Butanol Purified Food Allergy Herbal Formula-2 Has an Immunomodulating Effect ex-vivo in Pediatric Crohn's Disease Subjects
Background: TNF-α has a major role in the pathogenesis of Crohn's disease (CD). In contrast, GM-CSF may be beneficial for its anti-inflammatory role in a subset of patients with CD with antibodies against GM-CSF as seen in prior trials of GM-CSF which resulted in clinical improvement in CD. We...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:521a66f5222847e49080b9430e771b592021-12-02T01:03:44ZButanol Purified Food Allergy Herbal Formula-2 Has an Immunomodulating Effect ex-vivo in Pediatric Crohn's Disease Subjects2296-858X10.3389/fmed.2021.782859https://doaj.org/article/521a66f5222847e49080b9430e771b592021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmed.2021.782859/fullhttps://doaj.org/toc/2296-858XBackground: TNF-α has a major role in the pathogenesis of Crohn's disease (CD). In contrast, GM-CSF may be beneficial for its anti-inflammatory role in a subset of patients with CD with antibodies against GM-CSF as seen in prior trials of GM-CSF which resulted in clinical improvement in CD. We developed butanol purified Food Allergy Herbal Formula-2 (B-FAHF-2) by refining FAHF-2. FAHF-2 suppressed TNF-α production by human peripheral blood mononuclear cells (PBMCs) and colonic mucosa, and abrogated colitis in a murine model. We sought to examine the effect of B-FAHF-2 and the herbs that comprise it on TNF-α and GM-CSF production as a potential herbal therapy for the treatment of CD.Methods: B-FAHF-2 was examined using high pressure liquid chromatography (HPLC) and compared to the original formulation, FAHF-2. PBMCs from pediatric patients with CD were cultured with lipopolysaccharide and B-FAHF-2, individual herbs or medium alone. Colonic biopsy specimens were cultured with or without B-FAHF-2. TNF-α and GM-CSF were measured by enzyme-linked immunosorbent assay (ELISA). B-FAHF-2 efficacy was tested in vivo in the CD45Rbhi transfer model.Results: B-FAHF-2 had a similar HPLC fingerprint as FAHF-2 but decreased TNF-α production by PBMCs and colonic mucosa from pediatric CD subjects at 20% of the FAHF-2 dose. B-FAHF-2 increased GM-CSF production by PBMCs and colonic mucosa from pediatric CD subjects including those with antibodies to GM-CSF. Of B-FAHF-2's herbal constituents, only Huang Bai suppressed TNF-α and increased GM-CSF production. In the murine model, B-FAHF-2 treatment alleviated colitis.Conclusions: B-FAHF-2 decreased TNF-α production by PBMCs and colonic mucosa from pediatric subjects at a lower dose than FAHF-2. B-FAHF-2 also increased GM-CSF production by PBMCs independent of antibodies. B-FAHF-2 may have a benefit in CD patients.Xin ChenXin ChenJoanne LaiYing SongNan YangNan YangNan YangSacha GnjaticSacha GnjaticVirginia GillespieWilliam HahnEzra ChefitzNanci PittmanJacqueline JossenKeith BenkovMarla DubinskyXiu-Min LiXiu-Min LiDavid DunkinDavid DunkinFrontiers Media S.A.articleCrohn's diseaseherbal therapypre-clinical drug testingimmunomodulatorydrug developmentMedicine (General)R5-920ENFrontiers in Medicine, Vol 8 (2021) |
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| collection |
DOAJ |
| language |
EN |
| topic |
Crohn's disease herbal therapy pre-clinical drug testing immunomodulatory drug development Medicine (General) R5-920 |
| spellingShingle |
Crohn's disease herbal therapy pre-clinical drug testing immunomodulatory drug development Medicine (General) R5-920 Xin Chen Xin Chen Joanne Lai Ying Song Nan Yang Nan Yang Nan Yang Sacha Gnjatic Sacha Gnjatic Virginia Gillespie William Hahn Ezra Chefitz Nanci Pittman Jacqueline Jossen Keith Benkov Marla Dubinsky Xiu-Min Li Xiu-Min Li David Dunkin David Dunkin Butanol Purified Food Allergy Herbal Formula-2 Has an Immunomodulating Effect ex-vivo in Pediatric Crohn's Disease Subjects |
| description |
Background: TNF-α has a major role in the pathogenesis of Crohn's disease (CD). In contrast, GM-CSF may be beneficial for its anti-inflammatory role in a subset of patients with CD with antibodies against GM-CSF as seen in prior trials of GM-CSF which resulted in clinical improvement in CD. We developed butanol purified Food Allergy Herbal Formula-2 (B-FAHF-2) by refining FAHF-2. FAHF-2 suppressed TNF-α production by human peripheral blood mononuclear cells (PBMCs) and colonic mucosa, and abrogated colitis in a murine model. We sought to examine the effect of B-FAHF-2 and the herbs that comprise it on TNF-α and GM-CSF production as a potential herbal therapy for the treatment of CD.Methods: B-FAHF-2 was examined using high pressure liquid chromatography (HPLC) and compared to the original formulation, FAHF-2. PBMCs from pediatric patients with CD were cultured with lipopolysaccharide and B-FAHF-2, individual herbs or medium alone. Colonic biopsy specimens were cultured with or without B-FAHF-2. TNF-α and GM-CSF were measured by enzyme-linked immunosorbent assay (ELISA). B-FAHF-2 efficacy was tested in vivo in the CD45Rbhi transfer model.Results: B-FAHF-2 had a similar HPLC fingerprint as FAHF-2 but decreased TNF-α production by PBMCs and colonic mucosa from pediatric CD subjects at 20% of the FAHF-2 dose. B-FAHF-2 increased GM-CSF production by PBMCs and colonic mucosa from pediatric CD subjects including those with antibodies to GM-CSF. Of B-FAHF-2's herbal constituents, only Huang Bai suppressed TNF-α and increased GM-CSF production. In the murine model, B-FAHF-2 treatment alleviated colitis.Conclusions: B-FAHF-2 decreased TNF-α production by PBMCs and colonic mucosa from pediatric subjects at a lower dose than FAHF-2. B-FAHF-2 also increased GM-CSF production by PBMCs independent of antibodies. B-FAHF-2 may have a benefit in CD patients. |
| format |
article |
| author |
Xin Chen Xin Chen Joanne Lai Ying Song Nan Yang Nan Yang Nan Yang Sacha Gnjatic Sacha Gnjatic Virginia Gillespie William Hahn Ezra Chefitz Nanci Pittman Jacqueline Jossen Keith Benkov Marla Dubinsky Xiu-Min Li Xiu-Min Li David Dunkin David Dunkin |
| author_facet |
Xin Chen Xin Chen Joanne Lai Ying Song Nan Yang Nan Yang Nan Yang Sacha Gnjatic Sacha Gnjatic Virginia Gillespie William Hahn Ezra Chefitz Nanci Pittman Jacqueline Jossen Keith Benkov Marla Dubinsky Xiu-Min Li Xiu-Min Li David Dunkin David Dunkin |
| author_sort |
Xin Chen |
| title |
Butanol Purified Food Allergy Herbal Formula-2 Has an Immunomodulating Effect ex-vivo in Pediatric Crohn's Disease Subjects |
| title_short |
Butanol Purified Food Allergy Herbal Formula-2 Has an Immunomodulating Effect ex-vivo in Pediatric Crohn's Disease Subjects |
| title_full |
Butanol Purified Food Allergy Herbal Formula-2 Has an Immunomodulating Effect ex-vivo in Pediatric Crohn's Disease Subjects |
| title_fullStr |
Butanol Purified Food Allergy Herbal Formula-2 Has an Immunomodulating Effect ex-vivo in Pediatric Crohn's Disease Subjects |
| title_full_unstemmed |
Butanol Purified Food Allergy Herbal Formula-2 Has an Immunomodulating Effect ex-vivo in Pediatric Crohn's Disease Subjects |
| title_sort |
butanol purified food allergy herbal formula-2 has an immunomodulating effect ex-vivo in pediatric crohn's disease subjects |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/521a66f5222847e49080b9430e771b59 |
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