Development, characterization, and applications of multi-material stereolithography bioprinting

Abstract As a 3D bioprinting technique, hydrogel stereolithography has historically been limited in its ability to capture the spatial heterogeneity that permeates mammalian tissues and dictates structure–function relationships. This limitation stems directly from the difficulty of preventing unwant...

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Autores principales:	Bagrat Grigoryan, Daniel W. Sazer, Amanda Avila, Jacob L. Albritton, Aparna Padhye, Anderson H. Ta, Paul T. Greenfield, Don L. Gibbons, Jordan S. Miller
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/52201fe71e8d4cb0922bb80b0d6dcece
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spelling	oai:doaj.org-article:52201fe71e8d4cb0922bb80b0d6dcece2021-12-02T14:06:57ZDevelopment, characterization, and applications of multi-material stereolithography bioprinting10.1038/s41598-021-82102-w2045-2322https://doaj.org/article/52201fe71e8d4cb0922bb80b0d6dcece2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82102-whttps://doaj.org/toc/2045-2322Abstract As a 3D bioprinting technique, hydrogel stereolithography has historically been limited in its ability to capture the spatial heterogeneity that permeates mammalian tissues and dictates structure–function relationships. This limitation stems directly from the difficulty of preventing unwanted material mixing when switching between different liquid bioinks. Accordingly, we present the development, characterization, and application of a multi-material stereolithography bioprinter that provides controlled material selection, yields precise regional feature alignment, and minimizes bioink mixing. Fluorescent tracers were first used to highlight the broad design freedoms afforded by this fabrication strategy, complemented by morphometric image analysis to validate architectural fidelity. To evaluate the bioactivity of printed gels, 344SQ lung adenocarcinoma cells were printed in a 3D core/shell architecture. These cells exhibited native phenotypic behavior as evidenced by apparent proliferation and formation of spherical multicellular aggregates. Cells were also printed as pre-formed multicellular aggregates, which appropriately developed invasive protrusions in response to hTGF-β1. Finally, we constructed a simplified model of intratumoral heterogeneity with two separate sub-populations of 344SQ cells, which together grew over 14 days to form a dense regional interface. Together, these studies highlight the potential of multi-material stereolithography to probe heterotypic interactions between distinct cell types in tissue-specific microenvironments.Bagrat GrigoryanDaniel W. SazerAmanda AvilaJacob L. AlbrittonAparna PadhyeAnderson H. TaPaul T. GreenfieldDon L. GibbonsJordan S. MillerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bagrat Grigoryan Daniel W. Sazer Amanda Avila Jacob L. Albritton Aparna Padhye Anderson H. Ta Paul T. Greenfield Don L. Gibbons Jordan S. Miller Development, characterization, and applications of multi-material stereolithography bioprinting
description	Abstract As a 3D bioprinting technique, hydrogel stereolithography has historically been limited in its ability to capture the spatial heterogeneity that permeates mammalian tissues and dictates structure–function relationships. This limitation stems directly from the difficulty of preventing unwanted material mixing when switching between different liquid bioinks. Accordingly, we present the development, characterization, and application of a multi-material stereolithography bioprinter that provides controlled material selection, yields precise regional feature alignment, and minimizes bioink mixing. Fluorescent tracers were first used to highlight the broad design freedoms afforded by this fabrication strategy, complemented by morphometric image analysis to validate architectural fidelity. To evaluate the bioactivity of printed gels, 344SQ lung adenocarcinoma cells were printed in a 3D core/shell architecture. These cells exhibited native phenotypic behavior as evidenced by apparent proliferation and formation of spherical multicellular aggregates. Cells were also printed as pre-formed multicellular aggregates, which appropriately developed invasive protrusions in response to hTGF-β1. Finally, we constructed a simplified model of intratumoral heterogeneity with two separate sub-populations of 344SQ cells, which together grew over 14 days to form a dense regional interface. Together, these studies highlight the potential of multi-material stereolithography to probe heterotypic interactions between distinct cell types in tissue-specific microenvironments.
format	article
author	Bagrat Grigoryan Daniel W. Sazer Amanda Avila Jacob L. Albritton Aparna Padhye Anderson H. Ta Paul T. Greenfield Don L. Gibbons Jordan S. Miller
author_facet	Bagrat Grigoryan Daniel W. Sazer Amanda Avila Jacob L. Albritton Aparna Padhye Anderson H. Ta Paul T. Greenfield Don L. Gibbons Jordan S. Miller
author_sort	Bagrat Grigoryan
title	Development, characterization, and applications of multi-material stereolithography bioprinting
title_short	Development, characterization, and applications of multi-material stereolithography bioprinting
title_full	Development, characterization, and applications of multi-material stereolithography bioprinting
title_fullStr	Development, characterization, and applications of multi-material stereolithography bioprinting
title_full_unstemmed	Development, characterization, and applications of multi-material stereolithography bioprinting
title_sort	development, characterization, and applications of multi-material stereolithography bioprinting
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/52201fe71e8d4cb0922bb80b0d6dcece
work_keys_str_mv	AT bagratgrigoryan developmentcharacterizationandapplicationsofmultimaterialstereolithographybioprinting AT danielwsazer developmentcharacterizationandapplicationsofmultimaterialstereolithographybioprinting AT amandaavila developmentcharacterizationandapplicationsofmultimaterialstereolithographybioprinting AT jacoblalbritton developmentcharacterizationandapplicationsofmultimaterialstereolithographybioprinting AT aparnapadhye developmentcharacterizationandapplicationsofmultimaterialstereolithographybioprinting AT andersonhta developmentcharacterizationandapplicationsofmultimaterialstereolithographybioprinting AT paultgreenfield developmentcharacterizationandapplicationsofmultimaterialstereolithographybioprinting AT donlgibbons developmentcharacterizationandapplicationsofmultimaterialstereolithographybioprinting AT jordansmiller developmentcharacterizationandapplicationsofmultimaterialstereolithographybioprinting
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Development, characterization, and applications of multi-material stereolithography bioprinting

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