MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A

Objective. The study was designed to evaluate the underlying mechanism of microRNA-139-5p in breast cancer (BC). Methods. Expression statuses of microRNA-139-5p and MEX3A were measured by qRT-PCR and western blotting. The anticancer effect of microRNA-139-5p in vitro was tested by a set of assays. I...

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Autores principales:	Jian Chu, Tangya Li, Lei Li, Huiwen Fan
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Computer applications to medicine. Medical informatics R858-859.7
Acceso en línea:	https://doaj.org/article/5200c170630344e5b065d78421db7d6c
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spelling	oai:doaj.org-article:5200c170630344e5b065d78421db7d6c2021-11-15T01:19:48ZMicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A1748-671810.1155/2021/6591541https://doaj.org/article/5200c170630344e5b065d78421db7d6c2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/6591541https://doaj.org/toc/1748-6718Objective. The study was designed to evaluate the underlying mechanism of microRNA-139-5p in breast cancer (BC). Methods. Expression statuses of microRNA-139-5p and MEX3A were measured by qRT-PCR and western blotting. The anticancer effect of microRNA-139-5p in vitro was tested by a set of assays. Interaction between microRNA-139-5p and MEX3A was validated by dual-luciferase detection. Results. MicroRNA-139-5p expression in BC cells was obviously low, while MEX3A was significantly overexpressed. MicroRNA-139-5p restrained proliferative, invasive, and migratory abilities of BC cells and increased apoptosis level of BC cells, while MEX3A exerted a promoting effect on BC cell growth. Dual-luciferase reporter detection confirmed that microRNA-139-5p bound to MEX3A 3′-UTR. Conclusions. MicroRNA-139-5p inhibited the development of BC by targeting MEX3A. MicroRNA-139-5p/MEX3A may be a target for BC therapy.Jian ChuTangya LiLei LiHuiwen FanHindawi LimitedarticleComputer applications to medicine. Medical informaticsR858-859.7ENComputational and Mathematical Methods in Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Computer applications to medicine. Medical informatics R858-859.7
spellingShingle	Computer applications to medicine. Medical informatics R858-859.7 Jian Chu Tangya Li Lei Li Huiwen Fan MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A
description	Objective. The study was designed to evaluate the underlying mechanism of microRNA-139-5p in breast cancer (BC). Methods. Expression statuses of microRNA-139-5p and MEX3A were measured by qRT-PCR and western blotting. The anticancer effect of microRNA-139-5p in vitro was tested by a set of assays. Interaction between microRNA-139-5p and MEX3A was validated by dual-luciferase detection. Results. MicroRNA-139-5p expression in BC cells was obviously low, while MEX3A was significantly overexpressed. MicroRNA-139-5p restrained proliferative, invasive, and migratory abilities of BC cells and increased apoptosis level of BC cells, while MEX3A exerted a promoting effect on BC cell growth. Dual-luciferase reporter detection confirmed that microRNA-139-5p bound to MEX3A 3′-UTR. Conclusions. MicroRNA-139-5p inhibited the development of BC by targeting MEX3A. MicroRNA-139-5p/MEX3A may be a target for BC therapy.
format	article
author	Jian Chu Tangya Li Lei Li Huiwen Fan
author_facet	Jian Chu Tangya Li Lei Li Huiwen Fan
author_sort	Jian Chu
title	MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A
title_short	MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A
title_full	MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A
title_fullStr	MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A
title_full_unstemmed	MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A
title_sort	microrna-139-5p suppresses cell malignant behaviors in breast cancer through targeting mex3a
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/5200c170630344e5b065d78421db7d6c
work_keys_str_mv	AT jianchu microrna1395psuppressescellmalignantbehaviorsinbreastcancerthroughtargetingmex3a AT tangyali microrna1395psuppressescellmalignantbehaviorsinbreastcancerthroughtargetingmex3a AT leili microrna1395psuppressescellmalignantbehaviorsinbreastcancerthroughtargetingmex3a AT huiwenfan microrna1395psuppressescellmalignantbehaviorsinbreastcancerthroughtargetingmex3a
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MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A

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