Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat

Abstract Plants deploy several ammonium transporter (AMT) and nitrate transporter (NRT) genes to acquire NH4 + and NO3 − from the soil into the roots and then transport them to other plant organs. Coding sequences of wheat genes obtained from ENSEMBL were aligned to known AMT and NRT sequences of Ar...

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Autores principales:	Prabin Bajgain, Blake Russell, Mohsen Mohammadi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/526c94d742854ceeba5f6a9d6f9bd2fd
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spelling	oai:doaj.org-article:526c94d742854ceeba5f6a9d6f9bd2fd2021-12-02T11:41:03ZPhylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat10.1038/s41598-018-25430-82045-2322https://doaj.org/article/526c94d742854ceeba5f6a9d6f9bd2fd2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25430-8https://doaj.org/toc/2045-2322Abstract Plants deploy several ammonium transporter (AMT) and nitrate transporter (NRT) genes to acquire NH4 + and NO3 − from the soil into the roots and then transport them to other plant organs. Coding sequences of wheat genes obtained from ENSEMBL were aligned to known AMT and NRT sequences of Arabidopsis, barley, maize, rice, and wheat to retrieve homologous genes. Bayesian phylogenetic relationships among these genes showed distinct classification of sequences with significant homology to NRT1, NRT2, and NRT3 (NAR2). Inter-species gene duplication analysis showed that eight AMT and 77 NRT genes were orthologous to the AMT and NRT genes of aforementioned plant species. Expression patterns of these genes were studied via whole transcriptome sequencing of 21-day old seedlings of five spring wheat lines. Eight AMT and 52 NRT genes were differentially expressed between root and shoot; and 131 genes did not express neither in root nor in shoot of 21-day old seedlings. Homeologous genes in the A, B, and D genomes, characterized by high sequence homology, revealed that their counterparts exhibited different expression patterns. This complement and evolutionary relationship of wheat AMT and NRT genes is expected to help in development of wheat germplasm with increased efficiency in nitrogen uptake and usage.Prabin BajgainBlake RussellMohsen MohammadiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Prabin Bajgain Blake Russell Mohsen Mohammadi Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
description	Abstract Plants deploy several ammonium transporter (AMT) and nitrate transporter (NRT) genes to acquire NH4 + and NO3 − from the soil into the roots and then transport them to other plant organs. Coding sequences of wheat genes obtained from ENSEMBL were aligned to known AMT and NRT sequences of Arabidopsis, barley, maize, rice, and wheat to retrieve homologous genes. Bayesian phylogenetic relationships among these genes showed distinct classification of sequences with significant homology to NRT1, NRT2, and NRT3 (NAR2). Inter-species gene duplication analysis showed that eight AMT and 77 NRT genes were orthologous to the AMT and NRT genes of aforementioned plant species. Expression patterns of these genes were studied via whole transcriptome sequencing of 21-day old seedlings of five spring wheat lines. Eight AMT and 52 NRT genes were differentially expressed between root and shoot; and 131 genes did not express neither in root nor in shoot of 21-day old seedlings. Homeologous genes in the A, B, and D genomes, characterized by high sequence homology, revealed that their counterparts exhibited different expression patterns. This complement and evolutionary relationship of wheat AMT and NRT genes is expected to help in development of wheat germplasm with increased efficiency in nitrogen uptake and usage.
format	article
author	Prabin Bajgain Blake Russell Mohsen Mohammadi
author_facet	Prabin Bajgain Blake Russell Mohsen Mohammadi
author_sort	Prabin Bajgain
title	Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
title_short	Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
title_full	Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
title_fullStr	Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
title_full_unstemmed	Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
title_sort	phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/526c94d742854ceeba5f6a9d6f9bd2fd
work_keys_str_mv	AT prabinbajgain phylogeneticanalysesandinseedlingexpressionofammoniumandnitratetransportersinwheat AT blakerussell phylogeneticanalysesandinseedlingexpressionofammoniumandnitratetransportersinwheat AT mohsenmohammadi phylogeneticanalysesandinseedlingexpressionofammoniumandnitratetransportersinwheat
_version_	1718395460228481024

Phylogenetic analyses and in-seedling expression of ammonium and nitrate transporters in wheat

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