Silent infection of human dendritic cells by African and Asian strains of Zika virus

Abstract While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborn...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nathalie J. Vielle, Beatrice Zumkehr, Obdulio García-Nicolás, Fabian Blank, Miloš Stojanov, Didier Musso, David Baud, Artur Summerfield, Marco P. Alves
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/516bac2ea8f142af83a69f503aab51d6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:516bac2ea8f142af83a69f503aab51d6
record_format	dspace
spelling	oai:doaj.org-article:516bac2ea8f142af83a69f503aab51d62021-12-02T15:07:56ZSilent infection of human dendritic cells by African and Asian strains of Zika virus10.1038/s41598-018-23734-32045-2322https://doaj.org/article/516bac2ea8f142af83a69f503aab51d62018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-23734-3https://doaj.org/toc/2045-2322Abstract While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborns/neonates and adults. With the aim to identify virus lineage-dependent factors, we compared cell susceptibility, virus replication, cell death and innate immune responses following infection with two African and three contemporary Asian lineage strains of ZIKV. To this end, we used green monkey Vero and Aedes albopictus C6/36 cells and human monocyte-derived dendritic cells (DCs). The latter are involved in the pathogenesis of several mosquito-borne Flavivirus infections. In Vero and C6/36 cells, we observed strain- but not lineage-dependent differences in infection profiles. Nevertheless, in human DCs, no significant differences in susceptibility and virus replication were found between lineages and strains. ZIKV induced antiviral interferon type I/III in a limited fashion, with the exception of one African strain. None of the strains induced cell death or DC maturation in terms of MHC II, CD40, CD80/86 or CCR7 expression. Taken together, our data suggest that a large collection of virus isolates needs to be investigated before conclusions on lineage differences can be made.Nathalie J. VielleBeatrice ZumkehrObdulio García-NicolásFabian BlankMiloš StojanovDidier MussoDavid BaudArtur SummerfieldMarco P. AlvesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nathalie J. Vielle Beatrice Zumkehr Obdulio García-Nicolás Fabian Blank Miloš Stojanov Didier Musso David Baud Artur Summerfield Marco P. Alves Silent infection of human dendritic cells by African and Asian strains of Zika virus
description	Abstract While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborns/neonates and adults. With the aim to identify virus lineage-dependent factors, we compared cell susceptibility, virus replication, cell death and innate immune responses following infection with two African and three contemporary Asian lineage strains of ZIKV. To this end, we used green monkey Vero and Aedes albopictus C6/36 cells and human monocyte-derived dendritic cells (DCs). The latter are involved in the pathogenesis of several mosquito-borne Flavivirus infections. In Vero and C6/36 cells, we observed strain- but not lineage-dependent differences in infection profiles. Nevertheless, in human DCs, no significant differences in susceptibility and virus replication were found between lineages and strains. ZIKV induced antiviral interferon type I/III in a limited fashion, with the exception of one African strain. None of the strains induced cell death or DC maturation in terms of MHC II, CD40, CD80/86 or CCR7 expression. Taken together, our data suggest that a large collection of virus isolates needs to be investigated before conclusions on lineage differences can be made.
format	article
author	Nathalie J. Vielle Beatrice Zumkehr Obdulio García-Nicolás Fabian Blank Miloš Stojanov Didier Musso David Baud Artur Summerfield Marco P. Alves
author_facet	Nathalie J. Vielle Beatrice Zumkehr Obdulio García-Nicolás Fabian Blank Miloš Stojanov Didier Musso David Baud Artur Summerfield Marco P. Alves
author_sort	Nathalie J. Vielle
title	Silent infection of human dendritic cells by African and Asian strains of Zika virus
title_short	Silent infection of human dendritic cells by African and Asian strains of Zika virus
title_full	Silent infection of human dendritic cells by African and Asian strains of Zika virus
title_fullStr	Silent infection of human dendritic cells by African and Asian strains of Zika virus
title_full_unstemmed	Silent infection of human dendritic cells by African and Asian strains of Zika virus
title_sort	silent infection of human dendritic cells by african and asian strains of zika virus
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/516bac2ea8f142af83a69f503aab51d6
work_keys_str_mv	AT nathaliejvielle silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus AT beatricezumkehr silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus AT obduliogarcianicolas silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus AT fabianblank silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus AT milosstojanov silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus AT didiermusso silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus AT davidbaud silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus AT artursummerfield silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus AT marcopalves silentinfectionofhumandendriticcellsbyafricanandasianstrainsofzikavirus
_version_	1718388315023998976

Silent infection of human dendritic cells by African and Asian strains of Zika virus

Ejemplares similares