Rhizobium Symbiotic Capacity Shapes Root-Associated Microbiomes in Soybean
Root-microbiome interactions are of central importance for plant performance and yield. A distinctive feature of legumes is that they engage in symbiosis with N2-fixing rhizobia. If and how the rhizobial symbiotic capacity modulates root-associated microbiomes are still not yet well understood. We d...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/51ab66b7548640e1bdc7c61d18a01d0b |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:51ab66b7548640e1bdc7c61d18a01d0b |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:51ab66b7548640e1bdc7c61d18a01d0b2021-12-02T11:41:52ZRhizobium Symbiotic Capacity Shapes Root-Associated Microbiomes in Soybean1664-302X10.3389/fmicb.2021.709012https://doaj.org/article/51ab66b7548640e1bdc7c61d18a01d0b2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.709012/fullhttps://doaj.org/toc/1664-302XRoot-microbiome interactions are of central importance for plant performance and yield. A distinctive feature of legumes is that they engage in symbiosis with N2-fixing rhizobia. If and how the rhizobial symbiotic capacity modulates root-associated microbiomes are still not yet well understood. We determined root-associated microbiomes of soybean inoculated with wild type (WT) or a noeI mutant of Bradyrhizobium diazoefficiens USDA 110 by amplicon sequencing. UPLC-MS/MS was used to analyze root exudates. The noeI gene is responsible for fucose-methylation of Nod factor secreted by USDA 110 WT strain. Soybean roots inoculated with the noeI mutant showed a significant decrease in nodulation and root-flavonoid exudation compared to roots inoculated with WT strain. The noeI mutant-inoculated roots exhibited strong changes in microbiome assembly in the rhizosphere and rhizoplane, including reduced diversity, changed co-occurrence interactions and a substantial depletion of root microbes. Root exudates and soil physiochemical properties were significantly correlated with microbial community shift in the rhizosphere between different rhizobial treatments. These results illustrate that rhizobial symbiotic capacity dramatically alters root-associated microbiomes, in which root exudation and edaphic patterns play a vital role. This study has important implications for understanding the evolution of plant-microbiome interactions.Yuanhui LiuYuanhui LiuYuanhui LiuBin MaBin MaBin MaWenfeng ChenKlaus SchlaeppiKlaus SchlaeppiMatthias ErbErinne StirlingErinne StirlingErinne StirlingErinne StirlingLingfei HuLingfei HuEntao WangYunzeng ZhangKankan ZhaoKankan ZhaoZhijiang LuZhijiang LuShudi YeShudi YeJianming XuJianming XuFrontiers Media S.A.articleroot-associated microbiomesymbiosisrhizobiaGlycine maxroot exudationMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
root-associated microbiome symbiosis rhizobia Glycine max root exudation Microbiology QR1-502 |
| spellingShingle |
root-associated microbiome symbiosis rhizobia Glycine max root exudation Microbiology QR1-502 Yuanhui Liu Yuanhui Liu Yuanhui Liu Bin Ma Bin Ma Bin Ma Wenfeng Chen Klaus Schlaeppi Klaus Schlaeppi Matthias Erb Erinne Stirling Erinne Stirling Erinne Stirling Erinne Stirling Lingfei Hu Lingfei Hu Entao Wang Yunzeng Zhang Kankan Zhao Kankan Zhao Zhijiang Lu Zhijiang Lu Shudi Ye Shudi Ye Jianming Xu Jianming Xu Rhizobium Symbiotic Capacity Shapes Root-Associated Microbiomes in Soybean |
| description |
Root-microbiome interactions are of central importance for plant performance and yield. A distinctive feature of legumes is that they engage in symbiosis with N2-fixing rhizobia. If and how the rhizobial symbiotic capacity modulates root-associated microbiomes are still not yet well understood. We determined root-associated microbiomes of soybean inoculated with wild type (WT) or a noeI mutant of Bradyrhizobium diazoefficiens USDA 110 by amplicon sequencing. UPLC-MS/MS was used to analyze root exudates. The noeI gene is responsible for fucose-methylation of Nod factor secreted by USDA 110 WT strain. Soybean roots inoculated with the noeI mutant showed a significant decrease in nodulation and root-flavonoid exudation compared to roots inoculated with WT strain. The noeI mutant-inoculated roots exhibited strong changes in microbiome assembly in the rhizosphere and rhizoplane, including reduced diversity, changed co-occurrence interactions and a substantial depletion of root microbes. Root exudates and soil physiochemical properties were significantly correlated with microbial community shift in the rhizosphere between different rhizobial treatments. These results illustrate that rhizobial symbiotic capacity dramatically alters root-associated microbiomes, in which root exudation and edaphic patterns play a vital role. This study has important implications for understanding the evolution of plant-microbiome interactions. |
| format |
article |
| author |
Yuanhui Liu Yuanhui Liu Yuanhui Liu Bin Ma Bin Ma Bin Ma Wenfeng Chen Klaus Schlaeppi Klaus Schlaeppi Matthias Erb Erinne Stirling Erinne Stirling Erinne Stirling Erinne Stirling Lingfei Hu Lingfei Hu Entao Wang Yunzeng Zhang Kankan Zhao Kankan Zhao Zhijiang Lu Zhijiang Lu Shudi Ye Shudi Ye Jianming Xu Jianming Xu |
| author_facet |
Yuanhui Liu Yuanhui Liu Yuanhui Liu Bin Ma Bin Ma Bin Ma Wenfeng Chen Klaus Schlaeppi Klaus Schlaeppi Matthias Erb Erinne Stirling Erinne Stirling Erinne Stirling Erinne Stirling Lingfei Hu Lingfei Hu Entao Wang Yunzeng Zhang Kankan Zhao Kankan Zhao Zhijiang Lu Zhijiang Lu Shudi Ye Shudi Ye Jianming Xu Jianming Xu |
| author_sort |
Yuanhui Liu |
| title |
Rhizobium Symbiotic Capacity Shapes Root-Associated Microbiomes in Soybean |
| title_short |
Rhizobium Symbiotic Capacity Shapes Root-Associated Microbiomes in Soybean |
| title_full |
Rhizobium Symbiotic Capacity Shapes Root-Associated Microbiomes in Soybean |
| title_fullStr |
Rhizobium Symbiotic Capacity Shapes Root-Associated Microbiomes in Soybean |
| title_full_unstemmed |
Rhizobium Symbiotic Capacity Shapes Root-Associated Microbiomes in Soybean |
| title_sort |
rhizobium symbiotic capacity shapes root-associated microbiomes in soybean |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/51ab66b7548640e1bdc7c61d18a01d0b |
| work_keys_str_mv |
AT yuanhuiliu rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT yuanhuiliu rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT yuanhuiliu rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT binma rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT binma rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT binma rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT wenfengchen rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT klausschlaeppi rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT klausschlaeppi rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT matthiaserb rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT erinnestirling rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT erinnestirling rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT erinnestirling rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT erinnestirling rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT lingfeihu rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT lingfeihu rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT entaowang rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT yunzengzhang rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT kankanzhao rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT kankanzhao rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT zhijianglu rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT zhijianglu rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT shudiye rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT shudiye rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT jianmingxu rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean AT jianmingxu rhizobiumsymbioticcapacityshapesrootassociatedmicrobiomesinsoybean |
| _version_ |
1718395397919997952 |