Genome-wide association analysis permits characterization of Stagonospora nodorum blotch (SNB) resistance in hard winter wheat

Abstract Stagonospora nodorum blotch (SNB) is an economically important wheat disease caused by the necrotrophic fungus Parastagonospora nodorum. SNB resistance in wheat is controlled by several quantitative trait loci (QTLs). Thus, identifying novel resistance/susceptibility QTLs is crucial for con...

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Autores principales:	Rami AlTameemi, Harsimardeep S. Gill, Shaukat Ali, Girma Ayana, Jyotirmoy Halder, Jagdeep S. Sidhu, Upinder S. Gill, Brent Turnipseed, Jose L. Gonzalez Hernandez, Sunish K. Sehgal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/51dc33343a9243e8b48c64928c6fddb5
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spelling	oai:doaj.org-article:51dc33343a9243e8b48c64928c6fddb52021-12-02T17:41:32ZGenome-wide association analysis permits characterization of Stagonospora nodorum blotch (SNB) resistance in hard winter wheat10.1038/s41598-021-91515-62045-2322https://doaj.org/article/51dc33343a9243e8b48c64928c6fddb52021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91515-6https://doaj.org/toc/2045-2322Abstract Stagonospora nodorum blotch (SNB) is an economically important wheat disease caused by the necrotrophic fungus Parastagonospora nodorum. SNB resistance in wheat is controlled by several quantitative trait loci (QTLs). Thus, identifying novel resistance/susceptibility QTLs is crucial for continuous improvement of the SNB resistance. Here, the hard winter wheat association mapping panel (HWWAMP) comprising accessions from breeding programs in the Great Plains region of the US, was evaluated for SNB resistance and necrotrophic effectors (NEs) sensitivity at the seedling stage. A genome-wide association study (GWAS) was performed to identify single‐nucleotide polymorphism (SNP) markers associated with SNB resistance and effectors sensitivity. We found seven significant associations for SNB resistance/susceptibility distributed over chromosomes 1B, 2AL, 2DS, 4AL, 5BL, 6BS, and 7AL. Two new QTLs for SNB resistance/susceptibility at the seedling stage were identified on chromosomes 6BS and 7AL, whereas five QTLs previously reported in diverse germplasms were validated. Allele stacking analysis at seven QTLs explained the additive and complex nature of SNB resistance. We identified accessions (‘Pioneer-2180’ and ‘Shocker’) with favorable alleles at five of the seven identified loci, exhibiting a high level of resistance against SNB. Further, GWAS for sensitivity to NEs uncovered significant associations for SnToxA and SnTox3, co-locating with previously identified host sensitivity genes (Tsn1 and Snn3). Candidate region analysis for SNB resistance revealed 35 genes of putative interest with plant defense response-related functions. The QTLs identified and validated in this study could be easily employed in breeding programs using the associated markers to enhance the SNB resistance in hard winter wheat.Rami AlTameemiHarsimardeep S. GillShaukat AliGirma AyanaJyotirmoy HalderJagdeep S. SidhuUpinder S. GillBrent TurnipseedJose L. Gonzalez HernandezSunish K. SehgalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rami AlTameemi Harsimardeep S. Gill Shaukat Ali Girma Ayana Jyotirmoy Halder Jagdeep S. Sidhu Upinder S. Gill Brent Turnipseed Jose L. Gonzalez Hernandez Sunish K. Sehgal Genome-wide association analysis permits characterization of Stagonospora nodorum blotch (SNB) resistance in hard winter wheat
description	Abstract Stagonospora nodorum blotch (SNB) is an economically important wheat disease caused by the necrotrophic fungus Parastagonospora nodorum. SNB resistance in wheat is controlled by several quantitative trait loci (QTLs). Thus, identifying novel resistance/susceptibility QTLs is crucial for continuous improvement of the SNB resistance. Here, the hard winter wheat association mapping panel (HWWAMP) comprising accessions from breeding programs in the Great Plains region of the US, was evaluated for SNB resistance and necrotrophic effectors (NEs) sensitivity at the seedling stage. A genome-wide association study (GWAS) was performed to identify single‐nucleotide polymorphism (SNP) markers associated with SNB resistance and effectors sensitivity. We found seven significant associations for SNB resistance/susceptibility distributed over chromosomes 1B, 2AL, 2DS, 4AL, 5BL, 6BS, and 7AL. Two new QTLs for SNB resistance/susceptibility at the seedling stage were identified on chromosomes 6BS and 7AL, whereas five QTLs previously reported in diverse germplasms were validated. Allele stacking analysis at seven QTLs explained the additive and complex nature of SNB resistance. We identified accessions (‘Pioneer-2180’ and ‘Shocker’) with favorable alleles at five of the seven identified loci, exhibiting a high level of resistance against SNB. Further, GWAS for sensitivity to NEs uncovered significant associations for SnToxA and SnTox3, co-locating with previously identified host sensitivity genes (Tsn1 and Snn3). Candidate region analysis for SNB resistance revealed 35 genes of putative interest with plant defense response-related functions. The QTLs identified and validated in this study could be easily employed in breeding programs using the associated markers to enhance the SNB resistance in hard winter wheat.
format	article
author	Rami AlTameemi Harsimardeep S. Gill Shaukat Ali Girma Ayana Jyotirmoy Halder Jagdeep S. Sidhu Upinder S. Gill Brent Turnipseed Jose L. Gonzalez Hernandez Sunish K. Sehgal
author_facet	Rami AlTameemi Harsimardeep S. Gill Shaukat Ali Girma Ayana Jyotirmoy Halder Jagdeep S. Sidhu Upinder S. Gill Brent Turnipseed Jose L. Gonzalez Hernandez Sunish K. Sehgal
author_sort	Rami AlTameemi
title	Genome-wide association analysis permits characterization of Stagonospora nodorum blotch (SNB) resistance in hard winter wheat
title_short	Genome-wide association analysis permits characterization of Stagonospora nodorum blotch (SNB) resistance in hard winter wheat
title_full	Genome-wide association analysis permits characterization of Stagonospora nodorum blotch (SNB) resistance in hard winter wheat
title_fullStr	Genome-wide association analysis permits characterization of Stagonospora nodorum blotch (SNB) resistance in hard winter wheat
title_full_unstemmed	Genome-wide association analysis permits characterization of Stagonospora nodorum blotch (SNB) resistance in hard winter wheat
title_sort	genome-wide association analysis permits characterization of stagonospora nodorum blotch (snb) resistance in hard winter wheat
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/51dc33343a9243e8b48c64928c6fddb5
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Genome-wide association analysis permits characterization of Stagonospora nodorum blotch (SNB) resistance in hard winter wheat

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