Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages

Although the neonatal and fetal pathogen Group B Streptococcus (GBS) asymptomatically colonizes the vaginal tract of ∼30% of pregnant women, only a fraction of their offspring develops invasive disease. We and others have postulated that these dimorphic clinical phenotypes are driven by strain varia...

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Autores principales:	Cole R. McCutcheon, Macy E. Pell, Jennifer A. Gaddy, David M. Aronoff, Margaret G. Petroff, Shannon D. Manning
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Streptococcus agalactiae membrane vesicles virulence pathogenesis proteomics group B Streptococcus Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/526121ad7f7e4963ba344e04654c8181
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spelling	oai:doaj.org-article:526121ad7f7e4963ba344e04654c81812021-11-22T06:28:31ZProduction and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages1664-302X10.3389/fmicb.2021.770499https://doaj.org/article/526121ad7f7e4963ba344e04654c81812021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.770499/fullhttps://doaj.org/toc/1664-302XAlthough the neonatal and fetal pathogen Group B Streptococcus (GBS) asymptomatically colonizes the vaginal tract of ∼30% of pregnant women, only a fraction of their offspring develops invasive disease. We and others have postulated that these dimorphic clinical phenotypes are driven by strain variability; however, the bacterial factors that promote these divergent clinical phenotypes remain unclear. It was previously shown that GBS produces membrane vesicles (MVs) that contain active virulence factors capable of inducing adverse pregnancy outcomes. Because the relationship between strain variation and vesicle composition or production is unknown, we sought to quantify MV production and examine the protein composition, using label-free proteomics on MVs produced by diverse clinical GBS strains representing three phylogenetically distinct lineages. We found that MV production varied across strains, with certain strains displaying nearly twofold increases in production relative to others. Hierarchical clustering and principal component analysis of the proteomes revealed that MV composition is lineage-dependent but independent of clinical phenotype. Multiple proteins that contribute to virulence or immunomodulation, including hyaluronidase, C5a peptidase, and sialidases, were differentially abundant in MVs, and were partially responsible for this divergence. Together, these data indicate that production and composition of GBS MVs vary in a strain-dependent manner, suggesting that MVs have lineage-specific functions relating to virulence. Such differences may contribute to variation in clinical phenotypes observed among individuals infected with GBS strains representing distinct lineages.Cole R. McCutcheonMacy E. PellJennifer A. GaddyJennifer A. GaddyJennifer A. GaddyDavid M. AronoffDavid M. AronoffDavid M. AronoffMargaret G. PetroffMargaret G. PetroffShannon D. ManningFrontiers Media S.A.articleStreptococcus agalactiaemembrane vesiclesvirulencepathogenesisproteomicsgroup B StreptococcusMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Streptococcus agalactiae membrane vesicles virulence pathogenesis proteomics group B Streptococcus Microbiology QR1-502
spellingShingle	Streptococcus agalactiae membrane vesicles virulence pathogenesis proteomics group B Streptococcus Microbiology QR1-502 Cole R. McCutcheon Macy E. Pell Jennifer A. Gaddy Jennifer A. Gaddy Jennifer A. Gaddy David M. Aronoff David M. Aronoff David M. Aronoff Margaret G. Petroff Margaret G. Petroff Shannon D. Manning Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages
description	Although the neonatal and fetal pathogen Group B Streptococcus (GBS) asymptomatically colonizes the vaginal tract of ∼30% of pregnant women, only a fraction of their offspring develops invasive disease. We and others have postulated that these dimorphic clinical phenotypes are driven by strain variability; however, the bacterial factors that promote these divergent clinical phenotypes remain unclear. It was previously shown that GBS produces membrane vesicles (MVs) that contain active virulence factors capable of inducing adverse pregnancy outcomes. Because the relationship between strain variation and vesicle composition or production is unknown, we sought to quantify MV production and examine the protein composition, using label-free proteomics on MVs produced by diverse clinical GBS strains representing three phylogenetically distinct lineages. We found that MV production varied across strains, with certain strains displaying nearly twofold increases in production relative to others. Hierarchical clustering and principal component analysis of the proteomes revealed that MV composition is lineage-dependent but independent of clinical phenotype. Multiple proteins that contribute to virulence or immunomodulation, including hyaluronidase, C5a peptidase, and sialidases, were differentially abundant in MVs, and were partially responsible for this divergence. Together, these data indicate that production and composition of GBS MVs vary in a strain-dependent manner, suggesting that MVs have lineage-specific functions relating to virulence. Such differences may contribute to variation in clinical phenotypes observed among individuals infected with GBS strains representing distinct lineages.
format	article
author	Cole R. McCutcheon Macy E. Pell Jennifer A. Gaddy Jennifer A. Gaddy Jennifer A. Gaddy David M. Aronoff David M. Aronoff David M. Aronoff Margaret G. Petroff Margaret G. Petroff Shannon D. Manning
author_facet	Cole R. McCutcheon Macy E. Pell Jennifer A. Gaddy Jennifer A. Gaddy Jennifer A. Gaddy David M. Aronoff David M. Aronoff David M. Aronoff Margaret G. Petroff Margaret G. Petroff Shannon D. Manning
author_sort	Cole R. McCutcheon
title	Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages
title_short	Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages
title_full	Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages
title_fullStr	Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages
title_full_unstemmed	Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages
title_sort	production and composition of group b streptococcal membrane vesicles vary across diverse lineages
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/526121ad7f7e4963ba344e04654c8181
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Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages

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