One-Step Preparation of an AgNP-nHA@RGO Three-Dimensional Porous Scaffold and Its Application in Infected Bone Defect Treatment

Weizong Weng,1,2,* Xiaoqun Li,1,* Wei Nie,3 Haoyuan Liu,2 Shanshan Liu,2 Jianming Huang,2 Qirong Zhou,1 Jia He,1 Jiacan Su,1 Zhifeng Dong,4 Dongliang Wang5 1Department of Orthopeadics, Changhai Hospital affiliated to the Second Military Medical University, Shanghai 200433, People’s Republi...

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Autores principales:	Weng W, Li X, Nie W, Liu H, Liu S, Huang J, Zhou Q, He J, Su J, Dong Z, Wang D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	biofilm graphene oxide hydroxyapatite infected bone defect scaffold silver nanoparticles three-dimensional Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/51feb113e31042e29616dfbaf570a701
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spelling	oai:doaj.org-article:51feb113e31042e29616dfbaf570a7012021-12-02T15:31:13ZOne-Step Preparation of an AgNP-nHA@RGO Three-Dimensional Porous Scaffold and Its Application in Infected Bone Defect Treatment1178-2013https://doaj.org/article/51feb113e31042e29616dfbaf570a7012020-07-01T00:00:00Zhttps://www.dovepress.com/one-step-preparation-of-an-agnp-nhargo-three-dimensional-porous-scaffo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Weizong Weng,1,2,* Xiaoqun Li,1,* Wei Nie,3 Haoyuan Liu,2 Shanshan Liu,2 Jianming Huang,2 Qirong Zhou,1 Jia He,1 Jiacan Su,1 Zhifeng Dong,4 Dongliang Wang5 1Department of Orthopeadics, Changhai Hospital affiliated to the Second Military Medical University, Shanghai 200433, People’s Republic of China; 2Orthopaedics Department, Chenggong Hospital Affilaited to Xiamen University, Xiamen 361000, People’s Republic of China; 3College of Chemistry, Chemical Engineering and Biotechnology, State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, People’s Republic of China; 4Department of Cardiology, Shanghai Sixth Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200233, People’s Republic of China; 5Orthopeadics Department, Xinhua Hospital Affiliated to the Shanghai Jiaotong University, Shanghai 200433, People’s Republic of China*These authors contributed equally to this workCorrespondence: Dongliang Wang; Weizong Weng Email wangdongliang@xinhuanmed.com.cn; drwengweizong@163.comBackground: Bactericidal capacity, durable inhibition of biofilm formation, and a three-dimensional (3D) porous structure are the emphases of infected bone defect (IBD) treatment via local scaffold implantation strategy.Purpose: In this study, silver nanoparticle (AgNP)-loaded nano-hydroxyapatite (nHA)@ reduced graphene oxide (RGO) 3D scaffolds (AHRG scaffolds) were designed to alleviate bone infection, inhibit biofilm formation, and promote bone repair through the synergistic effects of AgNPs, RGO, and nHA.Materials and Methods: AHRGs were prepared using a one-step preparation method, to create a 3D porous scaffold to facilitate a uniform distribution of AgNPs and nHA. Methicillin-resistant Staphylococcus aureus (MRSA) was used as a model-resistant bacterium, and the effects of different silver loadings on the antimicrobial activity and cytocompatibility of materials were evaluated. Finally, a rabbit IBD model was used to evaluate the therapeutic effect of the AHRG scaffold in vivo.Results: The results showed successful synthesis of the AHRG scaffold. The ideal 3D porous structure was verified using scanning electron microscopy and transmission electron microscopy, and X-ray photoelectron spectroscopy and selected area electron diffraction measurements revealed uniform distributions of AgNP and nHA. In vitro antibacterial and cytocompatibility indicated that the 4% AHRG scaffolds possessed the most favorable balance of bactericidal properties and cytocompatibility. In vivo evaluation of the IBD model showed promising treatment efficacy of AHRG scaffolds.Conclusion: The as-fabricated AHRG scaffolds effectively eliminated infection and inhibited biofilm formation. IBD repair was facilitated by the bactericidal properties and 3D porous structure of the AHRG scaffold, suggesting its potential in the treatment of IBDs.Keywords: biofilm, graphene oxide, hydroxyapatite, infected bone defect, scaffold, silver nanoparticles, three-dimensionalWeng WLi XNie WLiu HLiu SHuang JZhou QHe JSu JDong ZWang DDove Medical Pressarticlebiofilmgraphene oxidehydroxyapatiteinfected bone defectscaffoldsilver nanoparticlesthree-dimensionalMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 5027-5042 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	biofilm graphene oxide hydroxyapatite infected bone defect scaffold silver nanoparticles three-dimensional Medicine (General) R5-920
spellingShingle	biofilm graphene oxide hydroxyapatite infected bone defect scaffold silver nanoparticles three-dimensional Medicine (General) R5-920 Weng W Li X Nie W Liu H Liu S Huang J Zhou Q He J Su J Dong Z Wang D One-Step Preparation of an AgNP-nHA@RGO Three-Dimensional Porous Scaffold and Its Application in Infected Bone Defect Treatment
description	Weizong Weng,1,2,* Xiaoqun Li,1,* Wei Nie,3 Haoyuan Liu,2 Shanshan Liu,2 Jianming Huang,2 Qirong Zhou,1 Jia He,1 Jiacan Su,1 Zhifeng Dong,4 Dongliang Wang5 1Department of Orthopeadics, Changhai Hospital affiliated to the Second Military Medical University, Shanghai 200433, People’s Republic of China; 2Orthopaedics Department, Chenggong Hospital Affilaited to Xiamen University, Xiamen 361000, People’s Republic of China; 3College of Chemistry, Chemical Engineering and Biotechnology, State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, People’s Republic of China; 4Department of Cardiology, Shanghai Sixth Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200233, People’s Republic of China; 5Orthopeadics Department, Xinhua Hospital Affiliated to the Shanghai Jiaotong University, Shanghai 200433, People’s Republic of China*These authors contributed equally to this workCorrespondence: Dongliang Wang; Weizong Weng Email wangdongliang@xinhuanmed.com.cn; drwengweizong@163.comBackground: Bactericidal capacity, durable inhibition of biofilm formation, and a three-dimensional (3D) porous structure are the emphases of infected bone defect (IBD) treatment via local scaffold implantation strategy.Purpose: In this study, silver nanoparticle (AgNP)-loaded nano-hydroxyapatite (nHA)@ reduced graphene oxide (RGO) 3D scaffolds (AHRG scaffolds) were designed to alleviate bone infection, inhibit biofilm formation, and promote bone repair through the synergistic effects of AgNPs, RGO, and nHA.Materials and Methods: AHRGs were prepared using a one-step preparation method, to create a 3D porous scaffold to facilitate a uniform distribution of AgNPs and nHA. Methicillin-resistant Staphylococcus aureus (MRSA) was used as a model-resistant bacterium, and the effects of different silver loadings on the antimicrobial activity and cytocompatibility of materials were evaluated. Finally, a rabbit IBD model was used to evaluate the therapeutic effect of the AHRG scaffold in vivo.Results: The results showed successful synthesis of the AHRG scaffold. The ideal 3D porous structure was verified using scanning electron microscopy and transmission electron microscopy, and X-ray photoelectron spectroscopy and selected area electron diffraction measurements revealed uniform distributions of AgNP and nHA. In vitro antibacterial and cytocompatibility indicated that the 4% AHRG scaffolds possessed the most favorable balance of bactericidal properties and cytocompatibility. In vivo evaluation of the IBD model showed promising treatment efficacy of AHRG scaffolds.Conclusion: The as-fabricated AHRG scaffolds effectively eliminated infection and inhibited biofilm formation. IBD repair was facilitated by the bactericidal properties and 3D porous structure of the AHRG scaffold, suggesting its potential in the treatment of IBDs.Keywords: biofilm, graphene oxide, hydroxyapatite, infected bone defect, scaffold, silver nanoparticles, three-dimensional
format	article
author	Weng W Li X Nie W Liu H Liu S Huang J Zhou Q He J Su J Dong Z Wang D
author_facet	Weng W Li X Nie W Liu H Liu S Huang J Zhou Q He J Su J Dong Z Wang D
author_sort	Weng W
title	One-Step Preparation of an AgNP-nHA@RGO Three-Dimensional Porous Scaffold and Its Application in Infected Bone Defect Treatment
title_short	One-Step Preparation of an AgNP-nHA@RGO Three-Dimensional Porous Scaffold and Its Application in Infected Bone Defect Treatment
title_full	One-Step Preparation of an AgNP-nHA@RGO Three-Dimensional Porous Scaffold and Its Application in Infected Bone Defect Treatment
title_fullStr	One-Step Preparation of an AgNP-nHA@RGO Three-Dimensional Porous Scaffold and Its Application in Infected Bone Defect Treatment
title_full_unstemmed	One-Step Preparation of an AgNP-nHA@RGO Three-Dimensional Porous Scaffold and Its Application in Infected Bone Defect Treatment
title_sort	one-step preparation of an agnp-nha@rgo three-dimensional porous scaffold and its application in infected bone defect treatment
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/51feb113e31042e29616dfbaf570a701
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One-Step Preparation of an AgNP-nHA@RGO Three-Dimensional Porous Scaffold and Its Application in Infected Bone Defect Treatment

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