Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay

Our understanding of cytosolic delivery is hindered by existing methods for quantification which suffer from being indirect and showing low sensitivity. Here the authors report a SLEEQ (Split Luciferase Endosomal Escape Quantification) assay to assess cytosolic delivery of cell-penetrating peptides.

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Autores principales:	Serena L. Y. Teo, Joshua J. Rennick, Daniel Yuen, Hareth Al-Wassiti, Angus P. R. Johnston, Colin W. Pouton
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/51c2a8863f7b4866907c4bcbaa87460c
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spelling	oai:doaj.org-article:51c2a8863f7b4866907c4bcbaa87460c2021-12-02T17:23:59ZUnravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay10.1038/s41467-021-23997-x2041-1723https://doaj.org/article/51c2a8863f7b4866907c4bcbaa87460c2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23997-xhttps://doaj.org/toc/2041-1723Our understanding of cytosolic delivery is hindered by existing methods for quantification which suffer from being indirect and showing low sensitivity. Here the authors report a SLEEQ (Split Luciferase Endosomal Escape Quantification) assay to assess cytosolic delivery of cell-penetrating peptides.Serena L. Y. TeoJoshua J. RennickDaniel YuenHareth Al-WassitiAngus P. R. JohnstonColin W. PoutonNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Serena L. Y. Teo Joshua J. Rennick Daniel Yuen Hareth Al-Wassiti Angus P. R. Johnston Colin W. Pouton Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay
description	Our understanding of cytosolic delivery is hindered by existing methods for quantification which suffer from being indirect and showing low sensitivity. Here the authors report a SLEEQ (Split Luciferase Endosomal Escape Quantification) assay to assess cytosolic delivery of cell-penetrating peptides.
format	article
author	Serena L. Y. Teo Joshua J. Rennick Daniel Yuen Hareth Al-Wassiti Angus P. R. Johnston Colin W. Pouton
author_facet	Serena L. Y. Teo Joshua J. Rennick Daniel Yuen Hareth Al-Wassiti Angus P. R. Johnston Colin W. Pouton
author_sort	Serena L. Y. Teo
title	Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay
title_short	Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay
title_full	Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay
title_fullStr	Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay
title_full_unstemmed	Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay
title_sort	unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/51c2a8863f7b4866907c4bcbaa87460c
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Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay

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