Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis

Abstract Ruminal microbiota changes frequently with high grain diets and the occurrence of subacute ruminal acidosis (SARA). A grain-induced goat model of SARA, with durations of a significant decrease in the rumen pH value to less than 5.6 and an increase in the rumen lipopolysaccharides concentrat...

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Autores principales:	Xiaodong Chen, Xiaodong Su, Jilong Li, Yuntian Yang, Peiyue Wang, Fang Yan, Junhu Yao, Shengru Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/515d665099074f6686211d0939376990
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spelling	oai:doaj.org-article:515d665099074f6686211d09393769902021-12-02T17:15:22ZReal-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis10.1038/s41522-021-00215-62055-5008https://doaj.org/article/515d665099074f6686211d09393769902021-05-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00215-6https://doaj.org/toc/2055-5008Abstract Ruminal microbiota changes frequently with high grain diets and the occurrence of subacute ruminal acidosis (SARA). A grain-induced goat model of SARA, with durations of a significant decrease in the rumen pH value to less than 5.6 and an increase in the rumen lipopolysaccharides concentration, is constructed for real-time monitoring of bacteria alteration. Using 16 S rRNA gene sequencing, significant bacterial differences between goats from the SARA and healthy groups are identified at every hour for six continuous hours after feeding. Moreover, 29 common differential genera between two groups over 6 h after feeding are all related to the altered pH and lipopolysaccharides. Transplanting the microbiota from donor goats with SARA could induce colonic inflammation in antibiotic-pretreated mice. Overall, significant differences in the bacterial community and rumen fermentation pattern between the healthy and SARA dairy goats are real-time monitored, and then tested using ruminal microbe transplantation to antibiotic-treated mice.Xiaodong ChenXiaodong SuJilong LiYuntian YangPeiyue WangFang YanJunhu YaoShengru WuNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Xiaodong Chen Xiaodong Su Jilong Li Yuntian Yang Peiyue Wang Fang Yan Junhu Yao Shengru Wu Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis
description	Abstract Ruminal microbiota changes frequently with high grain diets and the occurrence of subacute ruminal acidosis (SARA). A grain-induced goat model of SARA, with durations of a significant decrease in the rumen pH value to less than 5.6 and an increase in the rumen lipopolysaccharides concentration, is constructed for real-time monitoring of bacteria alteration. Using 16 S rRNA gene sequencing, significant bacterial differences between goats from the SARA and healthy groups are identified at every hour for six continuous hours after feeding. Moreover, 29 common differential genera between two groups over 6 h after feeding are all related to the altered pH and lipopolysaccharides. Transplanting the microbiota from donor goats with SARA could induce colonic inflammation in antibiotic-pretreated mice. Overall, significant differences in the bacterial community and rumen fermentation pattern between the healthy and SARA dairy goats are real-time monitored, and then tested using ruminal microbe transplantation to antibiotic-treated mice.
format	article
author	Xiaodong Chen Xiaodong Su Jilong Li Yuntian Yang Peiyue Wang Fang Yan Junhu Yao Shengru Wu
author_facet	Xiaodong Chen Xiaodong Su Jilong Li Yuntian Yang Peiyue Wang Fang Yan Junhu Yao Shengru Wu
author_sort	Xiaodong Chen
title	Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis
title_short	Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis
title_full	Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis
title_fullStr	Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis
title_full_unstemmed	Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis
title_sort	real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/515d665099074f6686211d0939376990
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Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis

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