Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications

Abstract Healthy plants are vital for successful, long-duration missions in space, as they provide the crew with life support, food production, and psychological benefits. The microorganisms that associate with plant tissues play a critical role in improving plant health and production. To that end,...

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Autores principales:	Natasha J. Haveman, Christina L. M. Khodadad, Anirudha R. Dixit, Artemis S. Louyakis, Gioia D. Massa, Kasthuri Venkateswaran, Jamie S. Foster
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biotechnology TP248.13-248.65 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/515d08e1c1f648f68e3fa4230585a1ae
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id	oai:doaj.org-article:515d08e1c1f648f68e3fa4230585a1ae
record_format	dspace
spelling	oai:doaj.org-article:515d08e1c1f648f68e3fa4230585a1ae2021-12-02T17:40:43ZEvaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications10.1038/s41526-021-00151-x2373-8065https://doaj.org/article/515d08e1c1f648f68e3fa4230585a1ae2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41526-021-00151-xhttps://doaj.org/toc/2373-8065Abstract Healthy plants are vital for successful, long-duration missions in space, as they provide the crew with life support, food production, and psychological benefits. The microorganisms that associate with plant tissues play a critical role in improving plant health and production. To that end, we developed a methodology to investigate the transcriptional activities of the microbiome of red romaine lettuce, a key salad crop that was grown under International Space Station (ISS)-like conditions. Microbial transcripts enriched from host–microbe total RNA were sequenced using the Oxford Nanopore MinION sequencing platform. Results show that this enrichment approach was highly reproducible and could be an effective approach for the on-site detection of microbial transcriptional activity. Our results demonstrate the feasibility of using metatranscriptomics of enriched microbial RNA as a potential method for on-site monitoring of the transcriptional activity of crop microbiomes, thereby helping to facilitate and maintain plant health for on-orbit space food production.Natasha J. HavemanChristina L. M. KhodadadAnirudha R. DixitArtemis S. LouyakisGioia D. MassaKasthuri VenkateswaranJamie S. FosterNature PortfolioarticleBiotechnologyTP248.13-248.65PhysiologyQP1-981ENnpj Microgravity, Vol 7, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65 Physiology QP1-981
spellingShingle	Biotechnology TP248.13-248.65 Physiology QP1-981 Natasha J. Haveman Christina L. M. Khodadad Anirudha R. Dixit Artemis S. Louyakis Gioia D. Massa Kasthuri Venkateswaran Jamie S. Foster Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications
description	Abstract Healthy plants are vital for successful, long-duration missions in space, as they provide the crew with life support, food production, and psychological benefits. The microorganisms that associate with plant tissues play a critical role in improving plant health and production. To that end, we developed a methodology to investigate the transcriptional activities of the microbiome of red romaine lettuce, a key salad crop that was grown under International Space Station (ISS)-like conditions. Microbial transcripts enriched from host–microbe total RNA were sequenced using the Oxford Nanopore MinION sequencing platform. Results show that this enrichment approach was highly reproducible and could be an effective approach for the on-site detection of microbial transcriptional activity. Our results demonstrate the feasibility of using metatranscriptomics of enriched microbial RNA as a potential method for on-site monitoring of the transcriptional activity of crop microbiomes, thereby helping to facilitate and maintain plant health for on-orbit space food production.
format	article
author	Natasha J. Haveman Christina L. M. Khodadad Anirudha R. Dixit Artemis S. Louyakis Gioia D. Massa Kasthuri Venkateswaran Jamie S. Foster
author_facet	Natasha J. Haveman Christina L. M. Khodadad Anirudha R. Dixit Artemis S. Louyakis Gioia D. Massa Kasthuri Venkateswaran Jamie S. Foster
author_sort	Natasha J. Haveman
title	Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications
title_short	Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications
title_full	Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications
title_fullStr	Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications
title_full_unstemmed	Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications
title_sort	evaluating the lettuce metatranscriptome with minion sequencing for future spaceflight food production applications
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/515d08e1c1f648f68e3fa4230585a1ae
work_keys_str_mv	AT natashajhaveman evaluatingthelettucemetatranscriptomewithminionsequencingforfuturespaceflightfoodproductionapplications AT christinalmkhodadad evaluatingthelettucemetatranscriptomewithminionsequencingforfuturespaceflightfoodproductionapplications AT anirudhardixit evaluatingthelettucemetatranscriptomewithminionsequencingforfuturespaceflightfoodproductionapplications AT artemisslouyakis evaluatingthelettucemetatranscriptomewithminionsequencingforfuturespaceflightfoodproductionapplications AT gioiadmassa evaluatingthelettucemetatranscriptomewithminionsequencingforfuturespaceflightfoodproductionapplications AT kasthurivenkateswaran evaluatingthelettucemetatranscriptomewithminionsequencingforfuturespaceflightfoodproductionapplications AT jamiesfoster evaluatingthelettucemetatranscriptomewithminionsequencingforfuturespaceflightfoodproductionapplications
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Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications

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