FEATURES OF LOCAL mRNA SYNTHESIS FOR SOME CC- AND CXC-CHEMOKINES AND THEIR RECEPTORS IN ENDOMETRIAL HYPERPLASIA

Аbstract. Endometrial hyperplasia (EH) represents an excessive increase in thickness and volume of proliferating endometrium accompanied by altered glandular structure. This disorder is higly prevalent among women in their premenopausal period. There exist only scarce data concerning pos...

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Autores principales:	N. V. Kipich, N. V. Kulagina, A. B. Chukhlovin, K. A. Syssoev, Areg A. Totolian
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	endometrial hyperplasia chemokines chemokine receptors mrna synthesis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/523cff2a577249c197b272c7f14e9035
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spelling	oai:doaj.org-article:523cff2a577249c197b272c7f14e90352021-11-18T08:03:42ZFEATURES OF LOCAL mRNA SYNTHESIS FOR SOME CC- AND CXC-CHEMOKINES AND THEIR RECEPTORS IN ENDOMETRIAL HYPERPLASIA1563-06252313-741X10.15789/1563-0625-2011-2-3-189-196https://doaj.org/article/523cff2a577249c197b272c7f14e90352014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/483https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XАbstract. Endometrial hyperplasia (EH) represents an excessive increase in thickness and volume of proliferating endometrium accompanied by altered glandular structure. This disorder is higly prevalent among women in their premenopausal period. There exist only scarce data concerning possible role of chemokines and their receptors in EH pathogenesis and clinical course. Hence, the aim of our study was to analyze mRNA expression of several key chemokines and their receptors in endometrial tissue samples from EH patients. This work included sixty-three women with disturbed menstrual cycle and/or pathological changes of endometrium, as assessed by sonographic studies. The patients were 32 to 61 years old (a mean of 48.4±0.6 years). The levels of mRNA expression were determined by gene-specific PCR in a semiquantitative manner, whereas promoter genotypes of matrix metalloproteinases (ММР1 -16071G/2G and ММР3 -11715А/6A) were identified by means of allele-specific PCR. Results of the study included a significant increase of mRNA for MIP-1α, eotaxin 2, along with decreased amounts of mRNA for CCR-3 (a specific receptor for eotaxins), in polyps developing from hyperplastic endometrium. MIP-1α synthesis fades away with increasing age. An increased level of MIP-1β was shown in prolonged and recurrent disturbances of menstrual cycle, whereas elevation of MIP-1α and CXCR-1 was registered in cases of multiple pregnancies. In threatening abortions, an increase of MIP-1β gene expression was revealed. Hence, the local chemokine system reacts to inflammatory and hemorrhagic complications with increased mRNA expression of certain chemokine genes. Determination of the chemokine mRNA levels, as well as their receptors in patients with endometrial hyperplasia may reflect a general background of this disorder. (Med. Immunol., 2011, vol. 13, N 2-3, pp 189-196)N. V. KipichN. V. KulaginaA. B. ChukhlovinK. A. SyssoevAreg A. TotolianSPb RAACIarticleendometrial hyperplasiachemokineschemokine receptorsmrna synthesisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 13, Iss 2-3, Pp 189-196 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	endometrial hyperplasia chemokines chemokine receptors mrna synthesis Immunologic diseases. Allergy RC581-607
spellingShingle	endometrial hyperplasia chemokines chemokine receptors mrna synthesis Immunologic diseases. Allergy RC581-607 N. V. Kipich N. V. Kulagina A. B. Chukhlovin K. A. Syssoev Areg A. Totolian FEATURES OF LOCAL mRNA SYNTHESIS FOR SOME CC- AND CXC-CHEMOKINES AND THEIR RECEPTORS IN ENDOMETRIAL HYPERPLASIA
description	Аbstract. Endometrial hyperplasia (EH) represents an excessive increase in thickness and volume of proliferating endometrium accompanied by altered glandular structure. This disorder is higly prevalent among women in their premenopausal period. There exist only scarce data concerning possible role of chemokines and their receptors in EH pathogenesis and clinical course. Hence, the aim of our study was to analyze mRNA expression of several key chemokines and their receptors in endometrial tissue samples from EH patients. This work included sixty-three women with disturbed menstrual cycle and/or pathological changes of endometrium, as assessed by sonographic studies. The patients were 32 to 61 years old (a mean of 48.4±0.6 years). The levels of mRNA expression were determined by gene-specific PCR in a semiquantitative manner, whereas promoter genotypes of matrix metalloproteinases (ММР1 -16071G/2G and ММР3 -11715А/6A) were identified by means of allele-specific PCR. Results of the study included a significant increase of mRNA for MIP-1α, eotaxin 2, along with decreased amounts of mRNA for CCR-3 (a specific receptor for eotaxins), in polyps developing from hyperplastic endometrium. MIP-1α synthesis fades away with increasing age. An increased level of MIP-1β was shown in prolonged and recurrent disturbances of menstrual cycle, whereas elevation of MIP-1α and CXCR-1 was registered in cases of multiple pregnancies. In threatening abortions, an increase of MIP-1β gene expression was revealed. Hence, the local chemokine system reacts to inflammatory and hemorrhagic complications with increased mRNA expression of certain chemokine genes. Determination of the chemokine mRNA levels, as well as their receptors in patients with endometrial hyperplasia may reflect a general background of this disorder. (Med. Immunol., 2011, vol. 13, N 2-3, pp 189-196)
format	article
author	N. V. Kipich N. V. Kulagina A. B. Chukhlovin K. A. Syssoev Areg A. Totolian
author_facet	N. V. Kipich N. V. Kulagina A. B. Chukhlovin K. A. Syssoev Areg A. Totolian
author_sort	N. V. Kipich
title	FEATURES OF LOCAL mRNA SYNTHESIS FOR SOME CC- AND CXC-CHEMOKINES AND THEIR RECEPTORS IN ENDOMETRIAL HYPERPLASIA
title_short	FEATURES OF LOCAL mRNA SYNTHESIS FOR SOME CC- AND CXC-CHEMOKINES AND THEIR RECEPTORS IN ENDOMETRIAL HYPERPLASIA
title_full	FEATURES OF LOCAL mRNA SYNTHESIS FOR SOME CC- AND CXC-CHEMOKINES AND THEIR RECEPTORS IN ENDOMETRIAL HYPERPLASIA
title_fullStr	FEATURES OF LOCAL mRNA SYNTHESIS FOR SOME CC- AND CXC-CHEMOKINES AND THEIR RECEPTORS IN ENDOMETRIAL HYPERPLASIA
title_full_unstemmed	FEATURES OF LOCAL mRNA SYNTHESIS FOR SOME CC- AND CXC-CHEMOKINES AND THEIR RECEPTORS IN ENDOMETRIAL HYPERPLASIA
title_sort	features of local mrna synthesis for some cc- and cxc-chemokines and their receptors in endometrial hyperplasia
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/523cff2a577249c197b272c7f14e9035
work_keys_str_mv	AT nvkipich featuresoflocalmrnasynthesisforsomeccandcxcchemokinesandtheirreceptorsinendometrialhyperplasia AT nvkulagina featuresoflocalmrnasynthesisforsomeccandcxcchemokinesandtheirreceptorsinendometrialhyperplasia AT abchukhlovin featuresoflocalmrnasynthesisforsomeccandcxcchemokinesandtheirreceptorsinendometrialhyperplasia AT kasyssoev featuresoflocalmrnasynthesisforsomeccandcxcchemokinesandtheirreceptorsinendometrialhyperplasia AT aregatotolian featuresoflocalmrnasynthesisforsomeccandcxcchemokinesandtheirreceptorsinendometrialhyperplasia
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FEATURES OF LOCAL mRNA SYNTHESIS FOR SOME CC- AND CXC-CHEMOKINES AND THEIR RECEPTORS IN ENDOMETRIAL HYPERPLASIA

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