Ex vivo laser confocal microscopy findings of cultured Acanthamoeba trophozoites

Natsuko Yamazaki,1 Akira Kobayashi,1 Hideaki Yokogawa,1 Yasuhisa Ishibashi,2 Yosaburo Oikawa,3 Masaharu Tokoro,4 Kazuhisa Sugiyama11Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan; 2Department of Ophthalmology, East Washinomiya Hospital, Kuki, Jap...

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Autores principales:	Yamazaki N, Kobayashi A, Yokogawa H, Ishibashi Y, Oikawa Y, Tokoro M, Sugiyama K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/523b2d177ecc4306810f851aa896bfb2
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spelling	oai:doaj.org-article:523b2d177ecc4306810f851aa896bfb22021-12-02T05:09:24ZEx vivo laser confocal microscopy findings of cultured Acanthamoeba trophozoites1177-54671177-5483https://doaj.org/article/523b2d177ecc4306810f851aa896bfb22012-08-01T00:00:00Zhttp://www.dovepress.com/ex-vivo-laser-confocal-microscopy-findings-of-cultured-acanthamoeba-tr-a10771https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Natsuko Yamazaki,1 Akira Kobayashi,1 Hideaki Yokogawa,1 Yasuhisa Ishibashi,2 Yosaburo Oikawa,3 Masaharu Tokoro,4 Kazuhisa Sugiyama11Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan; 2Department of Ophthalmology, East Washinomiya Hospital, Kuki, Japan; 3Department of Medical Zoology, Kanazawa Medical University, Kahoku, Japan; 4Department of Parasitology, Kanazawa University Graduate School of Medical Science, Kanazawa, JapanPurpose: The purpose of the current study was to investigate ex vivo laser confocal microscopic findings of cultured Acanthamoeba trophozoites obtained from Acanthamoeba keratitis patients.Methods: Eight cultured samples of Acanthamoeba trophozoites from eight eyes of seven patients (mean age, 26.9 years; age range, 18&ndash;52 years) were used. Seven samples were from corneal scrapings of Acanthamoeba keratitis patients and one sample was from the solution in a soft contact lens case. Ex vivo laser confocal microscopy was performed to qualitatively evaluate the shape and degree of light reflection of the living Acanthamoeba trophozoites.Results: Ex vivo laser confocal microscopy demonstrated highly reflective, high-contrast Acanthamoeba trophozoites with no walls (mean size, 25.4 &micro;m; range, 17.1&ndash;58.5 &micro;m). The shapes of the trophozoites were highly pleomorphic, and some showed characteristic acanthopodia by laser confocal microscopy.Conclusion: Ex vivo laser confocal microscopy was effective in demonstrating cultured Acanthamoeba trophozoites of various shapes and sizes. The observations of the current study may be helpful when similar structures are identified under in vivo conditions.Keywords: Acanthamoeba, trophozoite, laser confocal microscopyYamazaki NKobayashi AYokogawa HIshibashi YOikawa YTokoro MSugiyama KDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2012, Iss default, Pp 1365-1368 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Yamazaki N Kobayashi A Yokogawa H Ishibashi Y Oikawa Y Tokoro M Sugiyama K Ex vivo laser confocal microscopy findings of cultured Acanthamoeba trophozoites
description	Natsuko Yamazaki,1 Akira Kobayashi,1 Hideaki Yokogawa,1 Yasuhisa Ishibashi,2 Yosaburo Oikawa,3 Masaharu Tokoro,4 Kazuhisa Sugiyama11Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan; 2Department of Ophthalmology, East Washinomiya Hospital, Kuki, Japan; 3Department of Medical Zoology, Kanazawa Medical University, Kahoku, Japan; 4Department of Parasitology, Kanazawa University Graduate School of Medical Science, Kanazawa, JapanPurpose: The purpose of the current study was to investigate ex vivo laser confocal microscopic findings of cultured Acanthamoeba trophozoites obtained from Acanthamoeba keratitis patients.Methods: Eight cultured samples of Acanthamoeba trophozoites from eight eyes of seven patients (mean age, 26.9 years; age range, 18&ndash;52 years) were used. Seven samples were from corneal scrapings of Acanthamoeba keratitis patients and one sample was from the solution in a soft contact lens case. Ex vivo laser confocal microscopy was performed to qualitatively evaluate the shape and degree of light reflection of the living Acanthamoeba trophozoites.Results: Ex vivo laser confocal microscopy demonstrated highly reflective, high-contrast Acanthamoeba trophozoites with no walls (mean size, 25.4 &micro;m; range, 17.1&ndash;58.5 &micro;m). The shapes of the trophozoites were highly pleomorphic, and some showed characteristic acanthopodia by laser confocal microscopy.Conclusion: Ex vivo laser confocal microscopy was effective in demonstrating cultured Acanthamoeba trophozoites of various shapes and sizes. The observations of the current study may be helpful when similar structures are identified under in vivo conditions.Keywords: Acanthamoeba, trophozoite, laser confocal microscopy
format	article
author	Yamazaki N Kobayashi A Yokogawa H Ishibashi Y Oikawa Y Tokoro M Sugiyama K
author_facet	Yamazaki N Kobayashi A Yokogawa H Ishibashi Y Oikawa Y Tokoro M Sugiyama K
author_sort	Yamazaki N
title	Ex vivo laser confocal microscopy findings of cultured Acanthamoeba trophozoites
title_short	Ex vivo laser confocal microscopy findings of cultured Acanthamoeba trophozoites
title_full	Ex vivo laser confocal microscopy findings of cultured Acanthamoeba trophozoites
title_fullStr	Ex vivo laser confocal microscopy findings of cultured Acanthamoeba trophozoites
title_full_unstemmed	Ex vivo laser confocal microscopy findings of cultured Acanthamoeba trophozoites
title_sort	ex vivo laser confocal microscopy findings of cultured acanthamoeba trophozoites
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/523b2d177ecc4306810f851aa896bfb2
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Ex vivo laser confocal microscopy findings of cultured Acanthamoeba trophozoites

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