Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms

Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms

This laboratory module familiarizes students with flow cytometry while acquiring quantitative reasoning skills during data analysis. Leukocytes, also known as coelomocytes (including hyaline and granular amoebocytes, and chloragocytes), from Eisenia hortensis (earthworms) are isolated from the coelo...

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Autor principal: Sheryl L. Fuller-Espie
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2010
Materias:
Special aspects of education
LC8-6691
Biology (General)
QH301-705.5
Acceso en línea:https://doaj.org/article/51a7be8ea3cf4d238f4de659eb4d7376
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spelling oai:doaj.org-article:51a7be8ea3cf4d238f4de659eb4d73762021-11-15T15:04:12ZUsing Flow Cytometry to Measure Phagocytic Uptake in Earthworms10.1128/jmbe.v11i2.1351935-78851935-7877https://doaj.org/article/51a7be8ea3cf4d238f4de659eb4d73762010-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v11i2.135https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885This laboratory module familiarizes students with flow cytometry while acquiring quantitative reasoning skills during data analysis. Leukocytes, also known as coelomocytes (including hyaline and granular amoebocytes, and chloragocytes), from Eisenia hortensis (earthworms) are isolated from the coelomic cavity and used for phagocytosis of fluorescent Escherichia coli. Students learn how to set up in vitro cellular assays and become familiar with theoretical principles of flow cytometry. Histograms based on fluorescence and scatter properties combined with gating options permit students to restrict their analyses to particular subsets of coelomocytes when measuring phagocytosis, a fundamentally important innate immune mechanism used in earthworms. Statistical analysis of data is included in laboratory reports which serve as the primary assessment instrument.Sheryl L. Fuller-EspieAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 11, Iss 2, Pp 144-151 (2010)
institution DOAJ
collection DOAJ
language EN
topic Special aspects of education
LC8-6691
Biology (General)
QH301-705.5
spellingShingle Special aspects of education
LC8-6691
Biology (General)
QH301-705.5
Sheryl L. Fuller-Espie
Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
description This laboratory module familiarizes students with flow cytometry while acquiring quantitative reasoning skills during data analysis. Leukocytes, also known as coelomocytes (including hyaline and granular amoebocytes, and chloragocytes), from Eisenia hortensis (earthworms) are isolated from the coelomic cavity and used for phagocytosis of fluorescent Escherichia coli. Students learn how to set up in vitro cellular assays and become familiar with theoretical principles of flow cytometry. Histograms based on fluorescence and scatter properties combined with gating options permit students to restrict their analyses to particular subsets of coelomocytes when measuring phagocytosis, a fundamentally important innate immune mechanism used in earthworms. Statistical analysis of data is included in laboratory reports which serve as the primary assessment instrument.
format article
author Sheryl L. Fuller-Espie
author_facet Sheryl L. Fuller-Espie
author_sort Sheryl L. Fuller-Espie
title Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
title_short Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
title_full Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
title_fullStr Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
title_full_unstemmed Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
title_sort using flow cytometry to measure phagocytic uptake in earthworms
publisher American Society for Microbiology
publishDate 2010
url https://doaj.org/article/51a7be8ea3cf4d238f4de659eb4d7376
work_keys_str_mv AT sheryllfullerespie usingflowcytometrytomeasurephagocyticuptakeinearthworms
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