A cross-sectional study on the association of single nucleotide polymorphism of leptin receptor (Gln223Arg) and insulin resistance in gestational diabetes mellitus

Background: The role of genetics in pathophysiology of gestational diabetes mellitus (GDM) is least explored. Objective of the study is to find the association of leptin receptor (LEPR) gene polymorphism with GDM and its role in altered leptin levels, insulin resistance, and dyslipidemia in GDM. Met...

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Autores principales:	Usha Adiga, Sachidananda Adiga, P.B. Nandit, Lakshmi Manjeera, Aparna Rao, Abdul-Kareem Mohammed Ghilan, Atif Abdulwahab A. Oyouni, Yousef M. Hawsawi, Abdulrahman Theyab, Mohammad Algahtani, Othman R. Alzahrani, Ravi Mundugaru
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Gestational diabetes mellitus Insulin resistance Hardy-weinberg equillibrium Leptin Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/520cf82597624d3c83bd43718fa462f5
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spelling	oai:doaj.org-article:520cf82597624d3c83bd43718fa462f52021-11-10T04:20:59ZA cross-sectional study on the association of single nucleotide polymorphism of leptin receptor (Gln223Arg) and insulin resistance in gestational diabetes mellitus1018-364710.1016/j.jksus.2021.101662https://doaj.org/article/520cf82597624d3c83bd43718fa462f52022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721003244https://doaj.org/toc/1018-3647Background: The role of genetics in pathophysiology of gestational diabetes mellitus (GDM) is least explored. Objective of the study is to find the association of leptin receptor (LEPR) gene polymorphism with GDM and its role in altered leptin levels, insulin resistance, and dyslipidemia in GDM. Methods: Hundred GDM patients and an equal number of BMI and gestational age matched normal glucose tolerant pregnant women were recruited as cases and controls. Five milliliters of venous blood was drawn for biochemical and genetic analysis. Genotyping of LEPR (Gln223Arg) was performed by PCR-RFLP. Fasting blood sugar, leptin, insulin, C-peptide, and lipid profile estimations were done. Various insulin resistance models were constructed using suitable formulae. The statistical analysis was performed using SPSS 23.0 software. Results: There was no significant association found between LEPR gene polymorphism with leptin levels and insulin resistance in GDM. However, Odd’s ratio showed that individuals with the “A” allele were at 1.25 times higher risk of developing GDM. HOMA B-cell significantly varied among LEPR genotypes (p < 0.0001), values being double in AA genotype, compared to AG (p < 0.05), ten times higher in AA compared to GG (p < 0.0001). There was no significant deviation of the genotype frequency distributions for rs1137101 variants from HWE in GDM cases, suggesting that alleles were in equilibrium. Conclusion: The study established a cycle of gene polymorphism altering leptin levels which in turn can alter insulin secretion and insulin resistance, contributing to dyslipidemia of pregnancy as well as gestational diabetes.Usha AdigaSachidananda AdigaP.B. NanditLakshmi ManjeeraAparna RaoAbdul-Kareem Mohammed GhilanAtif Abdulwahab A. OyouniYousef M. HawsawiAbdulrahman TheyabMohammad AlgahtaniOthman R. AlzahraniRavi MundugaruElsevierarticleGestational diabetes mellitusInsulin resistanceHardy-weinberg equillibriumLeptinScience (General)Q1-390ENJournal of King Saud University: Science, Vol 34, Iss 1, Pp 101662- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gestational diabetes mellitus Insulin resistance Hardy-weinberg equillibrium Leptin Science (General) Q1-390
spellingShingle	Gestational diabetes mellitus Insulin resistance Hardy-weinberg equillibrium Leptin Science (General) Q1-390 Usha Adiga Sachidananda Adiga P.B. Nandit Lakshmi Manjeera Aparna Rao Abdul-Kareem Mohammed Ghilan Atif Abdulwahab A. Oyouni Yousef M. Hawsawi Abdulrahman Theyab Mohammad Algahtani Othman R. Alzahrani Ravi Mundugaru A cross-sectional study on the association of single nucleotide polymorphism of leptin receptor (Gln223Arg) and insulin resistance in gestational diabetes mellitus
description	Background: The role of genetics in pathophysiology of gestational diabetes mellitus (GDM) is least explored. Objective of the study is to find the association of leptin receptor (LEPR) gene polymorphism with GDM and its role in altered leptin levels, insulin resistance, and dyslipidemia in GDM. Methods: Hundred GDM patients and an equal number of BMI and gestational age matched normal glucose tolerant pregnant women were recruited as cases and controls. Five milliliters of venous blood was drawn for biochemical and genetic analysis. Genotyping of LEPR (Gln223Arg) was performed by PCR-RFLP. Fasting blood sugar, leptin, insulin, C-peptide, and lipid profile estimations were done. Various insulin resistance models were constructed using suitable formulae. The statistical analysis was performed using SPSS 23.0 software. Results: There was no significant association found between LEPR gene polymorphism with leptin levels and insulin resistance in GDM. However, Odd’s ratio showed that individuals with the “A” allele were at 1.25 times higher risk of developing GDM. HOMA B-cell significantly varied among LEPR genotypes (p < 0.0001), values being double in AA genotype, compared to AG (p < 0.05), ten times higher in AA compared to GG (p < 0.0001). There was no significant deviation of the genotype frequency distributions for rs1137101 variants from HWE in GDM cases, suggesting that alleles were in equilibrium. Conclusion: The study established a cycle of gene polymorphism altering leptin levels which in turn can alter insulin secretion and insulin resistance, contributing to dyslipidemia of pregnancy as well as gestational diabetes.
format	article
author	Usha Adiga Sachidananda Adiga P.B. Nandit Lakshmi Manjeera Aparna Rao Abdul-Kareem Mohammed Ghilan Atif Abdulwahab A. Oyouni Yousef M. Hawsawi Abdulrahman Theyab Mohammad Algahtani Othman R. Alzahrani Ravi Mundugaru
author_facet	Usha Adiga Sachidananda Adiga P.B. Nandit Lakshmi Manjeera Aparna Rao Abdul-Kareem Mohammed Ghilan Atif Abdulwahab A. Oyouni Yousef M. Hawsawi Abdulrahman Theyab Mohammad Algahtani Othman R. Alzahrani Ravi Mundugaru
author_sort	Usha Adiga
title	A cross-sectional study on the association of single nucleotide polymorphism of leptin receptor (Gln223Arg) and insulin resistance in gestational diabetes mellitus
title_short	A cross-sectional study on the association of single nucleotide polymorphism of leptin receptor (Gln223Arg) and insulin resistance in gestational diabetes mellitus
title_full	A cross-sectional study on the association of single nucleotide polymorphism of leptin receptor (Gln223Arg) and insulin resistance in gestational diabetes mellitus
title_fullStr	A cross-sectional study on the association of single nucleotide polymorphism of leptin receptor (Gln223Arg) and insulin resistance in gestational diabetes mellitus
title_full_unstemmed	A cross-sectional study on the association of single nucleotide polymorphism of leptin receptor (Gln223Arg) and insulin resistance in gestational diabetes mellitus
title_sort	cross-sectional study on the association of single nucleotide polymorphism of leptin receptor (gln223arg) and insulin resistance in gestational diabetes mellitus
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/520cf82597624d3c83bd43718fa462f5
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A cross-sectional study on the association of single nucleotide polymorphism of leptin receptor (Gln223Arg) and insulin resistance in gestational diabetes mellitus

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