Biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury

Abstract Traumatic brain injury (TBI) is the main cause of disability and mortality in individuals under the age of 45 years. Elucidation of the molecular and structural alterations in brain tissue due to TBI is crucial to understand secondary and long-term effects after traumatic brain injury, and...

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Autores principales:	Sebnem Garip Ustaoglu, Mohamed H. M. Ali, Fazle Rakib, Erwin L. A. Blezer, Caroline L. Van Heijningen, Rick M. Dijkhuizen, Feride Severcan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/525aee62480946b2bda8b48fec28bc20
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spelling	oai:doaj.org-article:525aee62480946b2bda8b48fec28bc202021-12-02T17:23:03ZBiomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury10.1038/s41598-021-92015-32045-2322https://doaj.org/article/525aee62480946b2bda8b48fec28bc202021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92015-3https://doaj.org/toc/2045-2322Abstract Traumatic brain injury (TBI) is the main cause of disability and mortality in individuals under the age of 45 years. Elucidation of the molecular and structural alterations in brain tissue due to TBI is crucial to understand secondary and long-term effects after traumatic brain injury, and to develop and apply the correct therapies. In the current study, the molecular effects of TBI were investigated in rat brain at 24 h and 1 month after the injury to determine acute and chronic effects, respectively by Fourier transform infrared imaging. This study reports the time-dependent contextual and structural effects of TBI on hippocampal brain tissue. A mild form of TBI was induced in 11-week old male Sprague Dawley rats by weight drop. Band area and intensity ratios, band frequency and bandwidth values of specific spectral bands showed that TBI causes significant structural and contextual global changes including decrease in carbonyl content, unsaturated lipid content, lipid acyl chain length, membrane lipid order, total protein content, lipid/protein ratio, besides increase in membrane fluidity with an altered protein secondary structure and metabolic activity in hippocampus 24 h after injury. However, improvement and/or recovery effects in these parameters were observed at one month after TBI.Sebnem Garip UstaogluMohamed H. M. AliFazle RakibErwin L. A. BlezerCaroline L. Van HeijningenRick M. DijkhuizenFeride SevercanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sebnem Garip Ustaoglu Mohamed H. M. Ali Fazle Rakib Erwin L. A. Blezer Caroline L. Van Heijningen Rick M. Dijkhuizen Feride Severcan Biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury
description	Abstract Traumatic brain injury (TBI) is the main cause of disability and mortality in individuals under the age of 45 years. Elucidation of the molecular and structural alterations in brain tissue due to TBI is crucial to understand secondary and long-term effects after traumatic brain injury, and to develop and apply the correct therapies. In the current study, the molecular effects of TBI were investigated in rat brain at 24 h and 1 month after the injury to determine acute and chronic effects, respectively by Fourier transform infrared imaging. This study reports the time-dependent contextual and structural effects of TBI on hippocampal brain tissue. A mild form of TBI was induced in 11-week old male Sprague Dawley rats by weight drop. Band area and intensity ratios, band frequency and bandwidth values of specific spectral bands showed that TBI causes significant structural and contextual global changes including decrease in carbonyl content, unsaturated lipid content, lipid acyl chain length, membrane lipid order, total protein content, lipid/protein ratio, besides increase in membrane fluidity with an altered protein secondary structure and metabolic activity in hippocampus 24 h after injury. However, improvement and/or recovery effects in these parameters were observed at one month after TBI.
format	article
author	Sebnem Garip Ustaoglu Mohamed H. M. Ali Fazle Rakib Erwin L. A. Blezer Caroline L. Van Heijningen Rick M. Dijkhuizen Feride Severcan
author_facet	Sebnem Garip Ustaoglu Mohamed H. M. Ali Fazle Rakib Erwin L. A. Blezer Caroline L. Van Heijningen Rick M. Dijkhuizen Feride Severcan
author_sort	Sebnem Garip Ustaoglu
title	Biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury
title_short	Biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury
title_full	Biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury
title_fullStr	Biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury
title_full_unstemmed	Biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury
title_sort	biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/525aee62480946b2bda8b48fec28bc20
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Biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury

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