Intranasal inoculation of white-tailed deer (Odocoileus virginianus) with lyophilized chronic wasting disease prion particulate complexed to montmorillonite clay.

Chronic wasting disease (CWD), the only known prion disease endemic in wildlife, is a persistent problem in both wild and captive North American cervid populations. This disease continues to spread and cases are found in new areas each year. Indirect transmission can occur via the environment and is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tracy A Nichols, Terry R Spraker, Tara D Rigg, Crystal Meyerett-Reid, Clare Hoover, Brady Michel, Jifeng Bian, Edward Hoover, Thomas Gidlewski, Aru Balachandran, Katherine O'Rourke, Glenn C Telling, Richard Bowen, Mark D Zabel, Kurt C VerCauteren
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/519cd09084264ef8ab5eac16275fdefe
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:519cd09084264ef8ab5eac16275fdefe
record_format	dspace
spelling	oai:doaj.org-article:519cd09084264ef8ab5eac16275fdefe2021-11-18T07:46:15ZIntranasal inoculation of white-tailed deer (Odocoileus virginianus) with lyophilized chronic wasting disease prion particulate complexed to montmorillonite clay.1932-620310.1371/journal.pone.0062455https://doaj.org/article/519cd09084264ef8ab5eac16275fdefe2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23671598/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Chronic wasting disease (CWD), the only known prion disease endemic in wildlife, is a persistent problem in both wild and captive North American cervid populations. This disease continues to spread and cases are found in new areas each year. Indirect transmission can occur via the environment and is thought to occur by the oral and/or intranasal route. Oral transmission has been experimentally demonstrated and although intranasal transmission has been postulated, it has not been tested in a natural host until recently. Prions have been shown to adsorb strongly to clay particles and upon oral inoculation the prion/clay combination exhibits increased infectivity in rodent models. Deer and elk undoubtedly and chronically inhale dust particles routinely while living in the landscape while foraging and rutting. We therefore hypothesized that dust represents a viable vehicle for intranasal CWD prion exposure. To test this hypothesis, CWD-positive brain homogenate was mixed with montmorillonite clay (Mte), lyophilized, pulverized and inoculated intranasally into white-tailed deer once a week for 6 weeks. Deer were euthanized at 95, 105, 120 and 175 days post final inoculation and tissues examined for CWD-associated prion proteins by immunohistochemistry. Our results demonstrate that CWD can be efficiently transmitted utilizing Mte particles as a prion carrier and intranasal exposure.Tracy A NicholsTerry R SprakerTara D RiggCrystal Meyerett-ReidClare HooverBrady MichelJifeng BianEdward HooverThomas GidlewskiAru BalachandranKatherine O'RourkeGlenn C TellingRichard BowenMark D ZabelKurt C VerCauterenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e62455 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tracy A Nichols Terry R Spraker Tara D Rigg Crystal Meyerett-Reid Clare Hoover Brady Michel Jifeng Bian Edward Hoover Thomas Gidlewski Aru Balachandran Katherine O'Rourke Glenn C Telling Richard Bowen Mark D Zabel Kurt C VerCauteren Intranasal inoculation of white-tailed deer (Odocoileus virginianus) with lyophilized chronic wasting disease prion particulate complexed to montmorillonite clay.
description	Chronic wasting disease (CWD), the only known prion disease endemic in wildlife, is a persistent problem in both wild and captive North American cervid populations. This disease continues to spread and cases are found in new areas each year. Indirect transmission can occur via the environment and is thought to occur by the oral and/or intranasal route. Oral transmission has been experimentally demonstrated and although intranasal transmission has been postulated, it has not been tested in a natural host until recently. Prions have been shown to adsorb strongly to clay particles and upon oral inoculation the prion/clay combination exhibits increased infectivity in rodent models. Deer and elk undoubtedly and chronically inhale dust particles routinely while living in the landscape while foraging and rutting. We therefore hypothesized that dust represents a viable vehicle for intranasal CWD prion exposure. To test this hypothesis, CWD-positive brain homogenate was mixed with montmorillonite clay (Mte), lyophilized, pulverized and inoculated intranasally into white-tailed deer once a week for 6 weeks. Deer were euthanized at 95, 105, 120 and 175 days post final inoculation and tissues examined for CWD-associated prion proteins by immunohistochemistry. Our results demonstrate that CWD can be efficiently transmitted utilizing Mte particles as a prion carrier and intranasal exposure.
format	article
author	Tracy A Nichols Terry R Spraker Tara D Rigg Crystal Meyerett-Reid Clare Hoover Brady Michel Jifeng Bian Edward Hoover Thomas Gidlewski Aru Balachandran Katherine O'Rourke Glenn C Telling Richard Bowen Mark D Zabel Kurt C VerCauteren
author_facet	Tracy A Nichols Terry R Spraker Tara D Rigg Crystal Meyerett-Reid Clare Hoover Brady Michel Jifeng Bian Edward Hoover Thomas Gidlewski Aru Balachandran Katherine O'Rourke Glenn C Telling Richard Bowen Mark D Zabel Kurt C VerCauteren
author_sort	Tracy A Nichols
title	Intranasal inoculation of white-tailed deer (Odocoileus virginianus) with lyophilized chronic wasting disease prion particulate complexed to montmorillonite clay.
title_short	Intranasal inoculation of white-tailed deer (Odocoileus virginianus) with lyophilized chronic wasting disease prion particulate complexed to montmorillonite clay.
title_full	Intranasal inoculation of white-tailed deer (Odocoileus virginianus) with lyophilized chronic wasting disease prion particulate complexed to montmorillonite clay.
title_fullStr	Intranasal inoculation of white-tailed deer (Odocoileus virginianus) with lyophilized chronic wasting disease prion particulate complexed to montmorillonite clay.
title_full_unstemmed	Intranasal inoculation of white-tailed deer (Odocoileus virginianus) with lyophilized chronic wasting disease prion particulate complexed to montmorillonite clay.
title_sort	intranasal inoculation of white-tailed deer (odocoileus virginianus) with lyophilized chronic wasting disease prion particulate complexed to montmorillonite clay.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/519cd09084264ef8ab5eac16275fdefe
work_keys_str_mv	AT tracyanichols intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT terryrspraker intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT taradrigg intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT crystalmeyerettreid intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT clarehoover intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT bradymichel intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT jifengbian intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT edwardhoover intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT thomasgidlewski intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT arubalachandran intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT katherineorourke intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT glennctelling intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT richardbowen intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT markdzabel intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay AT kurtcvercauteren intranasalinoculationofwhitetaileddeerodocoileusvirginianuswithlyophilizedchronicwastingdiseaseprionparticulatecomplexedtomontmorilloniteclay
_version_	1718422970201800704

Intranasal inoculation of white-tailed deer (Odocoileus virginianus) with lyophilized chronic wasting disease prion particulate complexed to montmorillonite clay.

Ejemplares similares