The abundance of Akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis

Abstract Akkermansia muciniphila utilises colonic mucin as its substrate. Abundance is reduced in ulcerative colitis (UC), as is the relative proportion of sulphated mucin in the mucus gel layer (MGL). It is unknown if these phenomena are related, however reduced sulphated mucins could contribute to...

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Autores principales:	Helen Earley, Grainne Lennon, Áine Balfe, J. Calvin Coffey, Desmond C. Winter, P. Ronan O’Connell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5213841f65db421d829b47f1c74dd893
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spelling	oai:doaj.org-article:5213841f65db421d829b47f1c74dd8932021-12-02T15:08:31ZThe abundance of Akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis10.1038/s41598-019-51878-32045-2322https://doaj.org/article/5213841f65db421d829b47f1c74dd8932019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51878-3https://doaj.org/toc/2045-2322Abstract Akkermansia muciniphila utilises colonic mucin as its substrate. Abundance is reduced in ulcerative colitis (UC), as is the relative proportion of sulphated mucin in the mucus gel layer (MGL). It is unknown if these phenomena are related, however reduced sulphated mucins could contribute to reduced abundance, owing to a lack of substrate. The aim of this study was to quantify A. muciniphila within the MGL and to relate these findings with markers of inflammation and the relative proportion of sulphomucin present. Colonic biopsies and mucus brushings were obtained from 20 patients with active UC (AC), 14 with quiescent UC (QUC) and 20 healthy controls (HC). A. muciniphila abundance was determined by RT-PCR. High iron diamine alcian-blue staining was performed for histological analysis. Patients with AC had reduced abundance of A. muciniphila compared to HC and QUC. A positive association was found between A. muciniphila abundance and higher percentage of sulphated mucin (ρ 0.546, p = 0.000). Lower abundances of A. muciniphila correlated with higher inflammatory scores (ρ = 0.294 (p = 0.001)). This study confirms an inverse relationship between A. muciniphila and inflammation and a positive association between A. muciniphila abundance and percentage of sulfated mucin in the MGL.Helen EarleyGrainne LennonÁine BalfeJ. Calvin CoffeyDesmond C. WinterP. Ronan O’ConnellNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Helen Earley Grainne Lennon Áine Balfe J. Calvin Coffey Desmond C. Winter P. Ronan O’Connell The abundance of Akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis
description	Abstract Akkermansia muciniphila utilises colonic mucin as its substrate. Abundance is reduced in ulcerative colitis (UC), as is the relative proportion of sulphated mucin in the mucus gel layer (MGL). It is unknown if these phenomena are related, however reduced sulphated mucins could contribute to reduced abundance, owing to a lack of substrate. The aim of this study was to quantify A. muciniphila within the MGL and to relate these findings with markers of inflammation and the relative proportion of sulphomucin present. Colonic biopsies and mucus brushings were obtained from 20 patients with active UC (AC), 14 with quiescent UC (QUC) and 20 healthy controls (HC). A. muciniphila abundance was determined by RT-PCR. High iron diamine alcian-blue staining was performed for histological analysis. Patients with AC had reduced abundance of A. muciniphila compared to HC and QUC. A positive association was found between A. muciniphila abundance and higher percentage of sulphated mucin (ρ 0.546, p = 0.000). Lower abundances of A. muciniphila correlated with higher inflammatory scores (ρ = 0.294 (p = 0.001)). This study confirms an inverse relationship between A. muciniphila and inflammation and a positive association between A. muciniphila abundance and percentage of sulfated mucin in the MGL.
format	article
author	Helen Earley Grainne Lennon Áine Balfe J. Calvin Coffey Desmond C. Winter P. Ronan O’Connell
author_facet	Helen Earley Grainne Lennon Áine Balfe J. Calvin Coffey Desmond C. Winter P. Ronan O’Connell
author_sort	Helen Earley
title	The abundance of Akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis
title_short	The abundance of Akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis
title_full	The abundance of Akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis
title_fullStr	The abundance of Akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis
title_full_unstemmed	The abundance of Akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis
title_sort	abundance of akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/5213841f65db421d829b47f1c74dd893
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The abundance of Akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis

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