Selenocystine against methyl mercury cytotoxicity in HepG2 cells

Abstract Methyl mercury (MeHg) is a highly toxic substance and the effect of selenium against MeHg toxicity is a hot topic. Until now, no related works have been reported from the view of the point of elemental speciation which is promising to study the mechanism at the molecular level. In this work...

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Autores principales:	Han Wang, Beibei Chen, Man He, Xiaoxiao Yu, Bin Hu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/51e033f6881b4a35a681636146475595
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spelling	oai:doaj.org-article:51e033f6881b4a35a6816361464755952021-12-02T16:06:48ZSelenocystine against methyl mercury cytotoxicity in HepG2 cells10.1038/s41598-017-00231-72045-2322https://doaj.org/article/51e033f6881b4a35a6816361464755952017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00231-7https://doaj.org/toc/2045-2322Abstract Methyl mercury (MeHg) is a highly toxic substance and the effect of selenium against MeHg toxicity is a hot topic. Until now, no related works have been reported from the view of the point of elemental speciation which is promising to study the mechanism at the molecular level. In this work, to reveal the effect of selenocystine (SeCys2) against MeHg cytotoxicity in HepG2 cells, a comprehensive analytical platform for speciation study of mercury and selenium in MeHg incubated or MeHg and SeCys2 co-incubated HepG2 cells was developed by integrating liquid chromatography (LC) - inductively coupled plasma mass spectrometry (ICP-MS) hyphenated techniques and chip-based pretreatment method. Interesting phenomenon was found that the co-incubation of MeHg with SeCys2 promoted the uptake of MeHg in HepG2 cells, but reduced the cytotoxicity of MeHg. Results obtained by ICP-MS based hyphenated techniques revealed a possible pathway for the incorporation and excretion of mercury species with the coexistence of SeCys2. The formation of MeHg and SeCys2 aggregation promotes the uptake of MeHg; majority of MeHg transforms into small molecular complexes (MeHg-glutathione (GSH) and MeHg-cysteine (Cys)) in HepG2 cells; and MeHg-GSH is the elimination species which results in reducing the cytotoxicity of MeHg.Han WangBeibei ChenMan HeXiaoxiao YuBin HuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Han Wang Beibei Chen Man He Xiaoxiao Yu Bin Hu Selenocystine against methyl mercury cytotoxicity in HepG2 cells
description	Abstract Methyl mercury (MeHg) is a highly toxic substance and the effect of selenium against MeHg toxicity is a hot topic. Until now, no related works have been reported from the view of the point of elemental speciation which is promising to study the mechanism at the molecular level. In this work, to reveal the effect of selenocystine (SeCys2) against MeHg cytotoxicity in HepG2 cells, a comprehensive analytical platform for speciation study of mercury and selenium in MeHg incubated or MeHg and SeCys2 co-incubated HepG2 cells was developed by integrating liquid chromatography (LC) - inductively coupled plasma mass spectrometry (ICP-MS) hyphenated techniques and chip-based pretreatment method. Interesting phenomenon was found that the co-incubation of MeHg with SeCys2 promoted the uptake of MeHg in HepG2 cells, but reduced the cytotoxicity of MeHg. Results obtained by ICP-MS based hyphenated techniques revealed a possible pathway for the incorporation and excretion of mercury species with the coexistence of SeCys2. The formation of MeHg and SeCys2 aggregation promotes the uptake of MeHg; majority of MeHg transforms into small molecular complexes (MeHg-glutathione (GSH) and MeHg-cysteine (Cys)) in HepG2 cells; and MeHg-GSH is the elimination species which results in reducing the cytotoxicity of MeHg.
format	article
author	Han Wang Beibei Chen Man He Xiaoxiao Yu Bin Hu
author_facet	Han Wang Beibei Chen Man He Xiaoxiao Yu Bin Hu
author_sort	Han Wang
title	Selenocystine against methyl mercury cytotoxicity in HepG2 cells
title_short	Selenocystine against methyl mercury cytotoxicity in HepG2 cells
title_full	Selenocystine against methyl mercury cytotoxicity in HepG2 cells
title_fullStr	Selenocystine against methyl mercury cytotoxicity in HepG2 cells
title_full_unstemmed	Selenocystine against methyl mercury cytotoxicity in HepG2 cells
title_sort	selenocystine against methyl mercury cytotoxicity in hepg2 cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/51e033f6881b4a35a681636146475595
work_keys_str_mv	AT hanwang selenocystineagainstmethylmercurycytotoxicityinhepg2cells AT beibeichen selenocystineagainstmethylmercurycytotoxicityinhepg2cells AT manhe selenocystineagainstmethylmercurycytotoxicityinhepg2cells AT xiaoxiaoyu selenocystineagainstmethylmercurycytotoxicityinhepg2cells AT binhu selenocystineagainstmethylmercurycytotoxicityinhepg2cells
_version_	1718384840636628992

Selenocystine against methyl mercury cytotoxicity in HepG2 cells

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