MicroRNA-197-3p Inhibits the Osteogenic Differentiation in Osteoporosis by Down-Regulating KLF 10

MicroRNA-197-3p Inhibits the Osteogenic Differentiation in Osteoporosis by Down-Regulating KLF 10

Murong You, Liang Zhang, Xiaoxiang Zhang, Yang Fu, Xieping Dong Department of Orthopedics, Jiangxi Provincial People’s Hospital, Nanchang, Jiangxi Province 330006, People’s Republic of ChinaCorrespondence: Murong YouDepartment of Orthopedics, Jiangxi Provincial People’s...

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Autores principales: You M, Zhang L, Zhang X, Fu Y, Dong X
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2021
Materias:
osteoporosis
mir-197-3p
klf10
osteogenic differentiation
mc3t3-e1 cells
Geriatrics
RC952-954.6
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spelling oai:doaj.org-article:516daddf646f4dc19aabbaa8fa4127982021-12-02T14:01:49ZMicroRNA-197-3p Inhibits the Osteogenic Differentiation in Osteoporosis by Down-Regulating KLF 101178-1998https://doaj.org/article/516daddf646f4dc19aabbaa8fa4127982021-01-01T00:00:00Zhttps://www.dovepress.com/microrna-197-3p-inhibits-the-osteogenic-differentiation-in-osteoporosi-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Murong You, Liang Zhang, Xiaoxiang Zhang, Yang Fu, Xieping Dong Department of Orthopedics, Jiangxi Provincial People’s Hospital, Nanchang, Jiangxi Province 330006, People’s Republic of ChinaCorrespondence: Murong YouDepartment of Orthopedics, Jiangxi Provincial People’s Hospital, Nanchang, Jiangxi Province 330006, People’s Republic of ChinaEmail mr1058@163.comBackground: Studies have shown that microRNA (miRNA) regulates gene expression of osteoporosis (OS). It is known that miR-197-3p is abnormally expressed in osteoporosis. This study is to investigate the mechanism of miR-197-3p in regulating osteoblast differentiation.Methods: Rats were ovariectomized to establish an animal model of postmenopausal osteoporosis. The expression of miR-197-3p and KLF10 was detected in ovariectomized rat models. Primary osteoblasts and MC3T-E1 cells were divided into the control group, miR-197-3p inhibitor group, NC inhibitor group and miR-197-3p inhibitor + si-KLF10 group. The expression of miR-197-3p and Kruppel-like factor 10 (KLF10) was detected by qRT-PCR and Western blot. The relationship between miR-197-3p and KLF10 was analyzed by bioinformatics and luciferase reporter assay. Cell viability was evaluated by MTT assay. The ALP activity measurement and mineralization analysis were performed.Results: The expression of miR-197-3p was significantly raised in ovariectomized osteoporosis rats. During the differentiation of osteoblasts, the expression of miR-197-3p was significantly decreased, while the expression of KLF10 was significantly raised in primary osteoblasts and MC3E3T1 cells. The expression of RUNX2, ALP, OCN and OSX in miR-197-3p inhibitor group and MC3T3-E1 group was significantly raised, and the cell survival rate and mineralized nodule were raised as well. KLF10 may be the downstream target gene of miR-197-3p. After co-transfection of miR-197-3p inhibitor and si-klf10, ALP, Runx2, OCN and OSX mRNA, cell survival rate and mineralized nodule were significantly decreased in primary osteoblasts and MC3T3-E1 cells.Conclusion: MiR-197-3p Inhibition promoted osteoblast differentiation and reduced OS by up-regulating KLF10.Keywords: osteoporosis, miR-197-3p, KLF10, osteogenic differentiation, MC3T3-E1 cellsYou MZhang LZhang XFu YDong XDove Medical Pressarticleosteoporosismir-197-3pklf10osteogenic differentiationmc3t3-e1 cellsGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 16, Pp 107-117 (2021)
institution DOAJ
collection DOAJ
language EN
topic osteoporosis
mir-197-3p
klf10
osteogenic differentiation
mc3t3-e1 cells
Geriatrics
RC952-954.6
spellingShingle osteoporosis
mir-197-3p
klf10
osteogenic differentiation
mc3t3-e1 cells
Geriatrics
RC952-954.6
You M
Zhang L
Zhang X
Fu Y
Dong X
MicroRNA-197-3p Inhibits the Osteogenic Differentiation in Osteoporosis by Down-Regulating KLF 10
description Murong You, Liang Zhang, Xiaoxiang Zhang, Yang Fu, Xieping Dong Department of Orthopedics, Jiangxi Provincial People’s Hospital, Nanchang, Jiangxi Province 330006, People’s Republic of ChinaCorrespondence: Murong YouDepartment of Orthopedics, Jiangxi Provincial People’s Hospital, Nanchang, Jiangxi Province 330006, People’s Republic of ChinaEmail mr1058@163.comBackground: Studies have shown that microRNA (miRNA) regulates gene expression of osteoporosis (OS). It is known that miR-197-3p is abnormally expressed in osteoporosis. This study is to investigate the mechanism of miR-197-3p in regulating osteoblast differentiation.Methods: Rats were ovariectomized to establish an animal model of postmenopausal osteoporosis. The expression of miR-197-3p and KLF10 was detected in ovariectomized rat models. Primary osteoblasts and MC3T-E1 cells were divided into the control group, miR-197-3p inhibitor group, NC inhibitor group and miR-197-3p inhibitor + si-KLF10 group. The expression of miR-197-3p and Kruppel-like factor 10 (KLF10) was detected by qRT-PCR and Western blot. The relationship between miR-197-3p and KLF10 was analyzed by bioinformatics and luciferase reporter assay. Cell viability was evaluated by MTT assay. The ALP activity measurement and mineralization analysis were performed.Results: The expression of miR-197-3p was significantly raised in ovariectomized osteoporosis rats. During the differentiation of osteoblasts, the expression of miR-197-3p was significantly decreased, while the expression of KLF10 was significantly raised in primary osteoblasts and MC3E3T1 cells. The expression of RUNX2, ALP, OCN and OSX in miR-197-3p inhibitor group and MC3T3-E1 group was significantly raised, and the cell survival rate and mineralized nodule were raised as well. KLF10 may be the downstream target gene of miR-197-3p. After co-transfection of miR-197-3p inhibitor and si-klf10, ALP, Runx2, OCN and OSX mRNA, cell survival rate and mineralized nodule were significantly decreased in primary osteoblasts and MC3T3-E1 cells.Conclusion: MiR-197-3p Inhibition promoted osteoblast differentiation and reduced OS by up-regulating KLF10.Keywords: osteoporosis, miR-197-3p, KLF10, osteogenic differentiation, MC3T3-E1 cells
format article
author You M
Zhang L
Zhang X
Fu Y
Dong X
author_facet You M
Zhang L
Zhang X
Fu Y
Dong X
author_sort You M
title MicroRNA-197-3p Inhibits the Osteogenic Differentiation in Osteoporosis by Down-Regulating KLF 10
title_short MicroRNA-197-3p Inhibits the Osteogenic Differentiation in Osteoporosis by Down-Regulating KLF 10
title_full MicroRNA-197-3p Inhibits the Osteogenic Differentiation in Osteoporosis by Down-Regulating KLF 10
title_fullStr MicroRNA-197-3p Inhibits the Osteogenic Differentiation in Osteoporosis by Down-Regulating KLF 10
title_full_unstemmed MicroRNA-197-3p Inhibits the Osteogenic Differentiation in Osteoporosis by Down-Regulating KLF 10
title_sort microrna-197-3p inhibits the osteogenic differentiation in osteoporosis by down-regulating klf 10
publisher Dove Medical Press
publishDate 2021
url https://doaj.org/article/516daddf646f4dc19aabbaa8fa412798
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AT zhangx microrna1973pinhibitstheosteogenicdifferentiationinosteoporosisbydownregulatingklf10
AT fuy microrna1973pinhibitstheosteogenicdifferentiationinosteoporosisbydownregulatingklf10
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