Transcriptome Analysis of Testicular Aging in Mice

Male reproductive aging, or andropause, is associated with gradual age-related changes in testicular properties, sperm production, and erectile function. The testis, which is the primary male reproductive organ, produces sperm and androgens. To understand the transcriptional changes underlying male...

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Autores principales:	Gwidong Han, Seong-Hyeon Hong, Seung-Jae Lee, Seung-Pyo Hong, Chunghee Cho
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	spermatogenesis testis aging long non-coding RNA testicular aging andropause Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/523c36c867234e649d0e8f6e41f92191
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spelling	oai:doaj.org-article:523c36c867234e649d0e8f6e41f921912021-11-25T17:08:35ZTranscriptome Analysis of Testicular Aging in Mice10.3390/cells101128952073-4409https://doaj.org/article/523c36c867234e649d0e8f6e41f921912021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2895https://doaj.org/toc/2073-4409Male reproductive aging, or andropause, is associated with gradual age-related changes in testicular properties, sperm production, and erectile function. The testis, which is the primary male reproductive organ, produces sperm and androgens. To understand the transcriptional changes underlying male reproductive aging, we performed transcriptome analysis of aging testes in mice. A total of 31,386 mRNAs and 9387 long non-coding RNAs (lncRNAs) were identified in the mouse testes of diverse age groups (3, 6, 12, and 18 months old) by total RNA sequencing. Of them, 1571 mRNAs and 715 lncRNAs exhibited changes in their levels during testicular aging. Most of these aging-related transcripts exhibited slight and continuous expression changes during aging, whereas some (9.6%) showed larger expression changes. The aging-related transcripts could be classified into diverse expression patterns, in which the transcripts changed mainly at 3–6 months or at 12–18 months. Our subsequent in silico analysis provided insight into the potential features of testicular aging-related mRNAs and lncRNAs. We identified testis-specific aging-related transcripts (121 mRNAs and 25 lncRNAs) by comparison with a known testis-specific transcript profile, and then predicted the potential reproduction-related functions of the mRNAs. By selecting transcripts that are altered only between 3 and 18 months, we identified 46 mRNAs and 34 lncRNAs that are stringently related to the terminal stage of male reproductive aging. Some of these mRNAs were related to hormonal regulation. Finally, our in silico analysis of the 34 aging-related lncRNAs revealed that they co-localized with 19 testis-expressed protein-coding genes, 13 of which are considered to show testis-specific or -predominant expression. These nearby genes could be potential targets of <i>cis</i>-regulation by the aging-related lncRNAs. Collectively, our results identify a number of testicular aging-related mRNAs and lncRNAs in mice and provide a basis for the future investigation of these transcripts in the context of aging-associated testicular dysfunction.Gwidong HanSeong-Hyeon HongSeung-Jae LeeSeung-Pyo HongChunghee ChoMDPI AGarticlespermatogenesistestisaginglong non-coding RNAtesticular agingandropauseBiology (General)QH301-705.5ENCells, Vol 10, Iss 2895, p 2895 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	spermatogenesis testis aging long non-coding RNA testicular aging andropause Biology (General) QH301-705.5
spellingShingle	spermatogenesis testis aging long non-coding RNA testicular aging andropause Biology (General) QH301-705.5 Gwidong Han Seong-Hyeon Hong Seung-Jae Lee Seung-Pyo Hong Chunghee Cho Transcriptome Analysis of Testicular Aging in Mice
description	Male reproductive aging, or andropause, is associated with gradual age-related changes in testicular properties, sperm production, and erectile function. The testis, which is the primary male reproductive organ, produces sperm and androgens. To understand the transcriptional changes underlying male reproductive aging, we performed transcriptome analysis of aging testes in mice. A total of 31,386 mRNAs and 9387 long non-coding RNAs (lncRNAs) were identified in the mouse testes of diverse age groups (3, 6, 12, and 18 months old) by total RNA sequencing. Of them, 1571 mRNAs and 715 lncRNAs exhibited changes in their levels during testicular aging. Most of these aging-related transcripts exhibited slight and continuous expression changes during aging, whereas some (9.6%) showed larger expression changes. The aging-related transcripts could be classified into diverse expression patterns, in which the transcripts changed mainly at 3–6 months or at 12–18 months. Our subsequent in silico analysis provided insight into the potential features of testicular aging-related mRNAs and lncRNAs. We identified testis-specific aging-related transcripts (121 mRNAs and 25 lncRNAs) by comparison with a known testis-specific transcript profile, and then predicted the potential reproduction-related functions of the mRNAs. By selecting transcripts that are altered only between 3 and 18 months, we identified 46 mRNAs and 34 lncRNAs that are stringently related to the terminal stage of male reproductive aging. Some of these mRNAs were related to hormonal regulation. Finally, our in silico analysis of the 34 aging-related lncRNAs revealed that they co-localized with 19 testis-expressed protein-coding genes, 13 of which are considered to show testis-specific or -predominant expression. These nearby genes could be potential targets of <i>cis</i>-regulation by the aging-related lncRNAs. Collectively, our results identify a number of testicular aging-related mRNAs and lncRNAs in mice and provide a basis for the future investigation of these transcripts in the context of aging-associated testicular dysfunction.
format	article
author	Gwidong Han Seong-Hyeon Hong Seung-Jae Lee Seung-Pyo Hong Chunghee Cho
author_facet	Gwidong Han Seong-Hyeon Hong Seung-Jae Lee Seung-Pyo Hong Chunghee Cho
author_sort	Gwidong Han
title	Transcriptome Analysis of Testicular Aging in Mice
title_short	Transcriptome Analysis of Testicular Aging in Mice
title_full	Transcriptome Analysis of Testicular Aging in Mice
title_fullStr	Transcriptome Analysis of Testicular Aging in Mice
title_full_unstemmed	Transcriptome Analysis of Testicular Aging in Mice
title_sort	transcriptome analysis of testicular aging in mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/523c36c867234e649d0e8f6e41f92191
work_keys_str_mv	AT gwidonghan transcriptomeanalysisoftesticularaginginmice AT seonghyeonhong transcriptomeanalysisoftesticularaginginmice AT seungjaelee transcriptomeanalysisoftesticularaginginmice AT seungpyohong transcriptomeanalysisoftesticularaginginmice AT chungheecho transcriptomeanalysisoftesticularaginginmice
_version_	1718412648510390272

Transcriptome Analysis of Testicular Aging in Mice

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