Fully integrated rapid microfluidic device translated from conventional 96-well ELISA kit

Abstract In this work, a fully integrated active microfluidic device transforming a conventional 96-well kit into point-of-care testing (POCT) device was implemented to improve the performance of traditional enzyme-linked immunosorbent assay (ELISA). ELISA test by the conventional method often requi...

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Autores principales:	M. Jalal Uddin, Nabil H. Bhuiyan, Joon S. Shim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/524bb530c88745feb4581dcd0b8d9d4f
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spelling	oai:doaj.org-article:524bb530c88745feb4581dcd0b8d9d4f2021-12-02T10:49:30ZFully integrated rapid microfluidic device translated from conventional 96-well ELISA kit10.1038/s41598-021-81433-y2045-2322https://doaj.org/article/524bb530c88745feb4581dcd0b8d9d4f2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81433-yhttps://doaj.org/toc/2045-2322Abstract In this work, a fully integrated active microfluidic device transforming a conventional 96-well kit into point-of-care testing (POCT) device was implemented to improve the performance of traditional enzyme-linked immunosorbent assay (ELISA). ELISA test by the conventional method often requires the collection of 96 samples for its operation as well as longer incubation time from hours to overnight, whereas our proposed device conducts ELISA immediately individualizing a 96-well for individual patients. To do that, a programmable and disposable on-chip pump and valve were integrated on the device for precise control and actuation of microfluidic reagents, which regulated a reaction time and reagent volume to support the optimized protocols of ELISA. Due to the on-chip pump and valve, ELISA could be executed with reduced consumption of reagents and shortening the assay time, which are crucial for conventional ELISA using 96-well microplate. To demonstrate highly sensitive detection and easy-to-use operation, this unconventional device was successfully applied for the quantification of cardiac troponin I (cTnI) of 4.88 pg/mL using a minimum sample volume of 30 µL with a shorter assay time of 15 min for each ELISA step. The limit of detection (LOD) thus obtained was significantly improved than the conventional 96-well platform.M. Jalal UddinNabil H. BhuiyanJoon S. ShimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Jalal Uddin Nabil H. Bhuiyan Joon S. Shim Fully integrated rapid microfluidic device translated from conventional 96-well ELISA kit
description	Abstract In this work, a fully integrated active microfluidic device transforming a conventional 96-well kit into point-of-care testing (POCT) device was implemented to improve the performance of traditional enzyme-linked immunosorbent assay (ELISA). ELISA test by the conventional method often requires the collection of 96 samples for its operation as well as longer incubation time from hours to overnight, whereas our proposed device conducts ELISA immediately individualizing a 96-well for individual patients. To do that, a programmable and disposable on-chip pump and valve were integrated on the device for precise control and actuation of microfluidic reagents, which regulated a reaction time and reagent volume to support the optimized protocols of ELISA. Due to the on-chip pump and valve, ELISA could be executed with reduced consumption of reagents and shortening the assay time, which are crucial for conventional ELISA using 96-well microplate. To demonstrate highly sensitive detection and easy-to-use operation, this unconventional device was successfully applied for the quantification of cardiac troponin I (cTnI) of 4.88 pg/mL using a minimum sample volume of 30 µL with a shorter assay time of 15 min for each ELISA step. The limit of detection (LOD) thus obtained was significantly improved than the conventional 96-well platform.
format	article
author	M. Jalal Uddin Nabil H. Bhuiyan Joon S. Shim
author_facet	M. Jalal Uddin Nabil H. Bhuiyan Joon S. Shim
author_sort	M. Jalal Uddin
title	Fully integrated rapid microfluidic device translated from conventional 96-well ELISA kit
title_short	Fully integrated rapid microfluidic device translated from conventional 96-well ELISA kit
title_full	Fully integrated rapid microfluidic device translated from conventional 96-well ELISA kit
title_fullStr	Fully integrated rapid microfluidic device translated from conventional 96-well ELISA kit
title_full_unstemmed	Fully integrated rapid microfluidic device translated from conventional 96-well ELISA kit
title_sort	fully integrated rapid microfluidic device translated from conventional 96-well elisa kit
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/524bb530c88745feb4581dcd0b8d9d4f
work_keys_str_mv	AT mjalaluddin fullyintegratedrapidmicrofluidicdevicetranslatedfromconventional96wellelisakit AT nabilhbhuiyan fullyintegratedrapidmicrofluidicdevicetranslatedfromconventional96wellelisakit AT joonsshim fullyintegratedrapidmicrofluidicdevicetranslatedfromconventional96wellelisakit
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Fully integrated rapid microfluidic device translated from conventional 96-well ELISA kit

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