Preclinical testing of chimeric antigen receptor T cells in neuroblastoma mouse models

Summary: The translation of chimeric antigen receptor (CAR) T cell therapy for pediatric solid tumors is limited by the lack of preclinical models that fully recapitulate solid tumor biology. We describe steps to implement neuroblastoma metastatic and orthotopic mouse models. We delineate an analysi...

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Autores principales:	Nan Li, Rosa Nguyen, Carol J. Thiele, Mitchell Ho
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell culture Cell-based Assays Cancer Immunology Model Organisms Molecular Biology Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/51b45698cd264ae3a0c1ea891efc33b3
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spelling	oai:doaj.org-article:51b45698cd264ae3a0c1ea891efc33b32021-11-12T04:47:42ZPreclinical testing of chimeric antigen receptor T cells in neuroblastoma mouse models2666-166710.1016/j.xpro.2021.100942https://doaj.org/article/51b45698cd264ae3a0c1ea891efc33b32021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006481https://doaj.org/toc/2666-1667Summary: The translation of chimeric antigen receptor (CAR) T cell therapy for pediatric solid tumors is limited by the lack of preclinical models that fully recapitulate solid tumor biology. We describe steps to implement neuroblastoma metastatic and orthotopic mouse models. We delineate an analysis pipeline to quantify the efficacy and determine the immunological characteristics of both CAR T and tumor cells in these models. Both mouse models can be applied to evaluate other experimental therapies for neuroblastoma.For complete details on the use and execution of this protocol, please refer to Li et al. (2021).Nan LiRosa NguyenCarol J. ThieleMitchell HoElsevierarticleCell cultureCell-based AssaysCancerImmunologyModel OrganismsMolecular BiologyScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100942- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell culture Cell-based Assays Cancer Immunology Model Organisms Molecular Biology Science (General) Q1-390
spellingShingle	Cell culture Cell-based Assays Cancer Immunology Model Organisms Molecular Biology Science (General) Q1-390 Nan Li Rosa Nguyen Carol J. Thiele Mitchell Ho Preclinical testing of chimeric antigen receptor T cells in neuroblastoma mouse models
description	Summary: The translation of chimeric antigen receptor (CAR) T cell therapy for pediatric solid tumors is limited by the lack of preclinical models that fully recapitulate solid tumor biology. We describe steps to implement neuroblastoma metastatic and orthotopic mouse models. We delineate an analysis pipeline to quantify the efficacy and determine the immunological characteristics of both CAR T and tumor cells in these models. Both mouse models can be applied to evaluate other experimental therapies for neuroblastoma.For complete details on the use and execution of this protocol, please refer to Li et al. (2021).
format	article
author	Nan Li Rosa Nguyen Carol J. Thiele Mitchell Ho
author_facet	Nan Li Rosa Nguyen Carol J. Thiele Mitchell Ho
author_sort	Nan Li
title	Preclinical testing of chimeric antigen receptor T cells in neuroblastoma mouse models
title_short	Preclinical testing of chimeric antigen receptor T cells in neuroblastoma mouse models
title_full	Preclinical testing of chimeric antigen receptor T cells in neuroblastoma mouse models
title_fullStr	Preclinical testing of chimeric antigen receptor T cells in neuroblastoma mouse models
title_full_unstemmed	Preclinical testing of chimeric antigen receptor T cells in neuroblastoma mouse models
title_sort	preclinical testing of chimeric antigen receptor t cells in neuroblastoma mouse models
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/51b45698cd264ae3a0c1ea891efc33b3
work_keys_str_mv	AT nanli preclinicaltestingofchimericantigenreceptortcellsinneuroblastomamousemodels AT rosanguyen preclinicaltestingofchimericantigenreceptortcellsinneuroblastomamousemodels AT caroljthiele preclinicaltestingofchimericantigenreceptortcellsinneuroblastomamousemodels AT mitchellho preclinicaltestingofchimericantigenreceptortcellsinneuroblastomamousemodels
_version_	1718431229567565824

Preclinical testing of chimeric antigen receptor T cells in neuroblastoma mouse models

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