Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.

Human bone marrow mesenchymal stem cells (BM-MSC) are multipotent progenitor cells that have transient immunomodulatory properties on Natural Killer (NK) cells, Dendritic Cells (DC), and T cells. This study compared the use of MSC isolated from bone marrow and fetal liver (FL-MSC) to determine which...

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Autores principales:	Massimo Giuliani, Maud Fleury, Amelia Vernochet, Farah Ketroussi, Denis Clay, Bruno Azzarone, Jean Jacques Lataillade, Antoine Durrbach
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/521519455890454288e78fa223c128dc
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spelling	oai:doaj.org-article:521519455890454288e78fa223c128dc2021-11-18T06:53:41ZLong-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.1932-620310.1371/journal.pone.0019988https://doaj.org/article/521519455890454288e78fa223c128dc2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625521/?tool=EBIhttps://doaj.org/toc/1932-6203Human bone marrow mesenchymal stem cells (BM-MSC) are multipotent progenitor cells that have transient immunomodulatory properties on Natural Killer (NK) cells, Dendritic Cells (DC), and T cells. This study compared the use of MSC isolated from bone marrow and fetal liver (FL-MSC) to determine which displayed the most efficient immunosuppressive effects on T cell activation. Although both types of MSC exhibit similar phenotype profile, FL-MSC displays a much more extended in vitro life-span and immunomodulatory properties. When co-cultured with CD3/CD28-stimulated T cells, both BM-MSC and FL-MSC affected T cell proliferation by inhibiting their entry into the cell cycle, by inducing the down-regulation of phospho-retinoblastoma (pRb), cyclins A and D1, as well as up-regulating p27(kip1) expression. The T cell inhibition by MSC was not due to the soluble HLA-G5 isoform, but to the surface expression of HLA-G1, as shown by the need of cell-cell contact and by the use of neutralizing anti-HLA-G antibodies. To note, in a HLA-G-mediated fashion, MSC facilitated the expansion of a CD4(low)/CD8(low) T subset that had decreased secretion of IFN-γ, and an induced secretion of the immunomodulatory cytokine IL-10. Because of their longer lasting in vitro immunosuppressive properties, mainly mediated by HLA-G, and their more efficient induction of IL-10 production and T cell apoptosis, fetal liver MSC could be considered a new tool for MSC therapy to prevent allograft rejection.Massimo GiulianiMaud FleuryAmelia VernochetFarah KetroussiDenis ClayBruno AzzaroneJean Jacques LatailladeAntoine DurrbachPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e19988 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Massimo Giuliani Maud Fleury Amelia Vernochet Farah Ketroussi Denis Clay Bruno Azzarone Jean Jacques Lataillade Antoine Durrbach Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
description	Human bone marrow mesenchymal stem cells (BM-MSC) are multipotent progenitor cells that have transient immunomodulatory properties on Natural Killer (NK) cells, Dendritic Cells (DC), and T cells. This study compared the use of MSC isolated from bone marrow and fetal liver (FL-MSC) to determine which displayed the most efficient immunosuppressive effects on T cell activation. Although both types of MSC exhibit similar phenotype profile, FL-MSC displays a much more extended in vitro life-span and immunomodulatory properties. When co-cultured with CD3/CD28-stimulated T cells, both BM-MSC and FL-MSC affected T cell proliferation by inhibiting their entry into the cell cycle, by inducing the down-regulation of phospho-retinoblastoma (pRb), cyclins A and D1, as well as up-regulating p27(kip1) expression. The T cell inhibition by MSC was not due to the soluble HLA-G5 isoform, but to the surface expression of HLA-G1, as shown by the need of cell-cell contact and by the use of neutralizing anti-HLA-G antibodies. To note, in a HLA-G-mediated fashion, MSC facilitated the expansion of a CD4(low)/CD8(low) T subset that had decreased secretion of IFN-γ, and an induced secretion of the immunomodulatory cytokine IL-10. Because of their longer lasting in vitro immunosuppressive properties, mainly mediated by HLA-G, and their more efficient induction of IL-10 production and T cell apoptosis, fetal liver MSC could be considered a new tool for MSC therapy to prevent allograft rejection.
format	article
author	Massimo Giuliani Maud Fleury Amelia Vernochet Farah Ketroussi Denis Clay Bruno Azzarone Jean Jacques Lataillade Antoine Durrbach
author_facet	Massimo Giuliani Maud Fleury Amelia Vernochet Farah Ketroussi Denis Clay Bruno Azzarone Jean Jacques Lataillade Antoine Durrbach
author_sort	Massimo Giuliani
title	Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
title_short	Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
title_full	Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
title_fullStr	Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
title_full_unstemmed	Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
title_sort	long-lasting inhibitory effects of fetal liver mesenchymal stem cells on t-lymphocyte proliferation.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/521519455890454288e78fa223c128dc
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Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.

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