Mapping B-cell epitopes for the peroxidoxin of Leishmania (Viannia) braziliensis and its potential for the clinical diagnosis of tegumentary and visceral leishmaniasis.

The search toward the establishment of novel serological tests for the diagnosis of leishmaniasis and proper differential diagnosis may represent one alternative to the invasive parasitological methods currently used to identify infected individuals. In the present work, we investigated the potentia...

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Autores principales:	Daniel Menezes-Souza, Tiago Antônio de Oliveira Mendes, Ronaldo Alves Pinto Nagem, Thaís Teodoro de Oliveira Santos, Ana Luíza Teixeira Silva, Marcelo Matos Santoro, Silvio Fernando Guimarães de Carvalho, Eduardo Antônio Ferraz Coelho, Daniella Castanheira Bartholomeu, Ricardo Toshio Fujiwara
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/524b819fa52a499abcd1b3c570b26dfb
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spelling	oai:doaj.org-article:524b819fa52a499abcd1b3c570b26dfb2021-11-18T08:16:02ZMapping B-cell epitopes for the peroxidoxin of Leishmania (Viannia) braziliensis and its potential for the clinical diagnosis of tegumentary and visceral leishmaniasis.1932-620310.1371/journal.pone.0099216https://doaj.org/article/524b819fa52a499abcd1b3c570b26dfb2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24921246/?tool=EBIhttps://doaj.org/toc/1932-6203The search toward the establishment of novel serological tests for the diagnosis of leishmaniasis and proper differential diagnosis may represent one alternative to the invasive parasitological methods currently used to identify infected individuals. In the present work, we investigated the potential use of recombinant peroxidoxin (rPeroxidoxin) of Leishmania (Viannia) braziliensis as a potential antigen for the immunodiagnosis of human tegumentary (TL) and visceral leishmaniasis (VL) and canine visceral leishmaniasis (CVL). Linear B-cell epitope mapping was performed to identify polymorphic epitopes when comparing orthologous sequences present in Trypanosoma cruzi, the agent for Chagas disease (CD), and the Homo sapiens and Canis familiaris hosts. The serological assay (ELISA) demonstrated that TL, VL and CVL individuals showed high levels of antibodies against rPeroxidoxin, allowing identification of infected ones with considerable sensitivity and great ability to discriminate (specificity) between non-infected and CD individuals (98.46% and 100%; 98.18% and 95.71%; 95.79% and 100%, respectively). An rPeroxidoxin ELISA also showed a greater ability to discriminate between vaccinated and infected animals, which is an important requirement for the public campaign control of CVL. A depletion ELISA assay using soluble peptides of this B-cell epitope confirmed the recognition of these sites only by Leishmania-infected individuals. Moreover, this work identifies two antigenic polymorphic linear B-cell epitopes of L. braziliensis. Specific recognition of TL and VL patients was confirmed by significantly decreased IgG reactivity against rPeroxidoxin after depletion of peptide-1- and peptide-2-specific antibodies (peptide 1: reduced by 32%, 42% and 5% for CL, ML and VL, respectively; peptide-2: reduced by 24%, 22% and 13% for CL, ML and VL, respectively) and only peptide-2 for CVL (reduced 9%). Overall, rPeroxidoxin may be a potential antigen for the immunodiagnosis of TL, VL or CVL, as it has a higher agreement with parasitological assays and is better than other reference tests that use soluble Leishmania antigens for diagnosing CVL in Brazil (EIE-LVC, Bio-manguinhos, FIOCRUZ).Daniel Menezes-SouzaTiago Antônio de Oliveira MendesRonaldo Alves Pinto NagemThaís Teodoro de Oliveira SantosAna Luíza Teixeira SilvaMarcelo Matos SantoroSilvio Fernando Guimarães de CarvalhoEduardo Antônio Ferraz CoelhoDaniella Castanheira BartholomeuRicardo Toshio FujiwaraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99216 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Menezes-Souza Tiago Antônio de Oliveira Mendes Ronaldo Alves Pinto Nagem Thaís Teodoro de Oliveira Santos Ana Luíza Teixeira Silva Marcelo Matos Santoro Silvio Fernando Guimarães de Carvalho Eduardo Antônio Ferraz Coelho Daniella Castanheira Bartholomeu Ricardo Toshio Fujiwara Mapping B-cell epitopes for the peroxidoxin of Leishmania (Viannia) braziliensis and its potential for the clinical diagnosis of tegumentary and visceral leishmaniasis.
description	The search toward the establishment of novel serological tests for the diagnosis of leishmaniasis and proper differential diagnosis may represent one alternative to the invasive parasitological methods currently used to identify infected individuals. In the present work, we investigated the potential use of recombinant peroxidoxin (rPeroxidoxin) of Leishmania (Viannia) braziliensis as a potential antigen for the immunodiagnosis of human tegumentary (TL) and visceral leishmaniasis (VL) and canine visceral leishmaniasis (CVL). Linear B-cell epitope mapping was performed to identify polymorphic epitopes when comparing orthologous sequences present in Trypanosoma cruzi, the agent for Chagas disease (CD), and the Homo sapiens and Canis familiaris hosts. The serological assay (ELISA) demonstrated that TL, VL and CVL individuals showed high levels of antibodies against rPeroxidoxin, allowing identification of infected ones with considerable sensitivity and great ability to discriminate (specificity) between non-infected and CD individuals (98.46% and 100%; 98.18% and 95.71%; 95.79% and 100%, respectively). An rPeroxidoxin ELISA also showed a greater ability to discriminate between vaccinated and infected animals, which is an important requirement for the public campaign control of CVL. A depletion ELISA assay using soluble peptides of this B-cell epitope confirmed the recognition of these sites only by Leishmania-infected individuals. Moreover, this work identifies two antigenic polymorphic linear B-cell epitopes of L. braziliensis. Specific recognition of TL and VL patients was confirmed by significantly decreased IgG reactivity against rPeroxidoxin after depletion of peptide-1- and peptide-2-specific antibodies (peptide 1: reduced by 32%, 42% and 5% for CL, ML and VL, respectively; peptide-2: reduced by 24%, 22% and 13% for CL, ML and VL, respectively) and only peptide-2 for CVL (reduced 9%). Overall, rPeroxidoxin may be a potential antigen for the immunodiagnosis of TL, VL or CVL, as it has a higher agreement with parasitological assays and is better than other reference tests that use soluble Leishmania antigens for diagnosing CVL in Brazil (EIE-LVC, Bio-manguinhos, FIOCRUZ).
format	article
author	Daniel Menezes-Souza Tiago Antônio de Oliveira Mendes Ronaldo Alves Pinto Nagem Thaís Teodoro de Oliveira Santos Ana Luíza Teixeira Silva Marcelo Matos Santoro Silvio Fernando Guimarães de Carvalho Eduardo Antônio Ferraz Coelho Daniella Castanheira Bartholomeu Ricardo Toshio Fujiwara
author_facet	Daniel Menezes-Souza Tiago Antônio de Oliveira Mendes Ronaldo Alves Pinto Nagem Thaís Teodoro de Oliveira Santos Ana Luíza Teixeira Silva Marcelo Matos Santoro Silvio Fernando Guimarães de Carvalho Eduardo Antônio Ferraz Coelho Daniella Castanheira Bartholomeu Ricardo Toshio Fujiwara
author_sort	Daniel Menezes-Souza
title	Mapping B-cell epitopes for the peroxidoxin of Leishmania (Viannia) braziliensis and its potential for the clinical diagnosis of tegumentary and visceral leishmaniasis.
title_short	Mapping B-cell epitopes for the peroxidoxin of Leishmania (Viannia) braziliensis and its potential for the clinical diagnosis of tegumentary and visceral leishmaniasis.
title_full	Mapping B-cell epitopes for the peroxidoxin of Leishmania (Viannia) braziliensis and its potential for the clinical diagnosis of tegumentary and visceral leishmaniasis.
title_fullStr	Mapping B-cell epitopes for the peroxidoxin of Leishmania (Viannia) braziliensis and its potential for the clinical diagnosis of tegumentary and visceral leishmaniasis.
title_full_unstemmed	Mapping B-cell epitopes for the peroxidoxin of Leishmania (Viannia) braziliensis and its potential for the clinical diagnosis of tegumentary and visceral leishmaniasis.
title_sort	mapping b-cell epitopes for the peroxidoxin of leishmania (viannia) braziliensis and its potential for the clinical diagnosis of tegumentary and visceral leishmaniasis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/524b819fa52a499abcd1b3c570b26dfb
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Mapping B-cell epitopes for the peroxidoxin of Leishmania (Viannia) braziliensis and its potential for the clinical diagnosis of tegumentary and visceral leishmaniasis.

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