Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt

Many Mycoplasma species are involved in caprine and ovine pneumonia all over the world causing serious economic losses. These species have been reported to cause pneumonia, mastitis, arthritis, conjunctivitis and genital infection. The objective of the present study was to detect Mycoplasma species...

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Autores principales:	Mona, M. Osman, Manal Abu Elmakarem Mohamed, Heba, N. Deif, Kamelia, M. Osman
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	biofilm-formation goat sheep untyped-mycoplasma-species virulence-genes Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2021.88356.1095 https://doaj.org/article/524cf8d967bd4b88b01db300242acf1d
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spelling	oai:doaj.org-article:524cf8d967bd4b88b01db300242acf1d2021-12-02T17:12:50ZVirulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypthttps://dx.doi.org/10.21608/javs.2021.88356.10951687-40722090-3308https://doaj.org/article/524cf8d967bd4b88b01db300242acf1d2021-10-01T00:00:00Zhttps://javs.journals.ekb.eg/article_192838.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Many Mycoplasma species are involved in caprine and ovine pneumonia all over the world causing serious economic losses. These species have been reported to cause pneumonia, mastitis, arthritis, conjunctivitis and genital infection. The objective of the present study was to detect Mycoplasma species in the respiratory system of sheep and goats. So, a total of 400 samples (250 from sheep and 150 from goat) were examined bacteriologically, biochemically, by PCR, sequencing, in addition to the antibiotic susceptibility profiles screening. Only 13 isolates could be identified as Mycoplasma species. One of these sequences was submitted to the Genbank taking the accession number MK910041; Mycoplasma species 'ovine/caprine serogroup 11'. Only 7 isolates were weak biofilm producers and the other 6 isolates were non biofilm producers; 8 isolates were positive for the catalase test and 5 were negative. H2S production was recorded in 10 isolates; haemolysis was detected in only 6 isolates. In addition, the minimum inhibitory concentrations (MICs) for seven antimicrobial agents, including danofloxacin, tulathromycin, tylosin, streptomycin, lincomycin, florfenicol and oxytetracycline, were determined. All the isolates (100%) were sensitive to tulathromycin and tylosin, streptomycin and oxytetracycline; 38.5% were sensitive to danofloxacin, 69.2% were sensitive to florfenicol, while 69.2% of isolates were resistant to lincomycin. As all the isolates were recovered from apparently healthy and clinically diseased animals and identified as untyped Mycoplasma species, more investigations will be done to identify these isolates and discover their roles in infection.Mona, M. OsmanManal Abu Elmakarem MohamedHeba, N. Deif Kamelia, M. OsmanEgyptian Society for Animal Managementarticlebiofilm-formationgoatsheepuntyped-mycoplasma-speciesvirulence-genesZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 4, Pp 39-45 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biofilm-formation goat sheep untyped-mycoplasma-species virulence-genes Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	biofilm-formation goat sheep untyped-mycoplasma-species virulence-genes Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Mona, M. Osman Manal Abu Elmakarem Mohamed Heba, N. Deif Kamelia, M. Osman Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt
description	Many Mycoplasma species are involved in caprine and ovine pneumonia all over the world causing serious economic losses. These species have been reported to cause pneumonia, mastitis, arthritis, conjunctivitis and genital infection. The objective of the present study was to detect Mycoplasma species in the respiratory system of sheep and goats. So, a total of 400 samples (250 from sheep and 150 from goat) were examined bacteriologically, biochemically, by PCR, sequencing, in addition to the antibiotic susceptibility profiles screening. Only 13 isolates could be identified as Mycoplasma species. One of these sequences was submitted to the Genbank taking the accession number MK910041; Mycoplasma species 'ovine/caprine serogroup 11'. Only 7 isolates were weak biofilm producers and the other 6 isolates were non biofilm producers; 8 isolates were positive for the catalase test and 5 were negative. H2S production was recorded in 10 isolates; haemolysis was detected in only 6 isolates. In addition, the minimum inhibitory concentrations (MICs) for seven antimicrobial agents, including danofloxacin, tulathromycin, tylosin, streptomycin, lincomycin, florfenicol and oxytetracycline, were determined. All the isolates (100%) were sensitive to tulathromycin and tylosin, streptomycin and oxytetracycline; 38.5% were sensitive to danofloxacin, 69.2% were sensitive to florfenicol, while 69.2% of isolates were resistant to lincomycin. As all the isolates were recovered from apparently healthy and clinically diseased animals and identified as untyped Mycoplasma species, more investigations will be done to identify these isolates and discover their roles in infection.
format	article
author	Mona, M. Osman Manal Abu Elmakarem Mohamed Heba, N. Deif Kamelia, M. Osman
author_facet	Mona, M. Osman Manal Abu Elmakarem Mohamed Heba, N. Deif Kamelia, M. Osman
author_sort	Mona, M. Osman
title	Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt
title_short	Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt
title_full	Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt
title_fullStr	Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt
title_full_unstemmed	Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt
title_sort	virulence traits and antimicrobial sensitivity testing of untyped mycoplasma species recovered from sheep and goat in egypt
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://dx.doi.org/10.21608/javs.2021.88356.1095 https://doaj.org/article/524cf8d967bd4b88b01db300242acf1d
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Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt

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