Utility of neutrophil CD64 in distinguishing bacterial infection from inflammation in severe alcoholic hepatitis fulfilling SIRS criteria
Abstract To assess utility of neutrophilCD64 (nCD64) expression in differentiating bacterial infection from inflammation in patients with severe alcoholic hepatitis (SAH) fulfilling systemic inflammatory response syndrome criteria. Patients with SAH and infection (n = 58), SAH without infection (n =...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/5259080f1cff4b22b1f20b573c7ce12d |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:5259080f1cff4b22b1f20b573c7ce12d |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:5259080f1cff4b22b1f20b573c7ce12d2021-12-02T19:16:18ZUtility of neutrophil CD64 in distinguishing bacterial infection from inflammation in severe alcoholic hepatitis fulfilling SIRS criteria10.1038/s41598-021-99276-y2045-2322https://doaj.org/article/5259080f1cff4b22b1f20b573c7ce12d2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99276-yhttps://doaj.org/toc/2045-2322Abstract To assess utility of neutrophilCD64 (nCD64) expression in differentiating bacterial infection from inflammation in patients with severe alcoholic hepatitis (SAH) fulfilling systemic inflammatory response syndrome criteria. Patients with SAH and infection (n = 58), SAH without infection (n = 70), and healthy controls (n = 20) were included. Neutrophil CD64 expression by flowcytometry, serum Procalcitonin (ELISA) and C-reactive protein (Nephelometry) and neutrophil–lymphocyte ratio (NLR) were studied. Percentage of neutrophils with CD64 expression (nCD64%) was significantly higher in patients with SAH and infection than in those without infection and controls [76.2% (56.9–86.5) vs. 16% (12.6–23.1) vs. 7.05% (1.4–9.5), p < 0.05], as was their mean fluorescence intensity [MFI; 1431 (229–1828) vs. 853 (20–968) vs. 99.5 (54.7–140.7), p < 0.05]. Using a cut-off of 27%, the sensitivity and specificity of nCD64% to diagnose bacterial infection was 94% and 81%, respectively, with area under curve (AUC) of 0.95. At a cut-off value of 0.261 ng/ml, the sensitivity and specificity of serum procalcitonin was 83% and 72%, respectively, with AUC of 0.86. Serum CRP, total leukocyte count, NLR had AUCs of 0.78, 0.63 and 0.64, respectively. Quantitative measurement of nCD64 can better distinguish systemic bacterial infection and inflammation in SAH as compared to traditional biomarkers.Gaurav PandeyHarshit SinghSaurabh ChaturvediManjunath HattiAlok KumarRavi MishraPrabhakar MishraV. P. KrishnaArun BhadauriaSamir MohindraDurga Prasanna MisraVivek Anand SaraswatVikas AgarwalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Gaurav Pandey Harshit Singh Saurabh Chaturvedi Manjunath Hatti Alok Kumar Ravi Mishra Prabhakar Mishra V. P. Krishna Arun Bhadauria Samir Mohindra Durga Prasanna Misra Vivek Anand Saraswat Vikas Agarwal Utility of neutrophil CD64 in distinguishing bacterial infection from inflammation in severe alcoholic hepatitis fulfilling SIRS criteria |
| description |
Abstract To assess utility of neutrophilCD64 (nCD64) expression in differentiating bacterial infection from inflammation in patients with severe alcoholic hepatitis (SAH) fulfilling systemic inflammatory response syndrome criteria. Patients with SAH and infection (n = 58), SAH without infection (n = 70), and healthy controls (n = 20) were included. Neutrophil CD64 expression by flowcytometry, serum Procalcitonin (ELISA) and C-reactive protein (Nephelometry) and neutrophil–lymphocyte ratio (NLR) were studied. Percentage of neutrophils with CD64 expression (nCD64%) was significantly higher in patients with SAH and infection than in those without infection and controls [76.2% (56.9–86.5) vs. 16% (12.6–23.1) vs. 7.05% (1.4–9.5), p < 0.05], as was their mean fluorescence intensity [MFI; 1431 (229–1828) vs. 853 (20–968) vs. 99.5 (54.7–140.7), p < 0.05]. Using a cut-off of 27%, the sensitivity and specificity of nCD64% to diagnose bacterial infection was 94% and 81%, respectively, with area under curve (AUC) of 0.95. At a cut-off value of 0.261 ng/ml, the sensitivity and specificity of serum procalcitonin was 83% and 72%, respectively, with AUC of 0.86. Serum CRP, total leukocyte count, NLR had AUCs of 0.78, 0.63 and 0.64, respectively. Quantitative measurement of nCD64 can better distinguish systemic bacterial infection and inflammation in SAH as compared to traditional biomarkers. |
| format |
article |
| author |
Gaurav Pandey Harshit Singh Saurabh Chaturvedi Manjunath Hatti Alok Kumar Ravi Mishra Prabhakar Mishra V. P. Krishna Arun Bhadauria Samir Mohindra Durga Prasanna Misra Vivek Anand Saraswat Vikas Agarwal |
| author_facet |
Gaurav Pandey Harshit Singh Saurabh Chaturvedi Manjunath Hatti Alok Kumar Ravi Mishra Prabhakar Mishra V. P. Krishna Arun Bhadauria Samir Mohindra Durga Prasanna Misra Vivek Anand Saraswat Vikas Agarwal |
| author_sort |
Gaurav Pandey |
| title |
Utility of neutrophil CD64 in distinguishing bacterial infection from inflammation in severe alcoholic hepatitis fulfilling SIRS criteria |
| title_short |
Utility of neutrophil CD64 in distinguishing bacterial infection from inflammation in severe alcoholic hepatitis fulfilling SIRS criteria |
| title_full |
Utility of neutrophil CD64 in distinguishing bacterial infection from inflammation in severe alcoholic hepatitis fulfilling SIRS criteria |
| title_fullStr |
Utility of neutrophil CD64 in distinguishing bacterial infection from inflammation in severe alcoholic hepatitis fulfilling SIRS criteria |
| title_full_unstemmed |
Utility of neutrophil CD64 in distinguishing bacterial infection from inflammation in severe alcoholic hepatitis fulfilling SIRS criteria |
| title_sort |
utility of neutrophil cd64 in distinguishing bacterial infection from inflammation in severe alcoholic hepatitis fulfilling sirs criteria |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/5259080f1cff4b22b1f20b573c7ce12d |
| work_keys_str_mv |
AT gauravpandey utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria AT harshitsingh utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria AT saurabhchaturvedi utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria AT manjunathhatti utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria AT alokkumar utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria AT ravimishra utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria AT prabhakarmishra utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria AT vpkrishna utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria AT arunbhadauria utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria AT samirmohindra utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria AT durgaprasannamisra utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria AT vivekanandsaraswat utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria AT vikasagarwal utilityofneutrophilcd64indistinguishingbacterialinfectionfrominflammationinseverealcoholichepatitisfulfillingsirscriteria |
| _version_ |
1718376969949675520 |