Local mechanical stimuli regulate bone formation and resorption in mice at the tissue level.

Bone is able to react to changing mechanical demands by adapting its internal microstructure through bone forming and resorbing cells. This process is called bone modeling and remodeling. It is evident that changes in mechanical demands at the organ level must be interpreted at the tissue level wher...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Friederike A Schulte, Davide Ruffoni, Floor M Lambers, David Christen, Duncan J Webster, Gisela Kuhn, Ralph Müller
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/524e02452c844fda97f21d915456c285
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:524e02452c844fda97f21d915456c285
record_format	dspace
spelling	oai:doaj.org-article:524e02452c844fda97f21d915456c2852021-11-18T07:47:59ZLocal mechanical stimuli regulate bone formation and resorption in mice at the tissue level.1932-620310.1371/journal.pone.0062172https://doaj.org/article/524e02452c844fda97f21d915456c2852013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23637993/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Bone is able to react to changing mechanical demands by adapting its internal microstructure through bone forming and resorbing cells. This process is called bone modeling and remodeling. It is evident that changes in mechanical demands at the organ level must be interpreted at the tissue level where bone (re)modeling takes place. Although assumed for a long time, the relationship between the locations of bone formation and resorption and the local mechanical environment is still under debate. The lack of suitable imaging modalities for measuring bone formation and resorption in vivo has made it difficult to assess the mechanoregulation of bone three-dimensionally by experiment. Using in vivo micro-computed tomography and high resolution finite element analysis in living mice, we show that bone formation most likely occurs at sites of high local mechanical strain (p<0.0001) and resorption at sites of low local mechanical strain (p<0.0001). Furthermore, the probability of bone resorption decreases exponentially with increasing mechanical stimulus (R(2) = 0.99) whereas the probability of bone formation follows an exponential growth function to a maximum value (R(2) = 0.99). Moreover, resorption is more strictly controlled than formation in loaded animals, and ovariectomy increases the amount of non-targeted resorption. Our experimental assessment of mechanoregulation at the tissue level does not show any evidence of a lazy zone and suggests that around 80% of all (re)modeling can be linked to the mechanical micro-environment. These findings disclose how mechanical stimuli at the tissue level contribute to the regulation of bone adaptation at the organ level.Friederike A SchulteDavide RuffoniFloor M LambersDavid ChristenDuncan J WebsterGisela KuhnRalph MüllerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62172 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Friederike A Schulte Davide Ruffoni Floor M Lambers David Christen Duncan J Webster Gisela Kuhn Ralph Müller Local mechanical stimuli regulate bone formation and resorption in mice at the tissue level.
description	Bone is able to react to changing mechanical demands by adapting its internal microstructure through bone forming and resorbing cells. This process is called bone modeling and remodeling. It is evident that changes in mechanical demands at the organ level must be interpreted at the tissue level where bone (re)modeling takes place. Although assumed for a long time, the relationship between the locations of bone formation and resorption and the local mechanical environment is still under debate. The lack of suitable imaging modalities for measuring bone formation and resorption in vivo has made it difficult to assess the mechanoregulation of bone three-dimensionally by experiment. Using in vivo micro-computed tomography and high resolution finite element analysis in living mice, we show that bone formation most likely occurs at sites of high local mechanical strain (p<0.0001) and resorption at sites of low local mechanical strain (p<0.0001). Furthermore, the probability of bone resorption decreases exponentially with increasing mechanical stimulus (R(2) = 0.99) whereas the probability of bone formation follows an exponential growth function to a maximum value (R(2) = 0.99). Moreover, resorption is more strictly controlled than formation in loaded animals, and ovariectomy increases the amount of non-targeted resorption. Our experimental assessment of mechanoregulation at the tissue level does not show any evidence of a lazy zone and suggests that around 80% of all (re)modeling can be linked to the mechanical micro-environment. These findings disclose how mechanical stimuli at the tissue level contribute to the regulation of bone adaptation at the organ level.
format	article
author	Friederike A Schulte Davide Ruffoni Floor M Lambers David Christen Duncan J Webster Gisela Kuhn Ralph Müller
author_facet	Friederike A Schulte Davide Ruffoni Floor M Lambers David Christen Duncan J Webster Gisela Kuhn Ralph Müller
author_sort	Friederike A Schulte
title	Local mechanical stimuli regulate bone formation and resorption in mice at the tissue level.
title_short	Local mechanical stimuli regulate bone formation and resorption in mice at the tissue level.
title_full	Local mechanical stimuli regulate bone formation and resorption in mice at the tissue level.
title_fullStr	Local mechanical stimuli regulate bone formation and resorption in mice at the tissue level.
title_full_unstemmed	Local mechanical stimuli regulate bone formation and resorption in mice at the tissue level.
title_sort	local mechanical stimuli regulate bone formation and resorption in mice at the tissue level.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/524e02452c844fda97f21d915456c285
work_keys_str_mv	AT friederikeaschulte localmechanicalstimuliregulateboneformationandresorptioninmiceatthetissuelevel AT davideruffoni localmechanicalstimuliregulateboneformationandresorptioninmiceatthetissuelevel AT floormlambers localmechanicalstimuliregulateboneformationandresorptioninmiceatthetissuelevel AT davidchristen localmechanicalstimuliregulateboneformationandresorptioninmiceatthetissuelevel AT duncanjwebster localmechanicalstimuliregulateboneformationandresorptioninmiceatthetissuelevel AT giselakuhn localmechanicalstimuliregulateboneformationandresorptioninmiceatthetissuelevel AT ralphmuller localmechanicalstimuliregulateboneformationandresorptioninmiceatthetissuelevel
_version_	1718422910015635456

Local mechanical stimuli regulate bone formation and resorption in mice at the tissue level.

Ejemplares similares