Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland

Abstract The anterior pituitary gland regulates growth, metabolism, and reproduction by secreting hormones. Folliculo-stellate (FS) cells are non-endocrine cells located among hormone-producing cells in the anterior pituitary glands. They form follicular lumens, which are sealed by tight junctions (...

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Autores principales:	Atsuko Y. Higashi, Tomohito Higashi, Kyoko Furuse, Kana Ozeki, Mikio Furuse, Hideki Chiba
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5168da6e5be84973bd8b68a78fcf4d0d
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spelling	oai:doaj.org-article:5168da6e5be84973bd8b68a78fcf4d0d2021-11-08T10:48:53ZClaudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland10.1038/s41598-021-01004-z2045-2322https://doaj.org/article/5168da6e5be84973bd8b68a78fcf4d0d2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01004-zhttps://doaj.org/toc/2045-2322Abstract The anterior pituitary gland regulates growth, metabolism, and reproduction by secreting hormones. Folliculo-stellate (FS) cells are non-endocrine cells located among hormone-producing cells in the anterior pituitary glands. They form follicular lumens, which are sealed by tight junctions (TJs). Although FS cells are hypothesized to contribute to fine-tuning of endocrine cells, little is known about the exact roles of FS cells. Here, we investigated the molecular composition of TJs in FS cells. We demonstrated that occludin is a good marker for TJs in the pituitary gland and examined the structure of the lumens surrounded by FS cells. We also found that claudin-9 is a major component of TJs in the FS cells. In immunoelectron microscopy, claudin-9 was specifically localized at TJs of the FS cells. The expression of claudin-9 was gradually increased in the pituitary gland after birth, suggesting that claudin-9 is developmentally regulated and performs some specific functions on the paracellular barrier of follicles in the pituitary gland. Furthermore, we found that angulin-1, angulin-2, and tricellulin are localized at the tricellular contacts of the FS cells. Our findings provide a first comprehensive molecular profile of TJs in the FS cells, and may lead us towards unveiling the FS cell functions.Atsuko Y. HigashiTomohito HigashiKyoko FuruseKana OzekiMikio FuruseHideki ChibaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Atsuko Y. Higashi Tomohito Higashi Kyoko Furuse Kana Ozeki Mikio Furuse Hideki Chiba Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland
description	Abstract The anterior pituitary gland regulates growth, metabolism, and reproduction by secreting hormones. Folliculo-stellate (FS) cells are non-endocrine cells located among hormone-producing cells in the anterior pituitary glands. They form follicular lumens, which are sealed by tight junctions (TJs). Although FS cells are hypothesized to contribute to fine-tuning of endocrine cells, little is known about the exact roles of FS cells. Here, we investigated the molecular composition of TJs in FS cells. We demonstrated that occludin is a good marker for TJs in the pituitary gland and examined the structure of the lumens surrounded by FS cells. We also found that claudin-9 is a major component of TJs in the FS cells. In immunoelectron microscopy, claudin-9 was specifically localized at TJs of the FS cells. The expression of claudin-9 was gradually increased in the pituitary gland after birth, suggesting that claudin-9 is developmentally regulated and performs some specific functions on the paracellular barrier of follicles in the pituitary gland. Furthermore, we found that angulin-1, angulin-2, and tricellulin are localized at the tricellular contacts of the FS cells. Our findings provide a first comprehensive molecular profile of TJs in the FS cells, and may lead us towards unveiling the FS cell functions.
format	article
author	Atsuko Y. Higashi Tomohito Higashi Kyoko Furuse Kana Ozeki Mikio Furuse Hideki Chiba
author_facet	Atsuko Y. Higashi Tomohito Higashi Kyoko Furuse Kana Ozeki Mikio Furuse Hideki Chiba
author_sort	Atsuko Y. Higashi
title	Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland
title_short	Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland
title_full	Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland
title_fullStr	Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland
title_full_unstemmed	Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland
title_sort	claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5168da6e5be84973bd8b68a78fcf4d0d
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Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland

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