Overexpression of Ras homologous C (RhoC) induces malignant transformation of hepatocytes in vitro and in nude mouse xenografts.

Ras homologous C (RhoC) is expressed in various cancers, including hepatocellular carcinoma (HCC). In this study, we first analyzed RhoC expression in 46 HCC tissue specimens and found that RhoC expression was significantly increased in HCC tissues compared to the adjacent normal liver tissues. Next...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shuli Xie, Mingguang Zhu, Guoyue Lv, Yajun Geng, Guofu Chen, Jian Ma, Guangyi Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5242798e24b34c619429c65d9ebfa73c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5242798e24b34c619429c65d9ebfa73c
record_format	dspace
spelling	oai:doaj.org-article:5242798e24b34c619429c65d9ebfa73c2021-11-18T07:59:33ZOverexpression of Ras homologous C (RhoC) induces malignant transformation of hepatocytes in vitro and in nude mouse xenografts.1932-620310.1371/journal.pone.0054493https://doaj.org/article/5242798e24b34c619429c65d9ebfa73c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23382905/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Ras homologous C (RhoC) is expressed in various cancers, including hepatocellular carcinoma (HCC). In this study, we first analyzed RhoC expression in 46 HCC tissue specimens and found that RhoC expression was significantly increased in HCC tissues compared to the adjacent normal liver tissues. Next, we investigated the role of RhoC in malignant transformation of normal hepatocytes. The HL7702 cell line was stably transfected with a RhoC expression vector and then subjected to cell proliferation, differentiation, colony formation, migration and invasion assays, as well as nude mouse xenograft assays. Gene expressions in these cells were determined using RT-PCR and Western blot. Overexpression of RhoC significantly promoted proliferation and anchorage-independent growth of HL7702 cells, but suppressed cell differentiation, as compared with the parental cells and the empty vector-transfected control cells. Moreover, RhoC overexpression induced migration and invasion of HL7702 cells in vitro. Molecularly, RhoC increased the expression of cell cycle-related genes, matrix metalloprotease 2 (MMP2), MMP9 and vascular endothelial growth factor (VEGF). In addition, RhoC-transfected cells formed tumors in nude mice, whereas vector-transfected HL7702 cells did not form any tumors in nude mice. This study demonstrated the role of RhoC overexpression in malignant transformation of normal human hepatocytes, suggesting that RhoC may function as an oncogene in hepatocytes.Shuli XieMingguang ZhuGuoyue LvYajun GengGuofu ChenJian MaGuangyi WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54493 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuli Xie Mingguang Zhu Guoyue Lv Yajun Geng Guofu Chen Jian Ma Guangyi Wang Overexpression of Ras homologous C (RhoC) induces malignant transformation of hepatocytes in vitro and in nude mouse xenografts.
description	Ras homologous C (RhoC) is expressed in various cancers, including hepatocellular carcinoma (HCC). In this study, we first analyzed RhoC expression in 46 HCC tissue specimens and found that RhoC expression was significantly increased in HCC tissues compared to the adjacent normal liver tissues. Next, we investigated the role of RhoC in malignant transformation of normal hepatocytes. The HL7702 cell line was stably transfected with a RhoC expression vector and then subjected to cell proliferation, differentiation, colony formation, migration and invasion assays, as well as nude mouse xenograft assays. Gene expressions in these cells were determined using RT-PCR and Western blot. Overexpression of RhoC significantly promoted proliferation and anchorage-independent growth of HL7702 cells, but suppressed cell differentiation, as compared with the parental cells and the empty vector-transfected control cells. Moreover, RhoC overexpression induced migration and invasion of HL7702 cells in vitro. Molecularly, RhoC increased the expression of cell cycle-related genes, matrix metalloprotease 2 (MMP2), MMP9 and vascular endothelial growth factor (VEGF). In addition, RhoC-transfected cells formed tumors in nude mice, whereas vector-transfected HL7702 cells did not form any tumors in nude mice. This study demonstrated the role of RhoC overexpression in malignant transformation of normal human hepatocytes, suggesting that RhoC may function as an oncogene in hepatocytes.
format	article
author	Shuli Xie Mingguang Zhu Guoyue Lv Yajun Geng Guofu Chen Jian Ma Guangyi Wang
author_facet	Shuli Xie Mingguang Zhu Guoyue Lv Yajun Geng Guofu Chen Jian Ma Guangyi Wang
author_sort	Shuli Xie
title	Overexpression of Ras homologous C (RhoC) induces malignant transformation of hepatocytes in vitro and in nude mouse xenografts.
title_short	Overexpression of Ras homologous C (RhoC) induces malignant transformation of hepatocytes in vitro and in nude mouse xenografts.
title_full	Overexpression of Ras homologous C (RhoC) induces malignant transformation of hepatocytes in vitro and in nude mouse xenografts.
title_fullStr	Overexpression of Ras homologous C (RhoC) induces malignant transformation of hepatocytes in vitro and in nude mouse xenografts.
title_full_unstemmed	Overexpression of Ras homologous C (RhoC) induces malignant transformation of hepatocytes in vitro and in nude mouse xenografts.
title_sort	overexpression of ras homologous c (rhoc) induces malignant transformation of hepatocytes in vitro and in nude mouse xenografts.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5242798e24b34c619429c65d9ebfa73c
work_keys_str_mv	AT shulixie overexpressionofrashomologouscrhocinducesmalignanttransformationofhepatocytesinvitroandinnudemousexenografts AT mingguangzhu overexpressionofrashomologouscrhocinducesmalignanttransformationofhepatocytesinvitroandinnudemousexenografts AT guoyuelv overexpressionofrashomologouscrhocinducesmalignanttransformationofhepatocytesinvitroandinnudemousexenografts AT yajungeng overexpressionofrashomologouscrhocinducesmalignanttransformationofhepatocytesinvitroandinnudemousexenografts AT guofuchen overexpressionofrashomologouscrhocinducesmalignanttransformationofhepatocytesinvitroandinnudemousexenografts AT jianma overexpressionofrashomologouscrhocinducesmalignanttransformationofhepatocytesinvitroandinnudemousexenografts AT guangyiwang overexpressionofrashomologouscrhocinducesmalignanttransformationofhepatocytesinvitroandinnudemousexenografts
_version_	1718422664066891776

Overexpression of Ras homologous C (RhoC) induces malignant transformation of hepatocytes in vitro and in nude mouse xenografts.

Ejemplares similares