The β-hemolysin and intracellular survival of Streptococcus agalactiae in human macrophages.

S. agalactiae (group B streptococci, GBS) is a major microbial pathogen in human neonates and causes invasive infections in pregnant women and immunocompromised individuals. The S. agalactiae β-hemolysin is regarded as an important virulence factor for the development of invasive disease. To examine...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anubha Sagar, Carolin Klemm, Lara Hartjes, Stefanie Mauerer, Ger van Zandbergen, Barbara Spellerberg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/522c63ff856b4d928d25722ee3d977d6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:522c63ff856b4d928d25722ee3d977d6
record_format	dspace
spelling	oai:doaj.org-article:522c63ff856b4d928d25722ee3d977d62021-11-18T07:50:39ZThe β-hemolysin and intracellular survival of Streptococcus agalactiae in human macrophages.1932-620310.1371/journal.pone.0060160https://doaj.org/article/522c63ff856b4d928d25722ee3d977d62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23593170/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203S. agalactiae (group B streptococci, GBS) is a major microbial pathogen in human neonates and causes invasive infections in pregnant women and immunocompromised individuals. The S. agalactiae β-hemolysin is regarded as an important virulence factor for the development of invasive disease. To examine the role of β-hemolysin in the interaction with professional phagocytes, the THP-1 monocytic cell line and human granulocytes were infected with a serotype Ia S. agalactiae wild type strain and its isogenic nonhemolytic mutant. We could show that the nonhemolytic mutants were able to survive in significantly higher numbers than the hemolytic wild type strain, in THP-1 macrophage-like cells and in assays with human granulocytes. Intracellular bacterial multiplication, however, could not be observed. The hemolytic wild type strain stimulated a significantly higher release of Tumor Necrosis Factor-α than the nonhemolytic mutant in THP-1 cells, while similar levels of the chemokine Interleukin-8 were induced. In order to investigate bacterial mediators of IL-8 release in this setting, purified cell wall preparations from both strains were tested and found to exert a potent proinflammatory stimulus on THP-1 cells. In conclusion, our results indicate that the β-hemolysin has a strong influence on the intracellular survival of S. agalactiae and that a tightly controlled regulation of β-hemolysin expression is required for the successful establishment of S. agalactiae in different host niches.Anubha SagarCarolin KlemmLara HartjesStefanie MauererGer van ZandbergenBarbara SpellerbergPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e60160 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anubha Sagar Carolin Klemm Lara Hartjes Stefanie Mauerer Ger van Zandbergen Barbara Spellerberg The β-hemolysin and intracellular survival of Streptococcus agalactiae in human macrophages.
description	S. agalactiae (group B streptococci, GBS) is a major microbial pathogen in human neonates and causes invasive infections in pregnant women and immunocompromised individuals. The S. agalactiae β-hemolysin is regarded as an important virulence factor for the development of invasive disease. To examine the role of β-hemolysin in the interaction with professional phagocytes, the THP-1 monocytic cell line and human granulocytes were infected with a serotype Ia S. agalactiae wild type strain and its isogenic nonhemolytic mutant. We could show that the nonhemolytic mutants were able to survive in significantly higher numbers than the hemolytic wild type strain, in THP-1 macrophage-like cells and in assays with human granulocytes. Intracellular bacterial multiplication, however, could not be observed. The hemolytic wild type strain stimulated a significantly higher release of Tumor Necrosis Factor-α than the nonhemolytic mutant in THP-1 cells, while similar levels of the chemokine Interleukin-8 were induced. In order to investigate bacterial mediators of IL-8 release in this setting, purified cell wall preparations from both strains were tested and found to exert a potent proinflammatory stimulus on THP-1 cells. In conclusion, our results indicate that the β-hemolysin has a strong influence on the intracellular survival of S. agalactiae and that a tightly controlled regulation of β-hemolysin expression is required for the successful establishment of S. agalactiae in different host niches.
format	article
author	Anubha Sagar Carolin Klemm Lara Hartjes Stefanie Mauerer Ger van Zandbergen Barbara Spellerberg
author_facet	Anubha Sagar Carolin Klemm Lara Hartjes Stefanie Mauerer Ger van Zandbergen Barbara Spellerberg
author_sort	Anubha Sagar
title	The β-hemolysin and intracellular survival of Streptococcus agalactiae in human macrophages.
title_short	The β-hemolysin and intracellular survival of Streptococcus agalactiae in human macrophages.
title_full	The β-hemolysin and intracellular survival of Streptococcus agalactiae in human macrophages.
title_fullStr	The β-hemolysin and intracellular survival of Streptococcus agalactiae in human macrophages.
title_full_unstemmed	The β-hemolysin and intracellular survival of Streptococcus agalactiae in human macrophages.
title_sort	β-hemolysin and intracellular survival of streptococcus agalactiae in human macrophages.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/522c63ff856b4d928d25722ee3d977d6
work_keys_str_mv	AT anubhasagar thebhemolysinandintracellularsurvivalofstreptococcusagalactiaeinhumanmacrophages AT carolinklemm thebhemolysinandintracellularsurvivalofstreptococcusagalactiaeinhumanmacrophages AT larahartjes thebhemolysinandintracellularsurvivalofstreptococcusagalactiaeinhumanmacrophages AT stefaniemauerer thebhemolysinandintracellularsurvivalofstreptococcusagalactiaeinhumanmacrophages AT gervanzandbergen thebhemolysinandintracellularsurvivalofstreptococcusagalactiaeinhumanmacrophages AT barbaraspellerberg thebhemolysinandintracellularsurvivalofstreptococcusagalactiaeinhumanmacrophages AT anubhasagar bhemolysinandintracellularsurvivalofstreptococcusagalactiaeinhumanmacrophages AT carolinklemm bhemolysinandintracellularsurvivalofstreptococcusagalactiaeinhumanmacrophages AT larahartjes bhemolysinandintracellularsurvivalofstreptococcusagalactiaeinhumanmacrophages AT stefaniemauerer bhemolysinandintracellularsurvivalofstreptococcusagalactiaeinhumanmacrophages AT gervanzandbergen bhemolysinandintracellularsurvivalofstreptococcusagalactiaeinhumanmacrophages AT barbaraspellerberg bhemolysinandintracellularsurvivalofstreptococcusagalactiaeinhumanmacrophages
_version_	1718422851227222016

The β-hemolysin and intracellular survival of Streptococcus agalactiae in human macrophages.

Ejemplares similares