Transcriptomic analyses reveal biosynthetic genes related to rosmarinic acid in Dracocephalum tanguticum

Abstract Dracocephalum tanguticum Maxim, a Lamiaceae species endemic to the Qinghai-Tibetan Plateau and adjacent regions, is an important ornamental, medicinal and aromatic herb. In this study, a comprehensive transcriptome of 18 libraries from six organs namely, roots, stems, leaves, sepals, flower...

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Autores principales:	Huie Li, Yaru Fu, Hao Sun, Yanfu Zhang, Xiaozhong Lan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/51a56f3c761f40609b697e446ee84267
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spelling	oai:doaj.org-article:51a56f3c761f40609b697e446ee842672021-12-02T11:41:21ZTranscriptomic analyses reveal biosynthetic genes related to rosmarinic acid in Dracocephalum tanguticum10.1038/s41598-017-00078-y2045-2322https://doaj.org/article/51a56f3c761f40609b697e446ee842672017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00078-yhttps://doaj.org/toc/2045-2322Abstract Dracocephalum tanguticum Maxim, a Lamiaceae species endemic to the Qinghai-Tibetan Plateau and adjacent regions, is an important ornamental, medicinal and aromatic herb. In this study, a comprehensive transcriptome of 18 libraries from six organs namely, roots, stems, leaves, sepals, flowers and seeds of D. tanguticum were generated. More than 100 Gb of sequence data were obtained and assembled de novo into 187,447 transcripts, including 151,463 unigenes, among which the six organs shared 17.7% (26,841). In addition, all unigenes were assigned to 362 pathways, in which ‘biosynthesis of secondary metabolites’ is the second enriched pathway. Furthermore, rosmarinic acid (RA) is one of the multifunctional phenolic bioactive compounds produced in some Lamiaceae species. The six organs of D. tanguticum were confirmed to produce RA. A total of 22 predicted biosynthetic genes related to RA from the transcriptome were further isolated. Two of these genes were identified as candidates by evaluating the correlation coefficient between the RA contents and the expression of the predicted biosynthetic genes in the six organs. The new sequence information will improve the knowledge of D. tanguticum, as well as provide a reference tool for future studies of biosynthetic genes related to RA in this species.Huie LiYaru FuHao SunYanfu ZhangXiaozhong LanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Huie Li Yaru Fu Hao Sun Yanfu Zhang Xiaozhong Lan Transcriptomic analyses reveal biosynthetic genes related to rosmarinic acid in Dracocephalum tanguticum
description	Abstract Dracocephalum tanguticum Maxim, a Lamiaceae species endemic to the Qinghai-Tibetan Plateau and adjacent regions, is an important ornamental, medicinal and aromatic herb. In this study, a comprehensive transcriptome of 18 libraries from six organs namely, roots, stems, leaves, sepals, flowers and seeds of D. tanguticum were generated. More than 100 Gb of sequence data were obtained and assembled de novo into 187,447 transcripts, including 151,463 unigenes, among which the six organs shared 17.7% (26,841). In addition, all unigenes were assigned to 362 pathways, in which ‘biosynthesis of secondary metabolites’ is the second enriched pathway. Furthermore, rosmarinic acid (RA) is one of the multifunctional phenolic bioactive compounds produced in some Lamiaceae species. The six organs of D. tanguticum were confirmed to produce RA. A total of 22 predicted biosynthetic genes related to RA from the transcriptome were further isolated. Two of these genes were identified as candidates by evaluating the correlation coefficient between the RA contents and the expression of the predicted biosynthetic genes in the six organs. The new sequence information will improve the knowledge of D. tanguticum, as well as provide a reference tool for future studies of biosynthetic genes related to RA in this species.
format	article
author	Huie Li Yaru Fu Hao Sun Yanfu Zhang Xiaozhong Lan
author_facet	Huie Li Yaru Fu Hao Sun Yanfu Zhang Xiaozhong Lan
author_sort	Huie Li
title	Transcriptomic analyses reveal biosynthetic genes related to rosmarinic acid in Dracocephalum tanguticum
title_short	Transcriptomic analyses reveal biosynthetic genes related to rosmarinic acid in Dracocephalum tanguticum
title_full	Transcriptomic analyses reveal biosynthetic genes related to rosmarinic acid in Dracocephalum tanguticum
title_fullStr	Transcriptomic analyses reveal biosynthetic genes related to rosmarinic acid in Dracocephalum tanguticum
title_full_unstemmed	Transcriptomic analyses reveal biosynthetic genes related to rosmarinic acid in Dracocephalum tanguticum
title_sort	transcriptomic analyses reveal biosynthetic genes related to rosmarinic acid in dracocephalum tanguticum
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/51a56f3c761f40609b697e446ee84267
work_keys_str_mv	AT huieli transcriptomicanalysesrevealbiosyntheticgenesrelatedtorosmarinicacidindracocephalumtanguticum AT yarufu transcriptomicanalysesrevealbiosyntheticgenesrelatedtorosmarinicacidindracocephalumtanguticum AT haosun transcriptomicanalysesrevealbiosyntheticgenesrelatedtorosmarinicacidindracocephalumtanguticum AT yanfuzhang transcriptomicanalysesrevealbiosyntheticgenesrelatedtorosmarinicacidindracocephalumtanguticum AT xiaozhonglan transcriptomicanalysesrevealbiosyntheticgenesrelatedtorosmarinicacidindracocephalumtanguticum
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Transcriptomic analyses reveal biosynthetic genes related to rosmarinic acid in Dracocephalum tanguticum

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