Optimization of extraction of circulating RNAs from plasma--enabling small RNA sequencing.
There are several protocols and kits for the extraction of circulating RNAs from plasma with a following quantification of specific genes via RT-qPCR. Due to the marginal amount of cell-free RNA in plasma samples, the total RNA yield is insufficient to perform Next-Generation Sequencing (NGS), the s...
Guardado en:
Autores principales: | Melanie Spornraft, Benedikt Kirchner, Bettina Haase, Vladimir Benes, Michael W Pfaffl, Irmgard Riedmaier |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2014
|
Materias: | |
Acceso en línea: | https://doaj.org/article/5272204a5ba445689dc74329bdfed01a |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
Circulating tRNA-derived small RNAs (tsRNAs) signature for the diagnosis and prognosis of breast cancer
por: Jingyi Wang, et al.
Publicado: (2021) -
Circulating Exosomal miRNAs as Novel Biomarkers Perform Superior Diagnostic Efficiency Compared With Plasma miRNAs for Large-Artery Atherosclerosis Stroke
por: Mengying Niu, et al.
Publicado: (2021) -
Circulating microRNAs in Fabry Disease
por: Ke Xiao, et al.
Publicado: (2019) -
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
por: Roman S. Iwasaki, et al.
Publicado: (2020) -
Hydro-Seq enables contamination-free high-throughput single-cell RNA-sequencing for circulating tumor cells
por: Yu-Heng Cheng, et al.
Publicado: (2019)