Demonstration of Interposed Modular Hydrogel Sheet for Multicellular Analysis in a Microfluidic Assembly Platform

Abstract Hydrogel sheets have emerged as a promising biomaterial scaffold for the encapsulation and transfer of multicellular structures. Although the improvement of the chemical interactions and the design of micro-scaled geometry have contributed to the development of multipurpose hydrogel scaffol...

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Autores principales:	Chae Yun Bae, Jaejung Son, Hail Kim, Je-Kyun Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/51a7f846cb8b4d90b8afc0e6865dfc21
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spelling	oai:doaj.org-article:51a7f846cb8b4d90b8afc0e6865dfc212021-12-02T12:30:37ZDemonstration of Interposed Modular Hydrogel Sheet for Multicellular Analysis in a Microfluidic Assembly Platform10.1038/s41598-017-01363-62045-2322https://doaj.org/article/51a7f846cb8b4d90b8afc0e6865dfc212017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01363-6https://doaj.org/toc/2045-2322Abstract Hydrogel sheets have emerged as a promising biomaterial scaffold for the encapsulation and transfer of multicellular structures. Although the improvement of the chemical interactions and the design of micro-scaled geometry have contributed to the development of multipurpose hydrogel scaffolds, the application of hydrogel sheets to assess multicellular structures is still challenging. To expand the technical applicability of hydrogel sheets, we here demonstrate that a single layer of the hydrogel sheet can be integrated as an interposed module in a microfluidic device for multicellular analysis. As a cell culture unit, encapsulated pancreatic insulinoma (MIN6) cells in the hydrogel sheet were labeled and examined via multiple microchannels. After obtaining simultaneously multi-labeled cells in the hydrogel sheet that had been incorporated into the microfluidic device, each modular hydrogel sheet was also recoverable and re-cultured without any distortion. The modular hydrogel sheet can be simply manipulated and conserved as a multicellular module in a three-dimensional (3D) in vitro culture platform. Using the modular concept of hydrogel sheets capable of cell culture and/or assay, an integrated multicellular analysis in the microfluidic device is expected to improve accessibility, scalability, and practicality for end users.Chae Yun BaeJaejung SonHail KimJe-Kyun ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chae Yun Bae Jaejung Son Hail Kim Je-Kyun Park Demonstration of Interposed Modular Hydrogel Sheet for Multicellular Analysis in a Microfluidic Assembly Platform
description	Abstract Hydrogel sheets have emerged as a promising biomaterial scaffold for the encapsulation and transfer of multicellular structures. Although the improvement of the chemical interactions and the design of micro-scaled geometry have contributed to the development of multipurpose hydrogel scaffolds, the application of hydrogel sheets to assess multicellular structures is still challenging. To expand the technical applicability of hydrogel sheets, we here demonstrate that a single layer of the hydrogel sheet can be integrated as an interposed module in a microfluidic device for multicellular analysis. As a cell culture unit, encapsulated pancreatic insulinoma (MIN6) cells in the hydrogel sheet were labeled and examined via multiple microchannels. After obtaining simultaneously multi-labeled cells in the hydrogel sheet that had been incorporated into the microfluidic device, each modular hydrogel sheet was also recoverable and re-cultured without any distortion. The modular hydrogel sheet can be simply manipulated and conserved as a multicellular module in a three-dimensional (3D) in vitro culture platform. Using the modular concept of hydrogel sheets capable of cell culture and/or assay, an integrated multicellular analysis in the microfluidic device is expected to improve accessibility, scalability, and practicality for end users.
format	article
author	Chae Yun Bae Jaejung Son Hail Kim Je-Kyun Park
author_facet	Chae Yun Bae Jaejung Son Hail Kim Je-Kyun Park
author_sort	Chae Yun Bae
title	Demonstration of Interposed Modular Hydrogel Sheet for Multicellular Analysis in a Microfluidic Assembly Platform
title_short	Demonstration of Interposed Modular Hydrogel Sheet for Multicellular Analysis in a Microfluidic Assembly Platform
title_full	Demonstration of Interposed Modular Hydrogel Sheet for Multicellular Analysis in a Microfluidic Assembly Platform
title_fullStr	Demonstration of Interposed Modular Hydrogel Sheet for Multicellular Analysis in a Microfluidic Assembly Platform
title_full_unstemmed	Demonstration of Interposed Modular Hydrogel Sheet for Multicellular Analysis in a Microfluidic Assembly Platform
title_sort	demonstration of interposed modular hydrogel sheet for multicellular analysis in a microfluidic assembly platform
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/51a7f846cb8b4d90b8afc0e6865dfc21
work_keys_str_mv	AT chaeyunbae demonstrationofinterposedmodularhydrogelsheetformulticellularanalysisinamicrofluidicassemblyplatform AT jaejungson demonstrationofinterposedmodularhydrogelsheetformulticellularanalysisinamicrofluidicassemblyplatform AT hailkim demonstrationofinterposedmodularhydrogelsheetformulticellularanalysisinamicrofluidicassemblyplatform AT jekyunpark demonstrationofinterposedmodularhydrogelsheetformulticellularanalysisinamicrofluidicassemblyplatform
_version_	1718394335254282240

Demonstration of Interposed Modular Hydrogel Sheet for Multicellular Analysis in a Microfluidic Assembly Platform

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