Glomerular galactose-deficient IgA1 expression analysis in pediatric patients with glomerular diseases
Abstract Galactose-deficient IgA1 (Gd-IgA1) is important in the pathogenesis of IgA nephropathy (IgAN). A Gd-IgA1-specific monoclonal antibody (KM55) has revealed glomerular Gd-IgA1 deposition solely in patients with IgAN and IgA vasculitis with nephritis (IgAV-N). However, this specificity is contr...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/51582829b5bb434c960fdaccff19cb9f |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:51582829b5bb434c960fdaccff19cb9f |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:51582829b5bb434c960fdaccff19cb9f2021-12-02T16:46:33ZGlomerular galactose-deficient IgA1 expression analysis in pediatric patients with glomerular diseases10.1038/s41598-020-71101-y2045-2322https://doaj.org/article/51582829b5bb434c960fdaccff19cb9f2020-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-71101-yhttps://doaj.org/toc/2045-2322Abstract Galactose-deficient IgA1 (Gd-IgA1) is important in the pathogenesis of IgA nephropathy (IgAN). A Gd-IgA1-specific monoclonal antibody (KM55) has revealed glomerular Gd-IgA1 deposition solely in patients with IgAN and IgA vasculitis with nephritis (IgAV-N). However, this specificity is controversial and has not been demonstrated in pediatric patients. Here, we conducted double-immunofluorescence staining of IgA and Gd-IgA1 in 60 pediatric patients with various glomerular diseases. We divided patients into four groups: (1) patients with IgAN and IgAV-N (n = 23); (2) patients with immunocomplex-mediated glomerulonephritis accompanied by IgA deposition, including lupus nephritis, membranoproliferative glomerulonephritis, and membranous nephropathy (n = 14); (3) patients with other glomerular diseases involving IgA deposition, including idiopathic nephrotic syndrome (INS), oligomeganephronia, Alport syndrome, dense deposit disease, and crescentic glomerulonephritis (n = 11); and (4) patients with IgA-negative diseases including INS, membranoproliferative glomerulonephritis, membranous nephropathy, oligomeganephronia, Alport syndrome, C3 glomerulonephritis, poststreptococcal acute glomerulonephritis, and hemolytic uremic syndrome (n = 12). KM55 staining revealed Gd-IgA1-positive findings in 23/23 patients in Group 1 and 13/14 patients in Group 2, but not in patients in Groups 3 or 4. Therefore, KM55 may detect incidental IgA deposition in pediatric patients. Gd-IgA1 may be involved in the pathogenesis of these immune-related diseases; alternatively, KM55 may recognize IgA-related immunocomplexes in a non-specific manner.Shinya IshikoTomoko HorinouchiRika FujimaruYuko ShimaHiroshi KaitoRyojiro TanakaShingo IshimoriAtsushi KondoSadayuki NagaiYuya AotoNana SakakibaraChina NaganoTomohiko YamamuraMomoka YoshimuraKoichi NakanishiJunya FujimuraNaohiro KamiyoshiHiroaki NagaseNorishige YoshikawaKazumoto IijimaKandai NozuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-6 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Shinya Ishiko Tomoko Horinouchi Rika Fujimaru Yuko Shima Hiroshi Kaito Ryojiro Tanaka Shingo Ishimori Atsushi Kondo Sadayuki Nagai Yuya Aoto Nana Sakakibara China Nagano Tomohiko Yamamura Momoka Yoshimura Koichi Nakanishi Junya Fujimura Naohiro Kamiyoshi Hiroaki Nagase Norishige Yoshikawa Kazumoto Iijima Kandai Nozu Glomerular galactose-deficient IgA1 expression analysis in pediatric patients with glomerular diseases |
| description |
Abstract Galactose-deficient IgA1 (Gd-IgA1) is important in the pathogenesis of IgA nephropathy (IgAN). A Gd-IgA1-specific monoclonal antibody (KM55) has revealed glomerular Gd-IgA1 deposition solely in patients with IgAN and IgA vasculitis with nephritis (IgAV-N). However, this specificity is controversial and has not been demonstrated in pediatric patients. Here, we conducted double-immunofluorescence staining of IgA and Gd-IgA1 in 60 pediatric patients with various glomerular diseases. We divided patients into four groups: (1) patients with IgAN and IgAV-N (n = 23); (2) patients with immunocomplex-mediated glomerulonephritis accompanied by IgA deposition, including lupus nephritis, membranoproliferative glomerulonephritis, and membranous nephropathy (n = 14); (3) patients with other glomerular diseases involving IgA deposition, including idiopathic nephrotic syndrome (INS), oligomeganephronia, Alport syndrome, dense deposit disease, and crescentic glomerulonephritis (n = 11); and (4) patients with IgA-negative diseases including INS, membranoproliferative glomerulonephritis, membranous nephropathy, oligomeganephronia, Alport syndrome, C3 glomerulonephritis, poststreptococcal acute glomerulonephritis, and hemolytic uremic syndrome (n = 12). KM55 staining revealed Gd-IgA1-positive findings in 23/23 patients in Group 1 and 13/14 patients in Group 2, but not in patients in Groups 3 or 4. Therefore, KM55 may detect incidental IgA deposition in pediatric patients. Gd-IgA1 may be involved in the pathogenesis of these immune-related diseases; alternatively, KM55 may recognize IgA-related immunocomplexes in a non-specific manner. |
| format |
article |
| author |
Shinya Ishiko Tomoko Horinouchi Rika Fujimaru Yuko Shima Hiroshi Kaito Ryojiro Tanaka Shingo Ishimori Atsushi Kondo Sadayuki Nagai Yuya Aoto Nana Sakakibara China Nagano Tomohiko Yamamura Momoka Yoshimura Koichi Nakanishi Junya Fujimura Naohiro Kamiyoshi Hiroaki Nagase Norishige Yoshikawa Kazumoto Iijima Kandai Nozu |
| author_facet |
Shinya Ishiko Tomoko Horinouchi Rika Fujimaru Yuko Shima Hiroshi Kaito Ryojiro Tanaka Shingo Ishimori Atsushi Kondo Sadayuki Nagai Yuya Aoto Nana Sakakibara China Nagano Tomohiko Yamamura Momoka Yoshimura Koichi Nakanishi Junya Fujimura Naohiro Kamiyoshi Hiroaki Nagase Norishige Yoshikawa Kazumoto Iijima Kandai Nozu |
| author_sort |
Shinya Ishiko |
| title |
Glomerular galactose-deficient IgA1 expression analysis in pediatric patients with glomerular diseases |
| title_short |
Glomerular galactose-deficient IgA1 expression analysis in pediatric patients with glomerular diseases |
| title_full |
Glomerular galactose-deficient IgA1 expression analysis in pediatric patients with glomerular diseases |
| title_fullStr |
Glomerular galactose-deficient IgA1 expression analysis in pediatric patients with glomerular diseases |
| title_full_unstemmed |
Glomerular galactose-deficient IgA1 expression analysis in pediatric patients with glomerular diseases |
| title_sort |
glomerular galactose-deficient iga1 expression analysis in pediatric patients with glomerular diseases |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/51582829b5bb434c960fdaccff19cb9f |
| work_keys_str_mv |
AT shinyaishiko glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT tomokohorinouchi glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT rikafujimaru glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT yukoshima glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT hiroshikaito glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT ryojirotanaka glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT shingoishimori glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT atsushikondo glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT sadayukinagai glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT yuyaaoto glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT nanasakakibara glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT chinanagano glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT tomohikoyamamura glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT momokayoshimura glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT koichinakanishi glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT junyafujimura glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT naohirokamiyoshi glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT hiroakinagase glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT norishigeyoshikawa glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT kazumotoiijima glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases AT kandainozu glomerulargalactosedeficientiga1expressionanalysisinpediatricpatientswithglomerulardiseases |
| _version_ |
1718383395785932800 |