STUDY OF THE CHEMICAL COMPOSITION AND <i>ANTIFOULING</i> ACTIVITY OF THE MARINE SPONGE <i>Cliona delitrix</i> EXTRACT

In a field test for antifouling activity the raw extract and the organic fraction obtained from the marine sponge Cliona delitrix, collected at the San Andrés Islas (Colombia, Caribbean Sea), showed activity. The organic fraction was separated by column chromatography to obtain enriched fractions of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Leonardo CASTELLANOS H., Humberto MAYORGA W., Carmenza DUQUE B.
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2010
Materias:	Cliona delitrix lipids fatty acids sterols antifouling Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/51f6d6fe039d44beaa3198a9645c0847
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:51f6d6fe039d44beaa3198a9645c0847
record_format	dspace
spelling	oai:doaj.org-article:51f6d6fe039d44beaa3198a9645c08472021-11-19T04:14:21ZSTUDY OF THE CHEMICAL COMPOSITION AND <i>ANTIFOULING</i> ACTIVITY OF THE MARINE SPONGE <i>Cliona delitrix</i> EXTRACT0121-40042145-2660https://doaj.org/article/51f6d6fe039d44beaa3198a9645c08472010-07-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/6346https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660In a field test for antifouling activity the raw extract and the organic fraction obtained from the marine sponge Cliona delitrix, collected at the San Andrés Islas (Colombia, Caribbean Sea), showed activity. The organic fraction was separated by column chromatography to obtain enriched fractions of glycerides, glycolipids, phospholipids, free fatty acids, fatty acids methyl esters (FAMEs) and monohydroxysterols identified by TLC and dereplication techniques (NMR). The glyceride, glycolipid, and phospholipid fractions were hydrolyzed, and the fatty acids methyl esters obtained, together with the initial fatty acids fraction were converted into their methyl esters and analyzed by HRGC-MS. In order to locate unsaturations and alkyl branches in fatty acids, their methyl esters derivates were transformed to the corresponding pyrrolidides and subsequently analyzed by HRGC-MS. The identification of the marifatty acids was carried out using their ECL value as methyl esters, and particularly the study of their pyrrolidide spectra was used to locate unsaturations and alkyl branch positions. The analysis allowed the identification of 89 fatty acids. The main acids include the new 7,10,12,14-icosatetraenoic acid, as well as 5,9-hexacosadienoic acid and hexadecanoic acid. Additionally, 19 monohydroxysterols were identified by HRGC-MS and NMR 1H. Cholestanol and clionasterol were the most abundant compounds and the stanols presence (near to 30%) was not previously reported for this genus.Leonardo CASTELLANOS H.Humberto MAYORGA W.Carmenza DUQUE B.Universidad de AntioquiaarticleCliona delitrixlipidsfatty acidssterolsantifoulingFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 17, Iss 2 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cliona delitrix lipids fatty acids sterols antifouling Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Cliona delitrix lipids fatty acids sterols antifouling Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Leonardo CASTELLANOS H. Humberto MAYORGA W. Carmenza DUQUE B. STUDY OF THE CHEMICAL COMPOSITION AND <i>ANTIFOULING</i> ACTIVITY OF THE MARINE SPONGE <i>Cliona delitrix</i> EXTRACT
description	In a field test for antifouling activity the raw extract and the organic fraction obtained from the marine sponge Cliona delitrix, collected at the San Andrés Islas (Colombia, Caribbean Sea), showed activity. The organic fraction was separated by column chromatography to obtain enriched fractions of glycerides, glycolipids, phospholipids, free fatty acids, fatty acids methyl esters (FAMEs) and monohydroxysterols identified by TLC and dereplication techniques (NMR). The glyceride, glycolipid, and phospholipid fractions were hydrolyzed, and the fatty acids methyl esters obtained, together with the initial fatty acids fraction were converted into their methyl esters and analyzed by HRGC-MS. In order to locate unsaturations and alkyl branches in fatty acids, their methyl esters derivates were transformed to the corresponding pyrrolidides and subsequently analyzed by HRGC-MS. The identification of the marifatty acids was carried out using their ECL value as methyl esters, and particularly the study of their pyrrolidide spectra was used to locate unsaturations and alkyl branch positions. The analysis allowed the identification of 89 fatty acids. The main acids include the new 7,10,12,14-icosatetraenoic acid, as well as 5,9-hexacosadienoic acid and hexadecanoic acid. Additionally, 19 monohydroxysterols were identified by HRGC-MS and NMR 1H. Cholestanol and clionasterol were the most abundant compounds and the stanols presence (near to 30%) was not previously reported for this genus.
format	article
author	Leonardo CASTELLANOS H. Humberto MAYORGA W. Carmenza DUQUE B.
author_facet	Leonardo CASTELLANOS H. Humberto MAYORGA W. Carmenza DUQUE B.
author_sort	Leonardo CASTELLANOS H.
title	STUDY OF THE CHEMICAL COMPOSITION AND <i>ANTIFOULING</i> ACTIVITY OF THE MARINE SPONGE <i>Cliona delitrix</i> EXTRACT
title_short	STUDY OF THE CHEMICAL COMPOSITION AND <i>ANTIFOULING</i> ACTIVITY OF THE MARINE SPONGE <i>Cliona delitrix</i> EXTRACT
title_full	STUDY OF THE CHEMICAL COMPOSITION AND <i>ANTIFOULING</i> ACTIVITY OF THE MARINE SPONGE <i>Cliona delitrix</i> EXTRACT
title_fullStr	STUDY OF THE CHEMICAL COMPOSITION AND <i>ANTIFOULING</i> ACTIVITY OF THE MARINE SPONGE <i>Cliona delitrix</i> EXTRACT
title_full_unstemmed	STUDY OF THE CHEMICAL COMPOSITION AND <i>ANTIFOULING</i> ACTIVITY OF THE MARINE SPONGE <i>Cliona delitrix</i> EXTRACT
title_sort	study of the chemical composition and <i>antifouling</i> activity of the marine sponge <i>cliona delitrix</i> extract
publisher	Universidad de Antioquia
publishDate	2010
url	https://doaj.org/article/51f6d6fe039d44beaa3198a9645c0847
work_keys_str_mv	AT leonardocastellanosh studyofthechemicalcompositionandiantifoulingiactivityofthemarinespongeiclionadelitrixiextract AT humbertomayorgaw studyofthechemicalcompositionandiantifoulingiactivityofthemarinespongeiclionadelitrixiextract AT carmenzaduqueb studyofthechemicalcompositionandiantifoulingiactivityofthemarinespongeiclionadelitrixiextract
_version_	1718420495270936576

STUDY OF THE CHEMICAL COMPOSITION AND <i>ANTIFOULING</i> ACTIVITY OF THE MARINE SPONGE <i>Cliona delitrix</i> EXTRACT

Ejemplares similares