Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters

ABSTRACT: Sperm motility is a crucial trait in chicken production, and the epididymis is an essential organ in the reproductive system. Currently, the molecular mechanisms underlying sperm motility in the epididymis are unclear. In this study, 8 cDNA libraries and eight miRNA libraries were construc...

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Autores principales:	Kai Xing, Yu Chen, Liang Wang, Xueze Lv, Zheng Li, Xiaolong Qi, Xiangguo Wang, Longfei Xiao, Hemin Ni, Yong Guo, Xihui Sheng
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	transcriptome miRNA epididymis rooster sperm motility Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/519db255c5984d62be82dd45229cb9c7
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spelling	oai:doaj.org-article:519db255c5984d62be82dd45229cb9c72021-11-28T04:27:16ZEpididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters0032-579110.1016/j.psj.2021.101558https://doaj.org/article/519db255c5984d62be82dd45229cb9c72022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005800https://doaj.org/toc/0032-5791ABSTRACT: Sperm motility is a crucial trait in chicken production, and the epididymis is an essential organ in the reproductive system. Currently, the molecular mechanisms underlying sperm motility in the epididymis are unclear. In this study, 8 cDNA libraries and eight miRNA libraries were constructed from roosters (4 chickens per group) with diverse sperm motility. After a comparative analysis of epididymal transcriptomes, we detected 84 differentially expressed genes (DEGs) using the edgeR package. Integrated interpretation of DEGs indicated that MMP9, SLN, WT1, PLIN1, and LRRIQ1 are the most promising candidate genes affecting sperm motility in the epididymis of roosters. MiR-146a, mir-135b, and mir-205 could play important regulatory roles in sperm maturation, capacitation, and motility. Additionally, a comprehensive analysis of the mRNA and miRNAs transcriptomes in silico identified a promising gene-miRNA pair miR-135b-HPS5, which may be a vital regulator of sperm motility in the epididymis. Our findings provide novel integrated information of miRNAs and genes that shed light on the regulatory mechanisms of fertility in roosters.Kai XingYu ChenLiang WangXueze LvZheng LiXiaolong QiXiangguo WangLongfei XiaoHemin NiYong GuoXihui ShengElsevierarticletranscriptomemiRNAepididymisroostersperm motilityAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101558- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	transcriptome miRNA epididymis rooster sperm motility Animal culture SF1-1100
spellingShingle	transcriptome miRNA epididymis rooster sperm motility Animal culture SF1-1100 Kai Xing Yu Chen Liang Wang Xueze Lv Zheng Li Xiaolong Qi Xiangguo Wang Longfei Xiao Hemin Ni Yong Guo Xihui Sheng Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters
description	ABSTRACT: Sperm motility is a crucial trait in chicken production, and the epididymis is an essential organ in the reproductive system. Currently, the molecular mechanisms underlying sperm motility in the epididymis are unclear. In this study, 8 cDNA libraries and eight miRNA libraries were constructed from roosters (4 chickens per group) with diverse sperm motility. After a comparative analysis of epididymal transcriptomes, we detected 84 differentially expressed genes (DEGs) using the edgeR package. Integrated interpretation of DEGs indicated that MMP9, SLN, WT1, PLIN1, and LRRIQ1 are the most promising candidate genes affecting sperm motility in the epididymis of roosters. MiR-146a, mir-135b, and mir-205 could play important regulatory roles in sperm maturation, capacitation, and motility. Additionally, a comprehensive analysis of the mRNA and miRNAs transcriptomes in silico identified a promising gene-miRNA pair miR-135b-HPS5, which may be a vital regulator of sperm motility in the epididymis. Our findings provide novel integrated information of miRNAs and genes that shed light on the regulatory mechanisms of fertility in roosters.
format	article
author	Kai Xing Yu Chen Liang Wang Xueze Lv Zheng Li Xiaolong Qi Xiangguo Wang Longfei Xiao Hemin Ni Yong Guo Xihui Sheng
author_facet	Kai Xing Yu Chen Liang Wang Xueze Lv Zheng Li Xiaolong Qi Xiangguo Wang Longfei Xiao Hemin Ni Yong Guo Xihui Sheng
author_sort	Kai Xing
title	Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters
title_short	Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters
title_full	Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters
title_fullStr	Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters
title_full_unstemmed	Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters
title_sort	epididymal mrna and mirna transcriptome analyses reveal important genes and mirnas related to sperm motility in roosters
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/519db255c5984d62be82dd45229cb9c7
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Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters

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