Combined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice

Abstract In this study, the toxic effects of aflatoxin B2 (AFB2) on Swiss albino mice and the protective effects of resveratrol were investigated. Physiological (body weight, liver and kidney weight), biochemical (aspartate aminotransferase-AST, alanine transaminase-ALT, blood urea nitrogen-BUN, cre...

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Autores principales:	Alperen Gündüz, Emine Yalçın, Kültiğin Çavuşoğlu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5269cfa6fc564d74858027806ac8af60
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spelling	oai:doaj.org-article:5269cfa6fc564d74858027806ac8af602021-12-02T17:19:16ZCombined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice10.1038/s41598-021-95879-72045-2322https://doaj.org/article/5269cfa6fc564d74858027806ac8af602021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95879-7https://doaj.org/toc/2045-2322Abstract In this study, the toxic effects of aflatoxin B2 (AFB2) on Swiss albino mice and the protective effects of resveratrol were investigated. Physiological (body weight, liver and kidney weight), biochemical (aspartate aminotransferase-AST, alanine transaminase-ALT, blood urea nitrogen-BUN, creatinine, malondialdehyde-MDA and glutathione-GSH) and cytogenetic parameters (micronucleus-MN in buccal epithelium, erythrocyte and leukocyte cells and chromosomal aberrations-CAs) were used to determine the toxic effects. Additionally, scavenging effects of resveratrol against superoxide, hydrogen peroxide (H2O2) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were also investigated. In experimental period, mice were divided into six groups and the groups were treated with tap water, 10 mg/kg b.w resveratrol, 20 mg/kg b.w resveratrol, 20 µg/kg b.w. AFB2, 10 mg/kg b.w resveratrol + 20 µg/kg b.w AFB2, 20 mg/kg b.w resveratrol + 20 µg/kg b.w AFB2, respectively. As a result, the scavenging effects of resveratrol increased with increasing dose and the superoxide, H2O2 and DPPH radical scavenging activity of resveratrol were 74.9%, 79.1% and 49.2%, respectively. AFB2 administration caused a significant decrease in physiological parameters, and these decreases regressed in AFB2 + resveratrol treated groups. Serum ALT and AST activities, BUN and creatinine levels were higher in the AFB2 treated group compared to the control group and serious abnormalities were found in MDA and GSH levels in the kidney and liver. In the group treated with AFB2 + 20 mg/kg resveratrol, ALT, AST, BUN and creatinine levels decreased significantly and GSH levels increased compared to only-AFB2 treated group. AFB2 triggered MN formation in buccal epithelium, erythrocyte and leukocyte cells and CAs in bone marrow cells. The application of 20 mg/kg resveratrol together with AFB2 was decreased the MN and CAs frequency. Resveratrol exhibited a recovery effect in the range of 40.9–80.5% against AFB2 toxicity in all tested parameters. In this study, it was determined that AFB2 caused serious changes in selected physiological, biochemical and cytogenetic parameters while resveratrol displayed a protective role against these toxic effects.Alperen GündüzEmine YalçınKültiğin ÇavuşoğluNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alperen Gündüz Emine Yalçın Kültiğin Çavuşoğlu Combined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice
description	Abstract In this study, the toxic effects of aflatoxin B2 (AFB2) on Swiss albino mice and the protective effects of resveratrol were investigated. Physiological (body weight, liver and kidney weight), biochemical (aspartate aminotransferase-AST, alanine transaminase-ALT, blood urea nitrogen-BUN, creatinine, malondialdehyde-MDA and glutathione-GSH) and cytogenetic parameters (micronucleus-MN in buccal epithelium, erythrocyte and leukocyte cells and chromosomal aberrations-CAs) were used to determine the toxic effects. Additionally, scavenging effects of resveratrol against superoxide, hydrogen peroxide (H2O2) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were also investigated. In experimental period, mice were divided into six groups and the groups were treated with tap water, 10 mg/kg b.w resveratrol, 20 mg/kg b.w resveratrol, 20 µg/kg b.w. AFB2, 10 mg/kg b.w resveratrol + 20 µg/kg b.w AFB2, 20 mg/kg b.w resveratrol + 20 µg/kg b.w AFB2, respectively. As a result, the scavenging effects of resveratrol increased with increasing dose and the superoxide, H2O2 and DPPH radical scavenging activity of resveratrol were 74.9%, 79.1% and 49.2%, respectively. AFB2 administration caused a significant decrease in physiological parameters, and these decreases regressed in AFB2 + resveratrol treated groups. Serum ALT and AST activities, BUN and creatinine levels were higher in the AFB2 treated group compared to the control group and serious abnormalities were found in MDA and GSH levels in the kidney and liver. In the group treated with AFB2 + 20 mg/kg resveratrol, ALT, AST, BUN and creatinine levels decreased significantly and GSH levels increased compared to only-AFB2 treated group. AFB2 triggered MN formation in buccal epithelium, erythrocyte and leukocyte cells and CAs in bone marrow cells. The application of 20 mg/kg resveratrol together with AFB2 was decreased the MN and CAs frequency. Resveratrol exhibited a recovery effect in the range of 40.9–80.5% against AFB2 toxicity in all tested parameters. In this study, it was determined that AFB2 caused serious changes in selected physiological, biochemical and cytogenetic parameters while resveratrol displayed a protective role against these toxic effects.
format	article
author	Alperen Gündüz Emine Yalçın Kültiğin Çavuşoğlu
author_facet	Alperen Gündüz Emine Yalçın Kültiğin Çavuşoğlu
author_sort	Alperen Gündüz
title	Combined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice
title_short	Combined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice
title_full	Combined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice
title_fullStr	Combined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice
title_full_unstemmed	Combined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice
title_sort	combined toxic effects of aflatoxin b2 and the protective role of resveratrol in swiss albino mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5269cfa6fc564d74858027806ac8af60
work_keys_str_mv	AT alperengunduz combinedtoxiceffectsofaflatoxinb2andtheprotectiveroleofresveratrolinswissalbinomice AT emineyalcın combinedtoxiceffectsofaflatoxinb2andtheprotectiveroleofresveratrolinswissalbinomice AT kultigincavusoglu combinedtoxiceffectsofaflatoxinb2andtheprotectiveroleofresveratrolinswissalbinomice
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Combined toxic effects of aflatoxin B2 and the protective role of resveratrol in Swiss albino mice

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