Deficient spindle assembly checkpoint in multiple myeloma.

Multiple myeloma (MM) is a hematological disease characterized by an abnormal accumulation of plasma cells in the bone marrow. These cells have frequent cytogenetic abnormalities including translocations of the immunoglobulin heavy chain gene and chromosomal gains and losses. In fact, a singular cha...

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Autores principales:	Elena Díaz-Rodríguez, Stela Álvarez-Fernández, Xi Chen, Bruno Paiva, Ricardo López-Pérez, Juan Luis García-Hernández, Jesús F San Miguel, Atanasio Pandiella
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/525aefe7784b42099ba9f17f23b9e505
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spelling	oai:doaj.org-article:525aefe7784b42099ba9f17f23b9e5052021-11-18T07:33:39ZDeficient spindle assembly checkpoint in multiple myeloma.1932-620310.1371/journal.pone.0027583https://doaj.org/article/525aefe7784b42099ba9f17f23b9e5052011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22132115/?tool=EBIhttps://doaj.org/toc/1932-6203Multiple myeloma (MM) is a hematological disease characterized by an abnormal accumulation of plasma cells in the bone marrow. These cells have frequent cytogenetic abnormalities including translocations of the immunoglobulin heavy chain gene and chromosomal gains and losses. In fact, a singular characteristic differentiating MM from other hematological malignancies is the presence of a high degree of aneuploidies. As chromosomal abnormalities can be generated by alterations in the spindle assembly checkpoint (SAC), the functionality of such checkpoint was tested in MM. When SAC components were analyzed in MM cell lines, the RNA levels of most of them were conserved. Nevertheless, the protein content of some key constituents was very low in several cell lines, as was the case of MAD2 or CDC20 in RPMI-8226 or RPMI-LR5 cells. The recovery of their cellular content did not substantially affect cell growth, but improved their ability to segregate chromosomes. Finally, SAC functionality was tested by challenging cells with agents disrupting microtubule dynamics. Most of the cell lines analyzed exhibited functional defects in this checkpoint. Based on the data obtained, alterations both in SAC components and their functionality have been detected in MM, pointing to this pathway as a potential target in MM treatment.Elena Díaz-RodríguezStela Álvarez-FernándezXi ChenBruno PaivaRicardo López-PérezJuan Luis García-HernándezJesús F San MiguelAtanasio PandiellaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27583 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elena Díaz-Rodríguez Stela Álvarez-Fernández Xi Chen Bruno Paiva Ricardo López-Pérez Juan Luis García-Hernández Jesús F San Miguel Atanasio Pandiella Deficient spindle assembly checkpoint in multiple myeloma.
description	Multiple myeloma (MM) is a hematological disease characterized by an abnormal accumulation of plasma cells in the bone marrow. These cells have frequent cytogenetic abnormalities including translocations of the immunoglobulin heavy chain gene and chromosomal gains and losses. In fact, a singular characteristic differentiating MM from other hematological malignancies is the presence of a high degree of aneuploidies. As chromosomal abnormalities can be generated by alterations in the spindle assembly checkpoint (SAC), the functionality of such checkpoint was tested in MM. When SAC components were analyzed in MM cell lines, the RNA levels of most of them were conserved. Nevertheless, the protein content of some key constituents was very low in several cell lines, as was the case of MAD2 or CDC20 in RPMI-8226 or RPMI-LR5 cells. The recovery of their cellular content did not substantially affect cell growth, but improved their ability to segregate chromosomes. Finally, SAC functionality was tested by challenging cells with agents disrupting microtubule dynamics. Most of the cell lines analyzed exhibited functional defects in this checkpoint. Based on the data obtained, alterations both in SAC components and their functionality have been detected in MM, pointing to this pathway as a potential target in MM treatment.
format	article
author	Elena Díaz-Rodríguez Stela Álvarez-Fernández Xi Chen Bruno Paiva Ricardo López-Pérez Juan Luis García-Hernández Jesús F San Miguel Atanasio Pandiella
author_facet	Elena Díaz-Rodríguez Stela Álvarez-Fernández Xi Chen Bruno Paiva Ricardo López-Pérez Juan Luis García-Hernández Jesús F San Miguel Atanasio Pandiella
author_sort	Elena Díaz-Rodríguez
title	Deficient spindle assembly checkpoint in multiple myeloma.
title_short	Deficient spindle assembly checkpoint in multiple myeloma.
title_full	Deficient spindle assembly checkpoint in multiple myeloma.
title_fullStr	Deficient spindle assembly checkpoint in multiple myeloma.
title_full_unstemmed	Deficient spindle assembly checkpoint in multiple myeloma.
title_sort	deficient spindle assembly checkpoint in multiple myeloma.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/525aefe7784b42099ba9f17f23b9e505
work_keys_str_mv	AT elenadiazrodriguez deficientspindleassemblycheckpointinmultiplemyeloma AT stelaalvarezfernandez deficientspindleassemblycheckpointinmultiplemyeloma AT xichen deficientspindleassemblycheckpointinmultiplemyeloma AT brunopaiva deficientspindleassemblycheckpointinmultiplemyeloma AT ricardolopezperez deficientspindleassemblycheckpointinmultiplemyeloma AT juanluisgarciahernandez deficientspindleassemblycheckpointinmultiplemyeloma AT jesusfsanmiguel deficientspindleassemblycheckpointinmultiplemyeloma AT atanasiopandiella deficientspindleassemblycheckpointinmultiplemyeloma
_version_	1718423283990265856

Deficient spindle assembly checkpoint in multiple myeloma.

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