Synthesis and in vivo magnetic resonance imaging evaluation of biocompatible branched copolymer nanocontrast agents

Alexander W Jackson,1,* Prashant Chandrasekharan,2,* Jian Shi,3 Steven P Rannard,4 Quan Liu,5 Chang-Tong Yang,6 Tao He1,7 1Institute of Chemical and Engineering Sciences (ICES), 2Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*&nbs...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jackson AW, Chandrasekharan P, Shi J, Rannard SP, Liu Q, Yang CT, He T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5239389086fe41d7b7ee03f1435237db
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5239389086fe41d7b7ee03f1435237db
record_format	dspace
spelling	oai:doaj.org-article:5239389086fe41d7b7ee03f1435237db2021-12-02T03:11:42ZSynthesis and in vivo magnetic resonance imaging evaluation of biocompatible branched copolymer nanocontrast agents1178-2013https://doaj.org/article/5239389086fe41d7b7ee03f1435237db2015-09-01T00:00:00Zhttps://www.dovepress.com/synthesis-and-in-vivo-magnetic-resonance-imaging-evaluation-of-biocomp-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Alexander W Jackson,1,* Prashant Chandrasekharan,2,* Jian Shi,3 Steven P Rannard,4 Quan Liu,5 Chang-Tong Yang,6 Tao He1,7 1Institute of Chemical and Engineering Sciences (ICES), 2Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A* STAR), 3Department of Biological Science, National University of Singapore, Singapore; 4Department of Chemistry, University of Liverpool, Liverpool, United Kingdom; 5School of Chemical and Biomedical Engineering, 6Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore; 7School of Chemistry and Chemical Engineering, HeFei University of Technology, Anhui, People’s Republic of China *These authors contributed equally to this work Abstract: Branched copolymer nanoparticles (Dh =20–35 nm) possessing 1,4,7, 10-tetraazacyclododecane-N,N',N",N'"-tetraacetic acid macrocycles within their cores have been synthesized and applied as magnetic resonance imaging (MRI) nanosized contrast agents in vivo. These nanoparticles have been generated from novel functional monomers via reversible addition–fragmentation chain transfer polymerization. The process is very robust and synthetically straightforward. Chelation with gadolinium and preliminary in vivo experiments have demonstrated promising characteristics as MRI contrast agents with prolonged blood retention time, good biocompatibility, and an intravascular distribution. The ability of these nanoparticles to perfuse and passively target tumor cells through the enhanced permeability and retention effect is also demonstrated. These novel highly functional nanoparticle platforms have succinimidyl ester-activated benzoate functionalities within their corona, which make them suitable for future peptide conjugation and subsequent active cell-targeted MRI or the conjugation of fluorophores for bimodal imaging. We have also demonstrated that these branched copolymer nanoparticles are able to noncovalently encapsulate hydrophobic guest molecules, which could allow simultaneous bioimaging and drug delivery. Keywords: branched copolymer nanoparticles, gadolinium chelate, MRI, RAFT polymerizationJackson AWChandrasekharan PShi JRannard SPLiu QYang CTHe TDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 5895-5907 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Jackson AW Chandrasekharan P Shi J Rannard SP Liu Q Yang CT He T Synthesis and in vivo magnetic resonance imaging evaluation of biocompatible branched copolymer nanocontrast agents
description	Alexander W Jackson,1,* Prashant Chandrasekharan,2,* Jian Shi,3 Steven P Rannard,4 Quan Liu,5 Chang-Tong Yang,6 Tao He1,7 1Institute of Chemical and Engineering Sciences (ICES), 2Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A* STAR), 3Department of Biological Science, National University of Singapore, Singapore; 4Department of Chemistry, University of Liverpool, Liverpool, United Kingdom; 5School of Chemical and Biomedical Engineering, 6Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore; 7School of Chemistry and Chemical Engineering, HeFei University of Technology, Anhui, People’s Republic of China *These authors contributed equally to this work Abstract: Branched copolymer nanoparticles (Dh =20–35 nm) possessing 1,4,7, 10-tetraazacyclododecane-N,N',N",N'"-tetraacetic acid macrocycles within their cores have been synthesized and applied as magnetic resonance imaging (MRI) nanosized contrast agents in vivo. These nanoparticles have been generated from novel functional monomers via reversible addition–fragmentation chain transfer polymerization. The process is very robust and synthetically straightforward. Chelation with gadolinium and preliminary in vivo experiments have demonstrated promising characteristics as MRI contrast agents with prolonged blood retention time, good biocompatibility, and an intravascular distribution. The ability of these nanoparticles to perfuse and passively target tumor cells through the enhanced permeability and retention effect is also demonstrated. These novel highly functional nanoparticle platforms have succinimidyl ester-activated benzoate functionalities within their corona, which make them suitable for future peptide conjugation and subsequent active cell-targeted MRI or the conjugation of fluorophores for bimodal imaging. We have also demonstrated that these branched copolymer nanoparticles are able to noncovalently encapsulate hydrophobic guest molecules, which could allow simultaneous bioimaging and drug delivery. Keywords: branched copolymer nanoparticles, gadolinium chelate, MRI, RAFT polymerization
format	article
author	Jackson AW Chandrasekharan P Shi J Rannard SP Liu Q Yang CT He T
author_facet	Jackson AW Chandrasekharan P Shi J Rannard SP Liu Q Yang CT He T
author_sort	Jackson AW
title	Synthesis and in vivo magnetic resonance imaging evaluation of biocompatible branched copolymer nanocontrast agents
title_short	Synthesis and in vivo magnetic resonance imaging evaluation of biocompatible branched copolymer nanocontrast agents
title_full	Synthesis and in vivo magnetic resonance imaging evaluation of biocompatible branched copolymer nanocontrast agents
title_fullStr	Synthesis and in vivo magnetic resonance imaging evaluation of biocompatible branched copolymer nanocontrast agents
title_full_unstemmed	Synthesis and in vivo magnetic resonance imaging evaluation of biocompatible branched copolymer nanocontrast agents
title_sort	synthesis and in vivo magnetic resonance imaging evaluation of biocompatible branched copolymer nanocontrast agents
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/5239389086fe41d7b7ee03f1435237db
work_keys_str_mv	AT jacksonaw synthesisandinvivomagneticresonanceimagingevaluationofbiocompatiblebranchedcopolymernanocontrastagents AT chandrasekharanp synthesisandinvivomagneticresonanceimagingevaluationofbiocompatiblebranchedcopolymernanocontrastagents AT shij synthesisandinvivomagneticresonanceimagingevaluationofbiocompatiblebranchedcopolymernanocontrastagents AT rannardsp synthesisandinvivomagneticresonanceimagingevaluationofbiocompatiblebranchedcopolymernanocontrastagents AT liuq synthesisandinvivomagneticresonanceimagingevaluationofbiocompatiblebranchedcopolymernanocontrastagents AT yangct synthesisandinvivomagneticresonanceimagingevaluationofbiocompatiblebranchedcopolymernanocontrastagents AT het synthesisandinvivomagneticresonanceimagingevaluationofbiocompatiblebranchedcopolymernanocontrastagents
_version_	1718401852828024832

Synthesis and in vivo magnetic resonance imaging evaluation of biocompatible branched copolymer nanocontrast agents

Ejemplares similares