Fabrication Of Gold Nanoparticles In Absence Of Surfactant As In Vitro Carrier Of Plasmid DNA

Rania S Abdel-Rashid,1 Samia M Omar,1,2 Mohammed S Teiama,1 Ahmed Khairy,3 Mohamed Magdy,4 Badawi Anis5 1Department of Pharmaceutics, Faculty of Pharmacy, Helwan University, Cairo, Egypt; 2Department of Pharmaceutics, Faculty of Pharmacy, Ahram Canadian University, Giza, Egypt; 3Department of Pharma...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Abdel-Rashid RS, Omar SM, Teiama MS, Khairy A, Magdy M, Anis B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Gold Nanoparticles Plasmid DNA Human Lung Cancer Transfection Gene delivery. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/51b041e823cb41dda32193f57e8b9487
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:51b041e823cb41dda32193f57e8b9487
record_format	dspace
spelling	oai:doaj.org-article:51b041e823cb41dda32193f57e8b94872021-12-02T04:45:09ZFabrication Of Gold Nanoparticles In Absence Of Surfactant As In Vitro Carrier Of Plasmid DNA1178-2013https://doaj.org/article/51b041e823cb41dda32193f57e8b94872019-10-01T00:00:00Zhttps://www.dovepress.com/fabrication-of-gold-nanoparticles-in-absence-of-surfactant-as-in-vitro-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Rania S Abdel-Rashid,1 Samia M Omar,1,2 Mohammed S Teiama,1 Ahmed Khairy,3 Mohamed Magdy,4 Badawi Anis5 1Department of Pharmaceutics, Faculty of Pharmacy, Helwan University, Cairo, Egypt; 2Department of Pharmaceutics, Faculty of Pharmacy, Ahram Canadian University, Giza, Egypt; 3Department of Pharmaceutics, National Organization for Drug Control and Research (NODCAR), Cairo, Egypt; 4Ministry of Interior, Administration of Criminal Evidence, Cairo, Egypt; 5Spectroscopy Department, Physics Division, National Research Centre, Giza, EgyptCorrespondence: Rania S Abdel-RashidDepartment of Pharmaceutics, Faculty of Pharmacy, Helwan University, Ain Helwan, Cairo 11795, EgyptTel +201156995596Fax +2025553487Email sansoova@yahoo.comPurpose: This work aimed to synthesize surfactant-free AuNPs for targeted delivery of plasmid DNA encoded p53 gene and to avoid conventional production method of Gold nanoparticles (AuNPs) which may adversely affect the final shape, diversity, and size due to accumulation of the formulated surfactant – gold complex to the surface.Methods: The AuNPs were fabricated using seeded-growth method with L-Cystine methyl ester hydrochloride as capping agent, then loaded with plasmid DNA encoded p53 gene. The resultant AuNPs and AuNPs-p53 complex were evaluated for physical characteristics and morphology. Confirmation of complex formation was performed using gel electrophoresis. Furthermore, the efficient delivery and cytotoxicity behavior of the encoded gene were examined on both healthy lung cells (WI38) and cancerous lung cells (A549).Results: L-cysteine methyl ester hydrochloride AuNPs showed acceptable physical characteristics (30 nm, +36.9 mv, and spherical morphology). P53 attachment to AuNPs was confirmed by gel electrophoresis. The RT-PCR proved the overexpression of p53 by the fabricated AuNPs-p53 complex. The high percentage of cell viability in normal lung cell line (WI 38) proved the safety of L-cysteine methyl ester functionalized AuNPs. Additionally, the apoptotic effect due to expression of p53 gene loaded on AuNPs was only prominent in lung cancer cell line (A549), revealing selectivity and targeting efficiency of anticancer AuNPs-p53 complex.Conclusion: AuNPs can be considered as a potential delivery system for effective transfection of plasmid DNA which can be used for successful treatment of cancer.Keywords: gold nanoparticles, plasmid DNA, human lung cancer, transfection, gene deliveryAbdel-Rashid RSOmar SMTeiama MSKhairy AMagdy MAnis BDove Medical PressarticleGold NanoparticlesPlasmid DNAHuman Lung CancerTransfectionGene delivery.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 8399-8408 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gold Nanoparticles Plasmid DNA Human Lung Cancer Transfection Gene delivery. Medicine (General) R5-920
spellingShingle	Gold Nanoparticles Plasmid DNA Human Lung Cancer Transfection Gene delivery. Medicine (General) R5-920 Abdel-Rashid RS Omar SM Teiama MS Khairy A Magdy M Anis B Fabrication Of Gold Nanoparticles In Absence Of Surfactant As In Vitro Carrier Of Plasmid DNA
description	Rania S Abdel-Rashid,1 Samia M Omar,1,2 Mohammed S Teiama,1 Ahmed Khairy,3 Mohamed Magdy,4 Badawi Anis5 1Department of Pharmaceutics, Faculty of Pharmacy, Helwan University, Cairo, Egypt; 2Department of Pharmaceutics, Faculty of Pharmacy, Ahram Canadian University, Giza, Egypt; 3Department of Pharmaceutics, National Organization for Drug Control and Research (NODCAR), Cairo, Egypt; 4Ministry of Interior, Administration of Criminal Evidence, Cairo, Egypt; 5Spectroscopy Department, Physics Division, National Research Centre, Giza, EgyptCorrespondence: Rania S Abdel-RashidDepartment of Pharmaceutics, Faculty of Pharmacy, Helwan University, Ain Helwan, Cairo 11795, EgyptTel +201156995596Fax +2025553487Email sansoova@yahoo.comPurpose: This work aimed to synthesize surfactant-free AuNPs for targeted delivery of plasmid DNA encoded p53 gene and to avoid conventional production method of Gold nanoparticles (AuNPs) which may adversely affect the final shape, diversity, and size due to accumulation of the formulated surfactant – gold complex to the surface.Methods: The AuNPs were fabricated using seeded-growth method with L-Cystine methyl ester hydrochloride as capping agent, then loaded with plasmid DNA encoded p53 gene. The resultant AuNPs and AuNPs-p53 complex were evaluated for physical characteristics and morphology. Confirmation of complex formation was performed using gel electrophoresis. Furthermore, the efficient delivery and cytotoxicity behavior of the encoded gene were examined on both healthy lung cells (WI38) and cancerous lung cells (A549).Results: L-cysteine methyl ester hydrochloride AuNPs showed acceptable physical characteristics (30 nm, +36.9 mv, and spherical morphology). P53 attachment to AuNPs was confirmed by gel electrophoresis. The RT-PCR proved the overexpression of p53 by the fabricated AuNPs-p53 complex. The high percentage of cell viability in normal lung cell line (WI 38) proved the safety of L-cysteine methyl ester functionalized AuNPs. Additionally, the apoptotic effect due to expression of p53 gene loaded on AuNPs was only prominent in lung cancer cell line (A549), revealing selectivity and targeting efficiency of anticancer AuNPs-p53 complex.Conclusion: AuNPs can be considered as a potential delivery system for effective transfection of plasmid DNA which can be used for successful treatment of cancer.Keywords: gold nanoparticles, plasmid DNA, human lung cancer, transfection, gene delivery
format	article
author	Abdel-Rashid RS Omar SM Teiama MS Khairy A Magdy M Anis B
author_facet	Abdel-Rashid RS Omar SM Teiama MS Khairy A Magdy M Anis B
author_sort	Abdel-Rashid RS
title	Fabrication Of Gold Nanoparticles In Absence Of Surfactant As In Vitro Carrier Of Plasmid DNA
title_short	Fabrication Of Gold Nanoparticles In Absence Of Surfactant As In Vitro Carrier Of Plasmid DNA
title_full	Fabrication Of Gold Nanoparticles In Absence Of Surfactant As In Vitro Carrier Of Plasmid DNA
title_fullStr	Fabrication Of Gold Nanoparticles In Absence Of Surfactant As In Vitro Carrier Of Plasmid DNA
title_full_unstemmed	Fabrication Of Gold Nanoparticles In Absence Of Surfactant As In Vitro Carrier Of Plasmid DNA
title_sort	fabrication of gold nanoparticles in absence of surfactant as in vitro carrier of plasmid dna
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/51b041e823cb41dda32193f57e8b9487
work_keys_str_mv	AT abdelrashidrs fabricationofgoldnanoparticlesinabsenceofsurfactantasinvitrocarrierofplasmiddna AT omarsm fabricationofgoldnanoparticlesinabsenceofsurfactantasinvitrocarrierofplasmiddna AT teiamams fabricationofgoldnanoparticlesinabsenceofsurfactantasinvitrocarrierofplasmiddna AT khairya fabricationofgoldnanoparticlesinabsenceofsurfactantasinvitrocarrierofplasmiddna AT magdym fabricationofgoldnanoparticlesinabsenceofsurfactantasinvitrocarrierofplasmiddna AT anisb fabricationofgoldnanoparticlesinabsenceofsurfactantasinvitrocarrierofplasmiddna
_version_	1718401082874396672

Fabrication Of Gold Nanoparticles In Absence Of Surfactant As In Vitro Carrier Of Plasmid DNA

Ejemplares similares