Electrospun Amphiphilic Nanofibers as Templates for In Situ Preparation of Chloramphenicol-Loaded Liposomes

The hydration of phospholipids, electrospun into polymeric nanofibers and used as templates for liposome formation, offers pharmaceutical advantages as it avoids the storage of liposomes as aqueous dispersions. The objective of the present study was to electrospin and characterize amphiphilic nanofi...

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Autores principales:	Ivo Laidmäe, Andres Meos, Irja Alainezhad Kjærvik, Sveinung G. Ingebrigtsen, Nataša Škalko-Basnet, Kalle Kirsimäe, Tavo Romann, Urmas Joost, Vambola Kisand, Karin Kogermann
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	liposome electrospinning amphiphilic nanofibers film hydration drug release chloramphenicol Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/52294bd6303140489a4eda5d727cdff5
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spelling	oai:doaj.org-article:52294bd6303140489a4eda5d727cdff52021-11-25T18:40:18ZElectrospun Amphiphilic Nanofibers as Templates for In Situ Preparation of Chloramphenicol-Loaded Liposomes10.3390/pharmaceutics131117421999-4923https://doaj.org/article/52294bd6303140489a4eda5d727cdff52021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1742https://doaj.org/toc/1999-4923The hydration of phospholipids, electrospun into polymeric nanofibers and used as templates for liposome formation, offers pharmaceutical advantages as it avoids the storage of liposomes as aqueous dispersions. The objective of the present study was to electrospin and characterize amphiphilic nanofibers as templates for the preparation of antibiotic-loaded liposomes and compare this method with the conventional film-hydration method followed by extrusion. The comparison was based on particle size, encapsulation efficiency and drug-release behavior. Chloramphenicol (CAM) was used at different concentrations as a model antibacterial drug. Phosphatidylcoline (PC) with polyvinylpyrrolidone (PVP), using ethanol as a solvent, was found to be successful in fabricating the amphiphilic composite drug-loaded nanofibers as well as liposomes with both methods. The characterization of the nanofiber templates revealed that fiber diameter did not affect the liposome size. According to the optical microscopy results, the immediate hydration of phospholipids deposited on the amphiphilic nanofibers occurred within a few seconds, resulting in the formation of liposomes in water dispersions. The liposomes appeared to aggregate more readily in the concentrated than in the diluted solutions. The drug encapsulation efficiency for the fiber-hydrated liposomes varied between 14.9 and 28.1% and, for film-hydrated liposomes, between 22.0 and 77.1%, depending on the CAM concentrations and additional extrusion steps. The nanofiber hydration method was faster, as less steps were required for the in-situ liposome preparation than in the film-hydration method. The liposomes obtained using nanofiber hydration were smaller and more homogeneous than the conventional liposomes, but less drug was encapsulated.Ivo LaidmäeAndres MeosIrja Alainezhad KjærvikSveinung G. IngebrigtsenNataša Škalko-BasnetKalle KirsimäeTavo RomannUrmas JoostVambola KisandKarin KogermannMDPI AGarticleliposomeelectrospinningamphiphilic nanofibersfilm hydrationdrug releasechloramphenicolPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1742, p 1742 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	liposome electrospinning amphiphilic nanofibers film hydration drug release chloramphenicol Pharmacy and materia medica RS1-441
spellingShingle	liposome electrospinning amphiphilic nanofibers film hydration drug release chloramphenicol Pharmacy and materia medica RS1-441 Ivo Laidmäe Andres Meos Irja Alainezhad Kjærvik Sveinung G. Ingebrigtsen Nataša Škalko-Basnet Kalle Kirsimäe Tavo Romann Urmas Joost Vambola Kisand Karin Kogermann Electrospun Amphiphilic Nanofibers as Templates for In Situ Preparation of Chloramphenicol-Loaded Liposomes
description	The hydration of phospholipids, electrospun into polymeric nanofibers and used as templates for liposome formation, offers pharmaceutical advantages as it avoids the storage of liposomes as aqueous dispersions. The objective of the present study was to electrospin and characterize amphiphilic nanofibers as templates for the preparation of antibiotic-loaded liposomes and compare this method with the conventional film-hydration method followed by extrusion. The comparison was based on particle size, encapsulation efficiency and drug-release behavior. Chloramphenicol (CAM) was used at different concentrations as a model antibacterial drug. Phosphatidylcoline (PC) with polyvinylpyrrolidone (PVP), using ethanol as a solvent, was found to be successful in fabricating the amphiphilic composite drug-loaded nanofibers as well as liposomes with both methods. The characterization of the nanofiber templates revealed that fiber diameter did not affect the liposome size. According to the optical microscopy results, the immediate hydration of phospholipids deposited on the amphiphilic nanofibers occurred within a few seconds, resulting in the formation of liposomes in water dispersions. The liposomes appeared to aggregate more readily in the concentrated than in the diluted solutions. The drug encapsulation efficiency for the fiber-hydrated liposomes varied between 14.9 and 28.1% and, for film-hydrated liposomes, between 22.0 and 77.1%, depending on the CAM concentrations and additional extrusion steps. The nanofiber hydration method was faster, as less steps were required for the in-situ liposome preparation than in the film-hydration method. The liposomes obtained using nanofiber hydration were smaller and more homogeneous than the conventional liposomes, but less drug was encapsulated.
format	article
author	Ivo Laidmäe Andres Meos Irja Alainezhad Kjærvik Sveinung G. Ingebrigtsen Nataša Škalko-Basnet Kalle Kirsimäe Tavo Romann Urmas Joost Vambola Kisand Karin Kogermann
author_facet	Ivo Laidmäe Andres Meos Irja Alainezhad Kjærvik Sveinung G. Ingebrigtsen Nataša Škalko-Basnet Kalle Kirsimäe Tavo Romann Urmas Joost Vambola Kisand Karin Kogermann
author_sort	Ivo Laidmäe
title	Electrospun Amphiphilic Nanofibers as Templates for In Situ Preparation of Chloramphenicol-Loaded Liposomes
title_short	Electrospun Amphiphilic Nanofibers as Templates for In Situ Preparation of Chloramphenicol-Loaded Liposomes
title_full	Electrospun Amphiphilic Nanofibers as Templates for In Situ Preparation of Chloramphenicol-Loaded Liposomes
title_fullStr	Electrospun Amphiphilic Nanofibers as Templates for In Situ Preparation of Chloramphenicol-Loaded Liposomes
title_full_unstemmed	Electrospun Amphiphilic Nanofibers as Templates for In Situ Preparation of Chloramphenicol-Loaded Liposomes
title_sort	electrospun amphiphilic nanofibers as templates for in situ preparation of chloramphenicol-loaded liposomes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/52294bd6303140489a4eda5d727cdff5
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Electrospun Amphiphilic Nanofibers as Templates for In Situ Preparation of Chloramphenicol-Loaded Liposomes

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