Nanostructured polyurethane-poly-lactic- co-glycolic acid scaffolds increase bladder tissue regeneration: an in vivo study

Chang Yao,1 Matt Hedrick,1 Gyan Pareek,2 Joseph Renzulli,2 George Haleblian,2 Thomas J Webster3 1Nanovis LLC, West Lafayette, IN, 2Section of Minimally Invasive Urology, Alpert Medical School, Brown University, Providence, RI, 3Department of Chemical Engineering and Program in Bioengineering, Northe...

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Autores principales:	Yao C, Hedrick M, Pareek G, Renzulli J, Haleblian G, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/515a5dac222746ffb8d1e6a331ef5285
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spelling	oai:doaj.org-article:515a5dac222746ffb8d1e6a331ef52852021-12-02T00:35:30ZNanostructured polyurethane-poly-lactic- co-glycolic acid scaffolds increase bladder tissue regeneration: an in vivo study1176-91141178-2013https://doaj.org/article/515a5dac222746ffb8d1e6a331ef52852013-08-01T00:00:00Zhttp://www.dovepress.com/nanostructured-polyurethane-poly-lactic--co-glycolic-acid-scaffolds-in-a14192https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Chang Yao,1 Matt Hedrick,1 Gyan Pareek,2 Joseph Renzulli,2 George Haleblian,2 Thomas J Webster3 1Nanovis LLC, West Lafayette, IN, 2Section of Minimally Invasive Urology, Alpert Medical School, Brown University, Providence, RI, 3Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA Abstract: Although showing much promise for numerous tissue engineering applications, polyurethane and poly-lactic-co-glycolic acid (PLGA) have suffered from a lack of cytocompatibility, sometimes leading to poor tissue integration. Nanotechnology (or the use of materials with surface features or constituent dimensions less than 100 nm in at least one direction) has started to transform currently implanted materials (such as polyurethane and PLGA) to promote tissue regeneration. This is because nanostructured surface features can be used to change medical device surface energy to alter initial protein adsorption events important for promoting tissue-forming cell functions. Thus, due to their altered surface energetics, the objective of the present in vivo study was to create nanoscale surface features on a new polyurethane and PLGA composite scaffold (by soaking the polyurethane side and PLGA side in HNO3 and NaOH, respectively) and determine bladder tissue regeneration using a minipig model. The novel nanostructured scaffolds were further functionalized with IKVAV and YIGSR peptides to improve cellular responses. Results provided the first evidence of increased in vivo bladder tissue regeneration when using a composite of nanostructured polyurethane and PLGA compared with control ileal segments. Due to additional surgery, extended potentially problematic healing times, metabolic complications, donor site morbidity, and sometimes limited availability, ileal segment repair of a bladder defect is not optimal and, thus, a synthetic analog is highly desirable. In summary, this study indicates significant promise for the use of nanostructured polyurethane and PLGA composites to increase bladder tissue repair for a wide range of regenerative medicine applications, such as regenerating bladder tissue after removal of cancerous tissue, disease, or other trauma. Keywords: polyurethane, poly-lactic-co-glycolic acid, nanotechnology, nanostructured features, in vivoYao CHedrick MPareek GRenzulli JHaleblian GWebster TJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 3285-3296 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yao C Hedrick M Pareek G Renzulli J Haleblian G Webster TJ Nanostructured polyurethane-poly-lactic- co-glycolic acid scaffolds increase bladder tissue regeneration: an in vivo study
description	Chang Yao,1 Matt Hedrick,1 Gyan Pareek,2 Joseph Renzulli,2 George Haleblian,2 Thomas J Webster3 1Nanovis LLC, West Lafayette, IN, 2Section of Minimally Invasive Urology, Alpert Medical School, Brown University, Providence, RI, 3Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA Abstract: Although showing much promise for numerous tissue engineering applications, polyurethane and poly-lactic-co-glycolic acid (PLGA) have suffered from a lack of cytocompatibility, sometimes leading to poor tissue integration. Nanotechnology (or the use of materials with surface features or constituent dimensions less than 100 nm in at least one direction) has started to transform currently implanted materials (such as polyurethane and PLGA) to promote tissue regeneration. This is because nanostructured surface features can be used to change medical device surface energy to alter initial protein adsorption events important for promoting tissue-forming cell functions. Thus, due to their altered surface energetics, the objective of the present in vivo study was to create nanoscale surface features on a new polyurethane and PLGA composite scaffold (by soaking the polyurethane side and PLGA side in HNO3 and NaOH, respectively) and determine bladder tissue regeneration using a minipig model. The novel nanostructured scaffolds were further functionalized with IKVAV and YIGSR peptides to improve cellular responses. Results provided the first evidence of increased in vivo bladder tissue regeneration when using a composite of nanostructured polyurethane and PLGA compared with control ileal segments. Due to additional surgery, extended potentially problematic healing times, metabolic complications, donor site morbidity, and sometimes limited availability, ileal segment repair of a bladder defect is not optimal and, thus, a synthetic analog is highly desirable. In summary, this study indicates significant promise for the use of nanostructured polyurethane and PLGA composites to increase bladder tissue repair for a wide range of regenerative medicine applications, such as regenerating bladder tissue after removal of cancerous tissue, disease, or other trauma. Keywords: polyurethane, poly-lactic-co-glycolic acid, nanotechnology, nanostructured features, in vivo
format	article
author	Yao C Hedrick M Pareek G Renzulli J Haleblian G Webster TJ
author_facet	Yao C Hedrick M Pareek G Renzulli J Haleblian G Webster TJ
author_sort	Yao C
title	Nanostructured polyurethane-poly-lactic- co-glycolic acid scaffolds increase bladder tissue regeneration: an in vivo study
title_short	Nanostructured polyurethane-poly-lactic- co-glycolic acid scaffolds increase bladder tissue regeneration: an in vivo study
title_full	Nanostructured polyurethane-poly-lactic- co-glycolic acid scaffolds increase bladder tissue regeneration: an in vivo study
title_fullStr	Nanostructured polyurethane-poly-lactic- co-glycolic acid scaffolds increase bladder tissue regeneration: an in vivo study
title_full_unstemmed	Nanostructured polyurethane-poly-lactic- co-glycolic acid scaffolds increase bladder tissue regeneration: an in vivo study
title_sort	nanostructured polyurethane-poly-lactic- co-glycolic acid scaffolds increase bladder tissue regeneration: an in vivo study
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/515a5dac222746ffb8d1e6a331ef5285
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Nanostructured polyurethane-poly-lactic- co-glycolic acid scaffolds increase bladder tissue regeneration: an in vivo study

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