The selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.

Equipartitioning by chromosome association and copy number correction by DNA amplification are at the heart of the evolutionary success of the selfish yeast 2-micron plasmid. The present analysis reveals frequent plasmid presence near telomeres (TELs) and centromeres (CENs) in mitotic cells, with a...

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Autores principales:	Deepanshu Kumar, Hemant Kumar Prajapati, Anjali Mahilkar, Chien-Hui Ma, Priyanka Mittal, Makkuni Jayaram, Santanu K Ghosh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/5216b172c1834e6cbed9a58322a9cff2
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id	oai:doaj.org-article:5216b172c1834e6cbed9a58322a9cff2
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spelling	oai:doaj.org-article:5216b172c1834e6cbed9a58322a9cff22021-12-02T20:02:56ZThe selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.1553-73901553-740410.1371/journal.pgen.1009660https://doaj.org/article/5216b172c1834e6cbed9a58322a9cff22021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009660https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Equipartitioning by chromosome association and copy number correction by DNA amplification are at the heart of the evolutionary success of the selfish yeast 2-micron plasmid. The present analysis reveals frequent plasmid presence near telomeres (TELs) and centromeres (CENs) in mitotic cells, with a preference towards the former. Inactivation of Cdc14 causes plasmid missegregation, which is correlated to the non-disjunction of TELs (and of rDNA) under this condition. Induced missegregation of chromosome XII, one of the largest yeast chromosomes which harbors the rDNA array and is highly dependent on the condensin complex for proper disjunction, increases 2-micron plasmid missegregation. This is not the case when chromosome III, one of the smallest chromosomes, is forced to missegregate. Plasmid stability decreases when the condensin subunit Brn1 is inactivated. Brn1 is recruited to the plasmid partitioning locus (STB) with the assistance of the plasmid-coded partitioning proteins Rep1 and Rep2. Furthermore, in a dihybrid assay, Brn1 interacts with Rep1-Rep2. Taken together, these findings support a role for condensin and/or condensed chromatin in 2-micron plasmid propagation. They suggest that condensed chromosome loci are among favored sites utilized by the plasmid for its chromosome-associated segregation. By homing to condensed/quiescent chromosome locales, and not over-perturbing genome homeostasis, the plasmid may minimize fitness conflicts with its host. Analogous persistence strategies may be utilized by other extrachromosomal selfish genomes, for example, episomes of mammalian viruses that hitchhike on host chromosomes for their stable maintenance.Deepanshu KumarHemant Kumar PrajapatiAnjali MahilkarChien-Hui MaPriyanka MittalMakkuni JayaramSantanu K GhoshPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 7, p e1009660 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Deepanshu Kumar Hemant Kumar Prajapati Anjali Mahilkar Chien-Hui Ma Priyanka Mittal Makkuni Jayaram Santanu K Ghosh The selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.
description	Equipartitioning by chromosome association and copy number correction by DNA amplification are at the heart of the evolutionary success of the selfish yeast 2-micron plasmid. The present analysis reveals frequent plasmid presence near telomeres (TELs) and centromeres (CENs) in mitotic cells, with a preference towards the former. Inactivation of Cdc14 causes plasmid missegregation, which is correlated to the non-disjunction of TELs (and of rDNA) under this condition. Induced missegregation of chromosome XII, one of the largest yeast chromosomes which harbors the rDNA array and is highly dependent on the condensin complex for proper disjunction, increases 2-micron plasmid missegregation. This is not the case when chromosome III, one of the smallest chromosomes, is forced to missegregate. Plasmid stability decreases when the condensin subunit Brn1 is inactivated. Brn1 is recruited to the plasmid partitioning locus (STB) with the assistance of the plasmid-coded partitioning proteins Rep1 and Rep2. Furthermore, in a dihybrid assay, Brn1 interacts with Rep1-Rep2. Taken together, these findings support a role for condensin and/or condensed chromatin in 2-micron plasmid propagation. They suggest that condensed chromosome loci are among favored sites utilized by the plasmid for its chromosome-associated segregation. By homing to condensed/quiescent chromosome locales, and not over-perturbing genome homeostasis, the plasmid may minimize fitness conflicts with its host. Analogous persistence strategies may be utilized by other extrachromosomal selfish genomes, for example, episomes of mammalian viruses that hitchhike on host chromosomes for their stable maintenance.
format	article
author	Deepanshu Kumar Hemant Kumar Prajapati Anjali Mahilkar Chien-Hui Ma Priyanka Mittal Makkuni Jayaram Santanu K Ghosh
author_facet	Deepanshu Kumar Hemant Kumar Prajapati Anjali Mahilkar Chien-Hui Ma Priyanka Mittal Makkuni Jayaram Santanu K Ghosh
author_sort	Deepanshu Kumar
title	The selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.
title_short	The selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.
title_full	The selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.
title_fullStr	The selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.
title_full_unstemmed	The selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.
title_sort	selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5216b172c1834e6cbed9a58322a9cff2
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The selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.

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