Effect of Human Burn Wound Exudate on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Virulence

ABSTRACT Burn wound sepsis is currently the main cause of morbidity and mortality after burn trauma. Infections by notorious pathogens such as Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter baumannii impair patient recovery and can even lead to fatality. In this study, we investiga...

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Autores principales:	Manuel R. Gonzalez, Betty Fleuchot, Leonardo Lauciello, Paris Jafari, Lee Ann Applegate, Wassim Raffoul, Yok-Ai Que, Karl Perron
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	burn wound exudate Pseudomonas aeruginosa virulence factors growth Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5250008a009d48f79021a377acae4cf3
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spelling	oai:doaj.org-article:5250008a009d48f79021a377acae4cf32021-11-15T15:21:22ZEffect of Human Burn Wound Exudate on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Virulence10.1128/mSphere.00111-152379-5042https://doaj.org/article/5250008a009d48f79021a377acae4cf32016-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00111-15https://doaj.org/toc/2379-5042ABSTRACT Burn wound sepsis is currently the main cause of morbidity and mortality after burn trauma. Infections by notorious pathogens such as Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter baumannii impair patient recovery and can even lead to fatality. In this study, we investigated the effect of burn wound exudates (BWEs) on the virulence of those pathogens. BWEs were collected within 7 days after burn trauma from 5 burn patients. We first monitored their effect on pathogen growth. In contrast to A. baumannii and S. aureus, P. aeruginosa was the only pathogen able to grow within these human fluids. Expression of typical virulence factors such as pyocyanin and pyoverdine was even enhanced compared the levels seen with standard laboratory medium. A detailed chemical composition analysis of BWE was performed, which enabled us to determine the major components of BWE and underline the metabolic modifications induced by burn trauma. These data are essential for the development of an artificial medium mimicking the burn wound environment and the establishment of an in vitro system to analyze the initial steps of burn wound infections. IMPORTANCE Microbial infection of severe burn wounds is currently a major medical challenge. Of the infections by bacteria able to colonize such injuries, those by Pseudomonas aeruginosa are among the most severe, causing major delays in burn patient recovery or leading to fatal issues. In this study, we investigated the growth properties of several burn wound pathogens in biological fluids secreted from human burn wounds. We found that P. aeruginosa strains were able to proliferate but not those of the other pathogens tested. In addition, burn wound exudates (BWEs) stimulate the expression of virulence factors in P. aeruginosa. The chemical composition analysis of BWEs enabled us to determine the major components of these fluids. These data are essential for the development of an artificial medium mimicking the burn wound environment and for in vitro analysis of the initial step in the development of burn wound infections.Manuel R. GonzalezBetty FleuchotLeonardo LaucielloParis JafariLee Ann ApplegateWassim RaffoulYok-Ai QueKarl PerronAmerican Society for Microbiologyarticleburn wound exudatePseudomonas aeruginosavirulence factorsgrowthMicrobiologyQR1-502ENmSphere, Vol 1, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	burn wound exudate Pseudomonas aeruginosa virulence factors growth Microbiology QR1-502
spellingShingle	burn wound exudate Pseudomonas aeruginosa virulence factors growth Microbiology QR1-502 Manuel R. Gonzalez Betty Fleuchot Leonardo Lauciello Paris Jafari Lee Ann Applegate Wassim Raffoul Yok-Ai Que Karl Perron Effect of Human Burn Wound Exudate on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Virulence
description	ABSTRACT Burn wound sepsis is currently the main cause of morbidity and mortality after burn trauma. Infections by notorious pathogens such as Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter baumannii impair patient recovery and can even lead to fatality. In this study, we investigated the effect of burn wound exudates (BWEs) on the virulence of those pathogens. BWEs were collected within 7 days after burn trauma from 5 burn patients. We first monitored their effect on pathogen growth. In contrast to A. baumannii and S. aureus, P. aeruginosa was the only pathogen able to grow within these human fluids. Expression of typical virulence factors such as pyocyanin and pyoverdine was even enhanced compared the levels seen with standard laboratory medium. A detailed chemical composition analysis of BWE was performed, which enabled us to determine the major components of BWE and underline the metabolic modifications induced by burn trauma. These data are essential for the development of an artificial medium mimicking the burn wound environment and the establishment of an in vitro system to analyze the initial steps of burn wound infections. IMPORTANCE Microbial infection of severe burn wounds is currently a major medical challenge. Of the infections by bacteria able to colonize such injuries, those by Pseudomonas aeruginosa are among the most severe, causing major delays in burn patient recovery or leading to fatal issues. In this study, we investigated the growth properties of several burn wound pathogens in biological fluids secreted from human burn wounds. We found that P. aeruginosa strains were able to proliferate but not those of the other pathogens tested. In addition, burn wound exudates (BWEs) stimulate the expression of virulence factors in P. aeruginosa. The chemical composition analysis of BWEs enabled us to determine the major components of these fluids. These data are essential for the development of an artificial medium mimicking the burn wound environment and for in vitro analysis of the initial step in the development of burn wound infections.
format	article
author	Manuel R. Gonzalez Betty Fleuchot Leonardo Lauciello Paris Jafari Lee Ann Applegate Wassim Raffoul Yok-Ai Que Karl Perron
author_facet	Manuel R. Gonzalez Betty Fleuchot Leonardo Lauciello Paris Jafari Lee Ann Applegate Wassim Raffoul Yok-Ai Que Karl Perron
author_sort	Manuel R. Gonzalez
title	Effect of Human Burn Wound Exudate on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Virulence
title_short	Effect of Human Burn Wound Exudate on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Virulence
title_full	Effect of Human Burn Wound Exudate on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Virulence
title_fullStr	Effect of Human Burn Wound Exudate on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Virulence
title_full_unstemmed	Effect of Human Burn Wound Exudate on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Virulence
title_sort	effect of human burn wound exudate on <named-content content-type="genus-species">pseudomonas aeruginosa</named-content> virulence
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/5250008a009d48f79021a377acae4cf3
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Effect of Human Burn Wound Exudate on <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Virulence

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