Molecular epidemiology of influenza A(H1N1)pdm09 viruses from Pakistan in 2009-2010.

<h4>Background</h4>In early 2009, a novel influenza A(H1N1) virus that emerged in Mexico and United States rapidly disseminated worldwide. The spread of this virus caused considerable morbidity with over 18000 recorded deaths. The new virus was found to be a reassortant containing gene s...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Uzma Bashir Aamir, Nazish Badar, Muhammad Rashid Mehmood, Nadia Nisar, Rana Muhammad Suleman, Shehzad Shaukat, Salman Sharif, Jaleel Kamran, Syed Sohail Zahoor Zaidi, Birjees Mazher Kazi, Larisa Gubareva, Xiyan Xu, Rebecca Garten, Alexander Klimov
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2012
Materias:
R
Q
Acceso en línea:https://doaj.org/article/51e3de8159754548a91206f0e3b517b9
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:51e3de8159754548a91206f0e3b517b9
record_format dspace
spelling oai:doaj.org-article:51e3de8159754548a91206f0e3b517b92021-11-18T07:08:17ZMolecular epidemiology of influenza A(H1N1)pdm09 viruses from Pakistan in 2009-2010.1932-620310.1371/journal.pone.0041866https://doaj.org/article/51e3de8159754548a91206f0e3b517b92012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22916112/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>In early 2009, a novel influenza A(H1N1) virus that emerged in Mexico and United States rapidly disseminated worldwide. The spread of this virus caused considerable morbidity with over 18000 recorded deaths. The new virus was found to be a reassortant containing gene segments from human, avian and swine influenza viruses.<h4>Methods/results</h4>The first case of human infection with A(H1N1)pdm09 in Pakistan was detected on 18(th) June 2009. Since then, 262 laboratory-confirmed cases have been detected during various outbreaks with 29 deaths (as of 31(st) August 2010). The peak of the epidemic was observed in December with over 51% of total respiratory cases positive for influenza. Representative isolates from Pakistan viruses were sequenced and analyzed antigenically. Sequence analysis of genes coding for surface glycoproteins HA and NA showed high degree of high levels of sequence identity with corresponding genes of regional viruses circulating South East Asia. All tested viruses were sensitive to Oseltamivir in the Neuraminidase Inhibition assays.<h4>Conclusions</h4>Influenza A(H1N1)pdm09 viruses from Pakistan form a homogenous group of viruses. Their HA genes belong to clade 7 and show antigenic profile similar to the vaccine strain A/California/07/2009. These isolates do not show any amino acid changes indicative of high pathogenicity and virulence. It is imperative to continue monitoring of these viruses for identification of potential variants of high virulence or drug resistance.Uzma Bashir AamirNazish BadarMuhammad Rashid MehmoodNadia NisarRana Muhammad SulemanShehzad ShaukatSalman SharifJaleel KamranSyed Sohail Zahoor ZaidiBirjees Mazher KaziLarisa GubarevaXiyan XuRebecca GartenAlexander KlimovPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e41866 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Uzma Bashir Aamir
Nazish Badar
Muhammad Rashid Mehmood
Nadia Nisar
Rana Muhammad Suleman
Shehzad Shaukat
Salman Sharif
Jaleel Kamran
Syed Sohail Zahoor Zaidi
Birjees Mazher Kazi
Larisa Gubareva
Xiyan Xu
Rebecca Garten
Alexander Klimov
Molecular epidemiology of influenza A(H1N1)pdm09 viruses from Pakistan in 2009-2010.
description <h4>Background</h4>In early 2009, a novel influenza A(H1N1) virus that emerged in Mexico and United States rapidly disseminated worldwide. The spread of this virus caused considerable morbidity with over 18000 recorded deaths. The new virus was found to be a reassortant containing gene segments from human, avian and swine influenza viruses.<h4>Methods/results</h4>The first case of human infection with A(H1N1)pdm09 in Pakistan was detected on 18(th) June 2009. Since then, 262 laboratory-confirmed cases have been detected during various outbreaks with 29 deaths (as of 31(st) August 2010). The peak of the epidemic was observed in December with over 51% of total respiratory cases positive for influenza. Representative isolates from Pakistan viruses were sequenced and analyzed antigenically. Sequence analysis of genes coding for surface glycoproteins HA and NA showed high degree of high levels of sequence identity with corresponding genes of regional viruses circulating South East Asia. All tested viruses were sensitive to Oseltamivir in the Neuraminidase Inhibition assays.<h4>Conclusions</h4>Influenza A(H1N1)pdm09 viruses from Pakistan form a homogenous group of viruses. Their HA genes belong to clade 7 and show antigenic profile similar to the vaccine strain A/California/07/2009. These isolates do not show any amino acid changes indicative of high pathogenicity and virulence. It is imperative to continue monitoring of these viruses for identification of potential variants of high virulence or drug resistance.
format article
author Uzma Bashir Aamir
Nazish Badar
Muhammad Rashid Mehmood
Nadia Nisar
Rana Muhammad Suleman
Shehzad Shaukat
Salman Sharif
Jaleel Kamran
Syed Sohail Zahoor Zaidi
Birjees Mazher Kazi
Larisa Gubareva
Xiyan Xu
Rebecca Garten
Alexander Klimov
author_facet Uzma Bashir Aamir
Nazish Badar
Muhammad Rashid Mehmood
Nadia Nisar
Rana Muhammad Suleman
Shehzad Shaukat
Salman Sharif
Jaleel Kamran
Syed Sohail Zahoor Zaidi
Birjees Mazher Kazi
Larisa Gubareva
Xiyan Xu
Rebecca Garten
Alexander Klimov
author_sort Uzma Bashir Aamir
title Molecular epidemiology of influenza A(H1N1)pdm09 viruses from Pakistan in 2009-2010.
title_short Molecular epidemiology of influenza A(H1N1)pdm09 viruses from Pakistan in 2009-2010.
title_full Molecular epidemiology of influenza A(H1N1)pdm09 viruses from Pakistan in 2009-2010.
title_fullStr Molecular epidemiology of influenza A(H1N1)pdm09 viruses from Pakistan in 2009-2010.
title_full_unstemmed Molecular epidemiology of influenza A(H1N1)pdm09 viruses from Pakistan in 2009-2010.
title_sort molecular epidemiology of influenza a(h1n1)pdm09 viruses from pakistan in 2009-2010.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/51e3de8159754548a91206f0e3b517b9
work_keys_str_mv AT uzmabashiraamir molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT nazishbadar molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT muhammadrashidmehmood molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT nadianisar molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT ranamuhammadsuleman molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT shehzadshaukat molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT salmansharif molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT jaleelkamran molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT syedsohailzahoorzaidi molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT birjeesmazherkazi molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT larisagubareva molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT xiyanxu molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT rebeccagarten molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
AT alexanderklimov molecularepidemiologyofinfluenzaah1n1pdm09virusesfrompakistanin20092010
_version_ 1718423914532569088