Microfluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc

Intervertebral disc (IVD) degeneration is a major cause of low back pain (LBP) in the lumbar spine. This phenomenon is caused by several processes, including matrix degradation in IVD tissues, which is mediated by matrix metalloproteinases (MMPs) and inflammatory responses, which can be mediated by...

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Autores principales:	An-Gi Kim, Tae-Won Kim, Woo-Keun Kwon, Kwang-Ho Lee, Sehoon Jeong, Min-Ho Hwang, Hyuk Choi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microfluidic chip electrical stimulation low constant-current stimulation intervertebral disc degeneration inflammation Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/5202d49da5124b0692bdada96712d4bf
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spelling	oai:doaj.org-article:5202d49da5124b0692bdada96712d4bf2021-11-25T18:22:49ZMicrofluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc10.3390/mi121112912072-666Xhttps://doaj.org/article/5202d49da5124b0692bdada96712d4bf2021-10-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1291https://doaj.org/toc/2072-666XIntervertebral disc (IVD) degeneration is a major cause of low back pain (LBP) in the lumbar spine. This phenomenon is caused by several processes, including matrix degradation in IVD tissues, which is mediated by matrix metalloproteinases (MMPs) and inflammatory responses, which can be mediated by interactions among immune cells, such as macrophages and IVD cells. In particular, interleukin (IL)-1 beta (β), which is a master regulator secreted by macrophages, mediates the inflammatory response in nucleus pulposus cells (NP) and plays a significant role in the development or progression of diseases. In this study, we developed a custom electrical stimulation (ES) platform that can apply low-constant-current stimulation (LCCS) signals to microfluidic chips. Using this platform, we examined the effects of LCCS on IL-1β-mediated inflammatory NP cells, administered at various currents (5, 10, 20, 50, and 100 μA at 200 Hz). Our results showed that the inflammatory response, induced by IL-1β in human NP cells, was successfully established. Furthermore, 5, 10, 20, and 100 μA LCCS positively modulated inflamed human NP cells’ morphological phenotype and kinetic properties. LCCS could affect the treatment of degenerative diseases, revealing the applicability of the LCCS platform for basic research of electroceuticals.An-Gi KimTae-Won KimWoo-Keun KwonKwang-Ho LeeSehoon JeongMin-Ho HwangHyuk ChoiMDPI AGarticlemicrofluidic chipelectrical stimulationlow constant-current stimulationintervertebral disc degenerationinflammationMechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1291, p 1291 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microfluidic chip electrical stimulation low constant-current stimulation intervertebral disc degeneration inflammation Mechanical engineering and machinery TJ1-1570
spellingShingle	microfluidic chip electrical stimulation low constant-current stimulation intervertebral disc degeneration inflammation Mechanical engineering and machinery TJ1-1570 An-Gi Kim Tae-Won Kim Woo-Keun Kwon Kwang-Ho Lee Sehoon Jeong Min-Ho Hwang Hyuk Choi Microfluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc
description	Intervertebral disc (IVD) degeneration is a major cause of low back pain (LBP) in the lumbar spine. This phenomenon is caused by several processes, including matrix degradation in IVD tissues, which is mediated by matrix metalloproteinases (MMPs) and inflammatory responses, which can be mediated by interactions among immune cells, such as macrophages and IVD cells. In particular, interleukin (IL)-1 beta (β), which is a master regulator secreted by macrophages, mediates the inflammatory response in nucleus pulposus cells (NP) and plays a significant role in the development or progression of diseases. In this study, we developed a custom electrical stimulation (ES) platform that can apply low-constant-current stimulation (LCCS) signals to microfluidic chips. Using this platform, we examined the effects of LCCS on IL-1β-mediated inflammatory NP cells, administered at various currents (5, 10, 20, 50, and 100 μA at 200 Hz). Our results showed that the inflammatory response, induced by IL-1β in human NP cells, was successfully established. Furthermore, 5, 10, 20, and 100 μA LCCS positively modulated inflamed human NP cells’ morphological phenotype and kinetic properties. LCCS could affect the treatment of degenerative diseases, revealing the applicability of the LCCS platform for basic research of electroceuticals.
format	article
author	An-Gi Kim Tae-Won Kim Woo-Keun Kwon Kwang-Ho Lee Sehoon Jeong Min-Ho Hwang Hyuk Choi
author_facet	An-Gi Kim Tae-Won Kim Woo-Keun Kwon Kwang-Ho Lee Sehoon Jeong Min-Ho Hwang Hyuk Choi
author_sort	An-Gi Kim
title	Microfluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc
title_short	Microfluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc
title_full	Microfluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc
title_fullStr	Microfluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc
title_full_unstemmed	Microfluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc
title_sort	microfluidic chip with low constant-current stimulation (lccs) platform: human nucleus pulposus degeneration in vitro model for symptomatic intervertebral disc
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5202d49da5124b0692bdada96712d4bf
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Microfluidic Chip with Low Constant-Current Stimulation (LCCS) Platform: Human Nucleus Pulposus Degeneration In Vitro Model for Symptomatic Intervertebral Disc

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