Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms

This laboratory module familiarizes students with flow cytometry while acquiring quantitative reasoning skills during data analysis. Leukocytes, also known as coelomocytes (including hyaline and granular amoebocytes, and chloragocytes), from Eisenia hortensis (earthworms) are isolated from the coelo...

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Main Author:	Sheryl L. Fuller-Espie
Format:	article
Language:	EN
Published:	American Society for Microbiology 2010
Subjects:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Online Access:	https://doaj.org/article/51a7be8ea3cf4d238f4de659eb4d7376
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spelling	oai:doaj.org-article:51a7be8ea3cf4d238f4de659eb4d73762021-11-15T15:04:12ZUsing Flow Cytometry to Measure Phagocytic Uptake in Earthworms10.1128/jmbe.v11i2.1351935-78851935-7877https://doaj.org/article/51a7be8ea3cf4d238f4de659eb4d73762010-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v11i2.135https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885This laboratory module familiarizes students with flow cytometry while acquiring quantitative reasoning skills during data analysis. Leukocytes, also known as coelomocytes (including hyaline and granular amoebocytes, and chloragocytes), from Eisenia hortensis (earthworms) are isolated from the coelomic cavity and used for phagocytosis of fluorescent Escherichia coli. Students learn how to set up in vitro cellular assays and become familiar with theoretical principles of flow cytometry. Histograms based on fluorescence and scatter properties combined with gating options permit students to restrict their analyses to particular subsets of coelomocytes when measuring phagocytosis, a fundamentally important innate immune mechanism used in earthworms. Statistical analysis of data is included in laboratory reports which serve as the primary assessment instrument.Sheryl L. Fuller-EspieAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 11, Iss 2, Pp 144-151 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Sheryl L. Fuller-Espie Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
description	This laboratory module familiarizes students with flow cytometry while acquiring quantitative reasoning skills during data analysis. Leukocytes, also known as coelomocytes (including hyaline and granular amoebocytes, and chloragocytes), from Eisenia hortensis (earthworms) are isolated from the coelomic cavity and used for phagocytosis of fluorescent Escherichia coli. Students learn how to set up in vitro cellular assays and become familiar with theoretical principles of flow cytometry. Histograms based on fluorescence and scatter properties combined with gating options permit students to restrict their analyses to particular subsets of coelomocytes when measuring phagocytosis, a fundamentally important innate immune mechanism used in earthworms. Statistical analysis of data is included in laboratory reports which serve as the primary assessment instrument.
format	article
author	Sheryl L. Fuller-Espie
author_facet	Sheryl L. Fuller-Espie
author_sort	Sheryl L. Fuller-Espie
title	Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
title_short	Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
title_full	Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
title_fullStr	Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
title_full_unstemmed	Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
title_sort	using flow cytometry to measure phagocytic uptake in earthworms
publisher	American Society for Microbiology
publishDate	2010
url	https://doaj.org/article/51a7be8ea3cf4d238f4de659eb4d7376
work_keys_str_mv	AT sheryllfullerespie usingflowcytometrytomeasurephagocyticuptakeinearthworms
_version_	1718428250049347584

Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms

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