.1932-620310.1371/journal.pone.0257176https://doaj.org/article/515287d6cfa64f9cbf2f5aae7f2f53732021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257176https://doaj.org/toc/1932-6203<h4>Objective</h4>To determine the correlation between DNA methylation of p66Shc promoter and some markers of inflammatory and oxidative stress in chronic renal failure (CRF) patients compared with healthy subjects.<h4>Methods</h4>An observational cross-sectional study was conducted in the nephrology department at Sidi Bouzid Regional Hospital (Tunisia). In total, 39 patients with CRF and 37 healthy subjects were included. Several biochemical parameters were measured. Furthermore, markers of the oxidative and inflammatory status (MDA, TAS, SOD, and CRP) were evaluated. The p66Shc methylation status was determined using the methylation-specific PCR.<h4>Results</h4>Our results showed that levels of blood glucose, urea, creatinine, uric acid, ChT, TG, albuminuria, CRP and MDA were significantly elevated in CRF patients compared to controls. Furthermore, p66Shc promoter region was highly demethylated in CRF patients compared to healthy controls (84% vs 4%). Our data showed a positive correlation between p66Shc hypomethylation and levels of MDA (r = 0.93; p<0, 05) and CRP (r = 0.89; P <0, 05), as well as a significant negative correlation between p66Shc hypomethylation, TAS (r = -0.76; P <0, 05) and SOD (r = -0.77; p<0, 05) levels. Similarly, there was a positive correlation between p66Shc hypomethylation and the disease stages. Importantly, multiple regression analysis showed that p66shc DNA hypomethylation remains strongly correlated with MDA, CRP and stages of CRF.<h4>Conclusion</h4>This study indicates that the DNA hypomethylation of p66shc promoter was correlated with oxidative and inflammatory stress and the disease stages in CRF patients.Radhia HamdiAmana Saadallah-KallelSlima Ferchichi-TrimecheRaja Mokdad-GargouriAbdelhedi MiledBachir BenarbaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257176 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Radhia Hamdi Amana Saadallah-Kallel Slima Ferchichi-Trimeche Raja Mokdad-Gargouri Abdelhedi Miled Bachir Benarba Lower p66Shc promoter methylation in subjects with chronic renal failure.
description	<h4>Objective</h4>To determine the correlation between DNA methylation of p66Shc promoter and some markers of inflammatory and oxidative stress in chronic renal failure (CRF) patients compared with healthy subjects.<h4>Methods</h4>An observational cross-sectional study was conducted in the nephrology department at Sidi Bouzid Regional Hospital (Tunisia). In total, 39 patients with CRF and 37 healthy subjects were included. Several biochemical parameters were measured. Furthermore, markers of the oxidative and inflammatory status (MDA, TAS, SOD, and CRP) were evaluated. The p66Shc methylation status was determined using the methylation-specific PCR.<h4>Results</h4>Our results showed that levels of blood glucose, urea, creatinine, uric acid, ChT, TG, albuminuria, CRP and MDA were significantly elevated in CRF patients compared to controls. Furthermore, p66Shc promoter region was highly demethylated in CRF patients compared to healthy controls (84% vs 4%). Our data showed a positive correlation between p66Shc hypomethylation and levels of MDA (r = 0.93; p<0, 05) and CRP (r = 0.89; P <0, 05), as well as a significant negative correlation between p66Shc hypomethylation, TAS (r = -0.76; P <0, 05) and SOD (r = -0.77; p<0, 05) levels. Similarly, there was a positive correlation between p66Shc hypomethylation and the disease stages. Importantly, multiple regression analysis showed that p66shc DNA hypomethylation remains strongly correlated with MDA, CRP and stages of CRF.<h4>Conclusion</h4>This study indicates that the DNA hypomethylation of p66shc promoter was correlated with oxidative and inflammatory stress and the disease stages in CRF patients.
format	article
author	Radhia Hamdi Amana Saadallah-Kallel Slima Ferchichi-Trimeche Raja Mokdad-Gargouri Abdelhedi Miled Bachir Benarba
author_facet	Radhia Hamdi Amana Saadallah-Kallel Slima Ferchichi-Trimeche Raja Mokdad-Gargouri Abdelhedi Miled Bachir Benarba
author_sort	Radhia Hamdi
title	Lower p66Shc promoter methylation in subjects with chronic renal failure.
title_short	Lower p66Shc promoter methylation in subjects with chronic renal failure.
title_full	Lower p66Shc promoter methylation in subjects with chronic renal failure.
title_fullStr	Lower p66Shc promoter methylation in subjects with chronic renal failure.
title_full_unstemmed	Lower p66Shc promoter methylation in subjects with chronic renal failure.
title_sort	lower p66shc promoter methylation in subjects with chronic renal failure.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/515287d6cfa64f9cbf2f5aae7f2f5373
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Lower p66Shc promoter methylation in subjects with chronic renal failure.

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